川中黑山羊和雷州山羊大小卵泡转录组学分析

doi:10.16431/j.cnki.1671-7236.2020.05.020

Abstract

Abstract: This study was aimed to excavate the genes affecting follicular development with the mRNA transcriptome difference between large and small follicles in two different goats,in order to provide a reference for further elucidating the mechanism of goat follicular development.Synchronization of estrus was carried out on the Chuanzhong Black and Leizhou goats with cloprostenol sodium.The single follicle (large follicle>6 mm,small follicle<3 mm) was isolated from ovaries which were collected from estrus goats mentioned above with the stereomicroscope.The total RNA was extracted from follicle tissues for RNA-seq,the profiles of mRNA expression were calculated with bioinformatics and differentially expressed genes (DEGs) were performed in GO function and KEGG pathway enrichment.The results showed that 4 451 DEmRNAs were obtained from different follicles in Chuanzhong Black goats,and 2 335 DEmRNAs were obtained from Leizhou goats,two goat breeds 1 771 genes.The common (INHBA,INHA,CYP19A1,KITLG,LHCGR and STAR) and unique (IGFBP6,BMP6 and BMPR2) genes related to follicular development were screened out.In addition,the new genes (GADD45B,TC2N and MSMO1) potentially affecting the reproductive performance in two goat breeds were also identified.GO function analysis showed that the DEGs in two goat breeds were mainly related to the transmembrane transport activity.KEGG pathway analysis revealed significant changes in steroidogenesis,cytokine-cytokine receptor interaction and cAMP signaling in both goat breeds.It was also found that the difference of cytochrome P450 metabolism in steroidogenesis when follicle developed could be a potential factor in the high litter size of Chuanzhong Black goats.The results provided a reference for further exploring the gene functions of follicle development and the research in reproductive performance differences of different goat breeds.

Key words: goats; follicle; transcriptome; gene

CLC Number:

S814.8

ZHAO Zhifeng, ZOU Xian, LIU Guangbin, YANG Xinyue, LIAN Zhiquan, GUO Yongqing, LIU Dewu. Comparative Transcriptome Analysis Between Large and Small Follicles in Chuanzhong Black and Leizhou Goats[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1459-1471.

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Comparative Transcriptome Analysis Between Large and Small Follicles in Chuanzhong Black and Leizhou Goats

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