抗菌肽添加量对杂交鲟生化指标的影响

doi:10.16431/j.cnki.1671-7236.2020.05.019

Abstract

Abstract: In order to study the effect of the amount of antimicrobial peptide on the immunity-related indicators and anti-injury ability of the serum and tissue of hybrid sturgeon.200 hybrid sturgeons(Russian sturgeon Acipenser gueldenstaedti♀×Amur sturgeon Acipenser schrencki Brandt♂) with robust physique and uniform size were selected and randomly divided into 4 groups.The fishes in control group were fed with basal diet,the fishes in experiment groups were fed with composite additive of antimicrobial peptides with level of 400 mg/kg (group Ⅰ)、800 mg/kg (group Ⅱ) and 1 200 mg/kg (group Ⅲ),respectively.The experiment lasted for 30 d at water temperature of (16.0±1.0) ℃.It was found that the contents of albumin (ALB) and total protein(TP) in serum and tissues were increased at first and then decreased with the increase of dietary antimicrobial peptides concentration,and the contents of ALB and TP in groupⅡ significantly higher than the control group (P<0.05).The activities of lactic dehydrogenase (LDH) and glutamic oxalacetic transaminase (GOT) were shown to be decreased at first and then increased with the increase of antimicrobial peptides concentration in serum.In serum and tissues the activity of GOT in groupⅠwas significantly lower than that in other groups (P<0.05),and the activity of GOT and LDH in group Ⅲ were significantly higher than those in other groups (P<0.05).The total cholesterol (TCHO) contents were gradually decreased with the increase of the concentration of antimicrobial peptides in tissues,and the TCHO contents in group Ⅲ were significantly lower in tissues than those in other groups (P<0.05).The activity of lysozyme (LZM) was gradually increased with the increase of the concentration of antimicrobial peptides,and the activity of LZM was significantly higher in groupⅡ and groupⅢ in heart,spleen and small intestine than that in other groups (P<0.05).The addition of antimicrobial peptides leaded to effectively improve the nonspecific immunity and the damage resistant ability of hybrid sturgeon,and antibacterial peptides had the best effect when the dosage was 400-800 mg/kg.

Key words: antimicrobial peptide; biochemical index; hybrid sturgeon; immunity ability

CLC Number:

S966.9

LUO Zhicheng, LIU Zhe, QUAN Jinqiang, KANG Yujun, QIN Yong. Effects of Additive Amount of Antimicrobial Peptides on Biochemical Indices of Hybrid Sturgeon[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(5): 1452-1458.

References

[1] 孙大江,曲秋芝,张颖,等.中国的鲟鱼养殖[J].水产学杂志,2011,24(4):67-70. SUN D J,QU Q Z,ZHANG Y,et al.Sturgeon aquaculture in China[J].Chinese Journal of Fisheries,2011,24(4):67-70.(in Chinese)
[2] 陈细华,李创举,杨长庚,等.中国鲟鱼产业技术研发现状与展望[J].淡水渔业,2017,47(6):108-112. CHEN X H,LI C J,YANG C G.Status and prospects of techniques in the sturgeon aquaculture industry in China[J].Freshwater Fisheries,2017,47(6):108-112.(in Chinese)
[3] KAROLY L.Physiologic and pathophysiologic significance of antimicrobial (host defensive) small peptides[J].Orvosi Hetilap,2009,149(51):2419-2424.
[4] REDDY K V R,YEDERY R D,ARANHA C.Antimicrobial peptides:Premises and promises[J].International Journal of Antimicrobial Agents,2004,24(6):536-547.
[5] HIRAKURA Y,KOBAYASHI S,MATSUZAKI K.Specific interactions of the antimicrobial peptide cyclic beta-sheet tachyplesin Ⅰ with lipopolysaccharides[J].Biochimica et Biophysica Acta,2002,1562(1):32-36.
[6] 李冠楠,夏雪娟,隆耀航,等.抗菌肽的研究进展及其应用[J].动物营养学报,2014,26(1):17-25. LI G N,XIA X J,LONG Y H,et al.Research progress and application of antimicrobial peptides[J].Chinese Journal of Animal Nutrition,2014,26(1):17-25.(in Chinese)
[7] LI Y M,XIANG Q,ZHANG Q H.Overview on the recent study of antimicrobial peptides:Origins,functions,relative mechanisms and application[J].Peptides (New York),2012,37(2):207-215.
[8] 刘莉如,杨开伦,滑静,等.抗菌肽对海兰褐仔公鸡小肠黏膜形态结构及免疫活性细胞数量的影响[J].动物营养学报,2013,25(1):190-197. LIU L R,YANG K L,HUA J,et al.Antimicrobial peptides:Effects on small intestinal mucosal morphology and immune active cell number in hy-line brown young roosters[J].Chinese Journal of Animal Nutrition,2013,25(1):190-197.(in Chinese)
[9] 丁修良,赵建飞,王帅,等.抗菌肽Sublancin对肉鸡生长性能、养分利用及盲肠菌群的影响[J].动物营养学报,2018,30(7):2690-2699. DING X L,ZHAO J F,WANG S,et al.Effect of antimicrobial peptide sublancin on growth performance,nutrient utilization and cecal flora of broilers[J].Chinese Journal of Animal Nutrition,2018,30(7):2690-2699.(in Chinese)
[10] 杨颜铱,邓俊良,陈芸,等.精料水平和复合抗菌肽对川中黑山羊生长性能及血清中免疫球蛋白、补体、细胞因子和激素水平的影响[J].浙江农业学报,2017,29(8):1243-1252. YANG Y B,DENG J L,CHEN Y,et al.Effects of concentrate level and dietary supplementation of recombinant antimicrobial peptides(AMPs) on growth performance and serum immunoglobulin,cytokines,complement,hormone levels in male Chuanzhong Black goats[J].Acta Agriculturae Zhejiangensis,2017,29(8):1243-1252.(in Chinese)
[11] 蔡云川,赵书燕,林黑着,等.不同蛋白水平下添加抗菌肽对赤点石斑鱼影响的研究[J].上海海洋大学学报,2017,26(3):383-391. CAI Y C,ZHAO S Y,LIN H Z,et al.Effect of antibacterial peptides supplementation in different protein level diets on grouper(Epinephelus akaara)[J].Journal of Shanghai Ocean University,2017,26(3):383-391.(in Chinese)
[12] 董晓庆.抗菌肽对建鲤生长性能及免疫功能影响的研究[D].长春:吉林农业大学,2015. DONG X Q.Effect of antimicrobial peptides(AMPs) on growth performance and immune function in Jian Carp[D].Changchun:Jilin Agricultural University,2015.(in Chinese)
[13] 翟少伟,史庆超,陈学豪.饲料中添加抗菌肽Surfactin对吉富罗非鱼肠道健康的影响[J].水生生物学报,2016,40(4):823-829. ZHAN S W,SHI Q C,CHEN X H.Effect of dietary antimicrobial peptides Surfactin supplementation on parameters of intestinal health indices of genetically improved farmed tilapia[J].Acta Hydrobiologica Sinica,2016,40(4):823-829.(in Chinese)
[14] ZHOU X X,WANG Y B,LI W F.Effect of feeding apidaecin on common carp growth performances and immune function[J].Aquaculture,2008,279(1):108-112.
[15] 刘红柏,黄江,马爱枝,等.中草药复方添加剂对施氏鲟生长及血液生化指标的影响[J].水产学杂志,2009,22(3):14-18. LIU H B,HUANG J,MA A Z,et al.Effects of Chinese herb compound additives on growth and blood biochemical indicator of Amur sturgeon[J].Chinese Journal of Fisheries,2009,22(3):14-18.(in Chinese)
[16] 赵晶晶,陈成勋,王庆奎,等.黄柏对点带石斑鱼生理生化指标的影响[J].饲料工业,2012,33(6):15-19. ZHAO J J,CHEN C X,WANG Q K,et al.Effects of Phellodendron amurense on physiological and biochemical indexes of grouper[J].Feed Industry,2012,33(6):15-19.(in Chinese)
[17] 陈奕彬.抗菌肽对罗非鱼生长、抗氧化及免疫能力的影响[D].广州:华南农业大学,2016. CHEN Y B.Effects of antimicrobial peptides on growth performance,antioxidant activity and immunity of tilapia[D].Guangzhou:Huanan Agricultural University,2016.(in Chinese)
[18] 陈晓生,刘为民,周庆国,等.饲粮中添加抗菌肽对肉鸭血清代谢激素及生理生化指标的影响[J].兽药与饲料添加剂,2005,2:4-6. CHEN X S,LIU W M,ZHOU Q G,et al.Effects of antimicrobial peptides on metabolism hormone and physiological and biochemical indexes of duck blood[J].Veterinary Pharmaceuticals and Feed Additives,2005,2:4-6.(in Chinese)
[19] 惠天朝,施明华,朱荫湄.硒对罗非鱼慢性镉中毒肝抗氧化酶及转氨酶的影响[J].中国兽医学报,2000,20(3):264-266. HUI T C,SHI M H,ZHU Y M.Effects of selenium on antioxidant enzymes and transaminases of liver in cadmium chronic toxic tilapia nilotica[J].Chinese Journal of Veterinary Science,2000,20(3):264-266.(in Chinese)
[20] 王一娟,何义进,谢骏,等.抗菌肽对河蟹生长、免疫及抗氧化能力的影响[J].江苏农业科学,2011,39(2):340-343. WANG Y J,HE Y J,XIE J,et al.Effects of antimicrobial peptides on growth,immunity and antioxidant capacity of crab[J].Jiangsu Agricultural Sciences,2011,39(2):340-343.(in Chinese)
[21] 魏世娜,秦启伟.鱼类溶菌酶和组织蛋白酶研究进展[J].广西科学,2018,25(1):32-35. WEI S N,QIN Q W.Research progress of lysozyme and cathepsin in fish[J].Guangxi Sciences,2018,25(1):32-35.(in Chinese)
[22] KIRON V.Fish immune system and its nutritional modulation for preventive health care[J].Animal Feed Science and Technology,2012,173(1):111-133.
[23] 杨奇慧.不同水平维生素A对幼建鲤免疫功能的影响[D].成都:四川农业大学,2003. YANG Q H.Effect of different levels of vitamin A on immune function of juvenile Jian Carp[D].Chengdu:Sichuan Agricultural University,2003.(in Chinese)
[24] 王自蕊,谯仕彦,李波,等.饲料中添加天蚕素抗菌肽对湘云鲫生长性能、非特异性免疫功能及抗病力的影响[J].动物营养学报,2014,26(7):1856-1863. WANG Z R,QIAO S Y,LI B,et al.Effect on growth performance,nonspecific immunity and disease resistance of triploid crucian carp[J].Chinese Journal of Animal Nutrition,2014,26(7):1856-1863.(in Chinese)
[25] 毛盼,胡毅,李金龙,等.豆粕替代鱼粉对青鱼幼鱼生长及生理生化指标的影响[J].淡水渔业,2013,43(5):50-56. MAO P,HU Y,LI J L,et al.Effect of fish meal replacement by dietary soybean meal levels on growth and biochemical indices in juvenile mylopharvngodon piceus[J].Freshwater Fisheries,2013,43(5):50-56.(in Chinese)

Effects of Additive Amount of Antimicrobial Peptides on Biochemical Indices of Hybrid Sturgeon

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