China Animal Husbandry and Veterinary Medicine ›› 2020, Vol. 47 ›› Issue (5): 1472-1480.doi: 10.16431/j.cnki.1671-7236.2020.05.021

• Genetics and Breeding • Previous Articles     Next Articles

Identification of TGF-β Gene Family and Expression Analysis in Preimplantation Embryo Development of River Buffalo

PANG Chunying1, LIANG Shasha1, MA Xiaoya1, LU Xingrong1, DUAN Anqin1, DENG Tingxian1, HUANG Yunqi2, LIANG Xianwei1   

  1. 1. Guangxi Key Laboratory of Buffalo Genetics, Breeding and Reproduction Technology, Ministry of Agriculture and Rural Affairs, Guangxi Buffalo Research Institute, Chinese Academy of Agricultural Sciences, Nanning 530001, China;
    2. Shangdong Agricultural University, Tai'an 271000, China
  • Online:2020-05-20 Published:2020-05-18

Abstract: This study was aimed to identify the transforming growth factor-β (TGF-β) gene family in River buffalo,detect their expression profiling,and analyze the possible function of TGF-β gene in preimplantation embryo development.According to the reported TGF-β gene in human,TGF-β family members in River buffalo were identified at the whole genome level by BLASTP,the TGF-β gene information in Bos taurus,Capra hircus and Mus musculus were characterized.Phylogenetic tree of TGF-β gene family was constructed to elucidate evolutional relationship among these five species.The expression of TGF-β gene was calculated by Fragment of Per Kilobase of exon model per Million mapped reads (FPKM) based on RNA-seq data at four stages of preimplantation embryo development (2-cell,8-cell,morula and blastocyst).The results showed that there were 32,23,32 and 26 TGF-β gene in River buffalo,Bos taurus,Capra hircus and Mus musculus,respectively.The similar numbers of TGF-β gene among five species and uniform distribution in the phylogenetic tree indicated that the conservation of TGF-β gene distribution and function among different species.In 32 TGF-β gene of River buffalo,21 TGF-β gene did not express (or extremely low express) in preimplantation embryo development,6 TGF-β gene which had been identified as inhibitor,exhibited low expression level.5 TGF-β gene,which were involved in reproduction,showed high expression level,which suggested that only partial of TGF-β gene (5/32) rather than most of them (27/32) were involved in preimplantation embryo development of River buffalo.The results provided the foundation for the study of TGF-β gene family in River buffalo,and provided a theoretical basis for the functional analysis of TGF-β gene family in early embryonic development.

Key words: buffalo; preimplantation embryo development; TGF-β gene family; RNA-seq

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