猪干扰素-δ基因原核表达载体的构建及高效表达

doi:10.16431/j.cnki.1671-7236.2016.04.013

Abstract

Abstract: Interferon (IFN) had recieved much more attention because of its broad-spectrum antiviral, antitumor activity and immune regulation.One pair of primers were designed for amplifying pig IFN-δ gene with PCR method according to the relevant sequence from GenBank.The IFN-δ gene was cloned into prokaryotic expression vector, and the recombinant plasmid was obtained.We transformed the recombinant plasmid into E.coli Transetta BL21(DE3) strain and the protein expression was identified by SDS-PAGE and Western blotting.The results showed that pET-30a-DsbA-IFN-δ recombinant protein was 20 ku and expressed mainly in the form of inclusion body, these expressed protein could specific react with His-tag monoclonal antibody.

Key words: pig; interferon-δ; prokaryotic expression

CLC Number:

S852.2

LI Hong, LIU Cheng-qian, YAN Hua-xiang, WANG Jia-hao, YI Jian-zhong. Construction and High Level Expression of Prokaryotic Expression Vector of Pig Interferon-δ Gene[J]. , 2016, 43(4): 940-945.

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Construction and High Level Expression of Prokaryotic Expression Vector of Pig Interferon-δ Gene

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