新疆克拉玛依地区奶牛源致病性大肠杆菌的血清型、系统进化群及MLST分析

doi:10.16431/j.cnki.1671-7236.2021.04.029

Abstract

Abstract: The pathogenic Escherichia coli (E.coli) was isolated,identified,typed and analyzed for genetic evolution of 106 healthy adult Holstein cows in Karama,Xinjiang,and the epidemic situation of pathogenic Escherichia coli in cows in the investigation area was preliminarily known.With reference to the USDA detection method,106 stool samples were selectively enriched,and the E.coli was separated,purified and identified using MacConkey and Eosin Methylene Blue agar medium.The PCR method was used to identify 55 strains of E.coli.The isolates were subjected to pathogenic E.coli serotype identification,phylogenetic group and MLST type analysis,clustering and evolution studies were performed using goeBURST and Mega 7.0 biological software,and epidemiological analysis was performed.The results showed that the detection rate of Escherichia coli in fresh feces of 106 healthy Holstein cows was 51.89%.Nine serotypes related to pathogenicity were identified in 55 E.coli isolates,the detection rate was 54.55%.The detection rates of EPEC,EIEC and ETEC were 21.82%,18.81% and 14.55%,respectively.The dominant serotypes were O142∶K86 (B) and O124∶K72 (both 10.91%).The former belonged to EPEC,the latter belonged to EIEC,and the detection rate of EHEC serotype was 0.The phylogenetic group results show that the B1 group of 55 strains of E.coli accounted for the highest proportion,which was 70.91%,followed by group A,accounting for 21.82%,group D,which proportion was small,accounting for 7.27%,and the B2 group was not detected.MLST analysis showed that there were 24 ST types in the isolates,among which the dominant ST type was ST154 (18.18%).The evolutionary tree analysis revealed seven branches of evolution.In Conclusion,the feces of healthy dairy cows in Karamay,Xinjiang had a rich diversity of E.coli.There were not only pathogenic E.coli but also a certain evolutionary relationship between E.coli strains.Therefore,the excrement of healthy adult cows also had potential safety risk,so the detection and monitoring of pathogenic E.coli in cows excrement should be strengthened.

Key words: Escherichia coli from cows; serotype; phylogenetic group; multilocus sequence typing (MLST)

CLC Number:

S885.1

JIANG Wanlin, MA Xun, WANG Jing, PING Liying, CAI Siqi. Serotype,Phylogenetic Group and MLST Analysis of Pathogenic Escherichia coli Isolated from Dairy Cattle in Karamay,Xinjiang[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(4): 1414-1422.

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Serotype,Phylogenetic Group and MLST Analysis of Pathogenic Escherichia coli Isolated from Dairy Cattle in Karamay,Xinjiang

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