Abstract: To construct and express prokaryotic expression vector of bovine paraoxonase-1 and paraoxonase-2 (PON1 and PON2) and to obtained pure PON1 and PON2. Full length sequences of PON1 and PON2 genes were amplified from bovine liver by RT-PCR and were cloned into the expression vector-pET28a. PON1 and PON2 fusion proteins were expressed in BL21 under IPTG induction and the expressed fusion proteins were detected by SDSPAGE. The recombinant plasmids containing the target genes were constructed successfully. The fusion proteins were expressed in E coli in soluble form. The fusion proteins are expressed in soluble form, which is a useful reagent for further preparation of purifying and study of the function of PON1 and PON2 in blood vessel and diabetes mellitus, and boosting the birth weight of calves.

Key words: paraoxonases; PON1 gene; PON2 gene; clone; prokaryotic expression