›› 2008, Vol. 1 ›› Issue (9): 45-47.

• 生物技术 • Previous Articles     Next Articles

The Prevention of Blastomyces Albicans Contamination in Cell Culture

ZHAO Wei1,XUE Jianhua2   

  1. 1. Liaoning Medical University,Jinzhou 121001,China;2. Animal Epidemic Prevention and Inspection Station of Linghai City in Liaoning Province,Linghai 121200, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-09-20 Published:2008-09-20

Abstract: To study the feasibility of preventing blastomyces albicans contamination in cell culture by Fluconazole, Fluconazole was added into medium with different concentration,the survival of C2C12 was observed.The results showed that the combination of 300 μg/ml Fluconazole was better than others in preventing blastomyces albicans,and it did not obviously affect the survival of C2C12 cells.Conclusion: Fluconazole can be used to eliminate blastomyces albicans contamination in cell culture.Fluconazole, Amikacin and Cefradine can be used to prevent eumycete contamination in cell culture.

Key words: cell culture; blastomyces albicans; Fluconazole; eumycete contaminate

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