类器官的培养方法及应用前景

doi:10.16431/j.cnki.1671-7236.2023.07.010

Abstract

Abstract: In recent years, with the deepening of research in the field of in vitro modeling, it has been found that cell tests have some shortcomings, such as single cell, difficult to simulate complex tissue structure and normal physiological and pathological state of the body, and the high cost of animal tests and related physiological ethics and other issues, organoids have gradually become the focus of attention of scientists.Organoids are derived from the body's own tissues and stem cells, which are 3D structured cell clumps with original tissues and organs formed through 3D culture in vitro.They are highly consistent with the source tissues in terms of tissue structure, cell type and function.The construction of organoid model not only provides more methods for the research of replacing regenerated tissues and organs, but also plays an important role in tissue transplantation and repair.This review mainly introduces the culture methods for promoting organoid maturation, elaborates four methods, including microporous array method, microfluid method, bioreactor method and hydrogel matrix method, reviews the historical evolution of organoid culture system, and analyzes the limitations and future challenges in the field of organoid research in recent years.As well as the application prospect of organoids in emerging engineering technology, it aims to provide more theoretical basis and new ideas for further improvement of organoids culture methods and research and application in related fields.

Key words: organoids; cultural method; 3D cell culture; application status

CLC Number:

S858

WANG Manxi, LI Qinghao, SUN Juan, HU Hui, JIN Xin. Culture Methods and Application Prospects of Organoids[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(7): 2688-2696.

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Culture Methods and Application Prospects of Organoids

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[1]	LI Juanjuan, ZHANG Juntao, ZHAO Yingbo, HE Laizeng, ZHANG Fan, LI Yiping, YAN Penghui, HU Saina, LYU Yulin, DENG Lixin, ZHANG Zhiping. Research Progress and Application Prospect of Organoids [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 1985-1994.
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