南德温杂交肉牛生长分化因子10基因多态性与体尺性状的关联分析

Abstract

Abstract: Blood samples were randomly selected from genomic DNA of 98 South Devon hybrid progeny to construct DNA pool, and sequence variations and polymorphism of growth differentiation factor 10 (GDF10) gene were analyzed by high resolution melting curve (HRM) analysis techniques and direct sequencing.SHEsis and PHASE were used to screen the SNPs in GDF10 gene by the wave height of sequencing map.The association between genotypes and body measurement was evaluated by SPSS 17.0.The results showed that three SNPs 1113 (G/A), 9569 (G/A) and 9628 (G/A) of GDF10 gene were identified in the population.Analysis of association of polymorphism with body measurement traits at all mutation loci showed that there were significant effects on body height, body length, chest circumference and cannon bone girth (P<0.05).There were 8 haplotype combinations in the cattle populations, in them AAA and GGA had significant effects on body height, body length, chest circumference and cannon bone girth (P<0.05).The results suggested that three SNPs and two haplotype combinations might have potential effects on production traits in the above mentioned cattle populations and could be used for marker-assisted selection.

Key words: South Devon hybrid progeny; GDF10 gene; polymorphism; haplotypes; body measurement traits

CLC Number:

S823

GUO Xian, LI Tian-ke, PEI Jie, BAO Peng-jia, QIN Wen, YAN Ping. GDF10 Gene Polymorphism and its Association with Body Measurement Traits in South Devon Hybrid Progeny[J]. , 2014, 41(12): 197-203.

References

1 马利军,赵益元,蒋德元,等.南德温肉牛改良四川宣汉牛效果调查报告[J].黄牛杂志,2002,28(2):51～52.
2 扎西卓玛,郭宪,阎萍,等.高分辨率熔解曲线分析法检测牦牛脂蛋白脂酶外显子7的多态性及与生长性状相关性分析[J].中国畜牧兽医,2013,40(7):84～88.
3 李天科,梁春年,郎侠,等.甘南牦牛GDF-10基因多态与生产性状的相关性分析[J].中国农业科学,2014,47(1):161～169.
4 李爱民,马云,杨东英,等.鲁西牛ANGPTL6基因的3个多态位点与其生长性状的关联性分析[J].中国农业科学,2012,45(11):2306～2314.
5 陈喜英.南德温肉牛改良丘陵山区本地牛效果的调查[J].河南农业,2009(2):47.
6 陈幼春.现代肉牛生产[M].第2版.北京:中国农业出版社,2012.
7 Adoligbe C,Zan L,Farougou S,et al.Bovine GDF10 gene polymorphism analysis and its association with body measurement traits in Chinese indigenous cattle[J].Mol Biol Rep,2012,39(4):4067～4075.
8 Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].J Hum Genet,1980,32(3):314～331.
9 Chu M X,Lu L,Feng T,et al.Polymorphism of bone morphogenetic protein 4 gene and its relationship with litter size of Jining Grey goats[J].Mol Biol Rep,2010,38(7):4315～4320.
10 Feng T,Geng C X,Lang X Z,et al.Polymorphisms of caprine GDF9 gene and their association with litter size in Jining Grey goats[J].Mol Biol Rep,2011,38(8):5189～5197.
11 Gundry C N,Dobrowolski S F,Martin Y R,et al.Base-pair neutral homozygotes can be discriminated by calibrated high-resolution melting of small amplicons[J].Nucleic Acids Res,2008,36(10):3401～3408.
12 Hino J,Takao M,Takeshita N,et al.cDNA cloning and genomic structure of human bone morphogenetic protein-3b (BMP-3b)[J].Biochem Biophys Res Commun,1996,223(2):304～310.
13 Hino J,Matsuo H,Kangawa K.Bone morphogenetic protein-3b (BMP-3b) gene expression is correlated with differentiation in rat calvarial osteoblasts[J].Biochem Biophys Res Commun,1999,256(2):419～424.
14 Hino J,Nishimatsu S,Nagai T,et al.Coordination of BMP-3b and cerberus is required for head formation of Xenopus embryos[J].Dev Biol,2003,260(1):138～157.
15 Hino J,Kangawa K,Matsuo H,et al.Bone morphogenetic protein-3 family members and their biological functions[J].Front Biosci,2004,9:1520～1529.
16 Kingsley D M.The TGF-beta superfamily:New members,new receptors,and new genetic tests of function in different organisms[J].Genes Dev,1994,8(2):133～146.
17 Liu Y F,Zan L S,Li K,et al.A novel polymorphism of GDF5 gene and its association with body measurement traits in Bos taurus and Bos indicus breeds[J].Mol Biol Rep,2010,37(1):429～434.
18 Nei M,Li W H.Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76(10):5269～5273.
19 Ye M H,Chen J L,Zhao G P,et al.Associations of A-FABP and H-FABP markers with the content of intramuscular fat in Beijing-You chicken[J].Anim Biotechnol,2010,21(1):14～24.
20 Zhao R B,Lawler A M,Lee S J.Characterization of GDF-10 expression patterns and null mice[J].Dev Biol,1999,212:68～79.

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GDF10 Gene Polymorphism and its Association with Body Measurement Traits in South Devon Hybrid Progeny

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