China Animal Husbandry and Veterinary Medicine ›› 2024, Vol. 51 ›› Issue (2): 601-613.doi: 10.16431/j.cnki.1671-7236.2024.02.017

• Genetics and Breeding • Previous Articles     Next Articles

Genetic Diversity and Origin Evolution Analysis of Qingyang Donkey Based on mtDNA D-loop Region Sequence

SHI Haina1, WANG Yongjie2, LIANG Wanpeng1, GENG Zhiguang1, LI Shien1, XU Zhenfei1, LIU Gang3, LIU Zhe4   

  1. 1. Qingyang Academy of Agricultural Sciences, Qingyang 745000, China;
    2. Ningxia Fisheries Research Institute, Yinchuan 750001, China;
    3. National Animal Husbandry Service, Beijing 100193, China;
    4. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2023-06-07 Online:2024-02-05 Published:2024-01-29
  • Contact: 农业农村部"物种品种资源保护"(2130135);科技部"家养动物种质资源平台"之"庆阳驴遗传材料制作与保存"(XXZZ182019254);甘肃马驴种质资源评价利用及高效养殖技术研究与示范(GARS-TSYZ-2)
  • Supported by:
    The project was supported by the National Key Research and Development Program of China (2019YFC1905301);National Natural Science Foundation of China (22078115,21776108,21690083,22008078).

Abstract: 【Objective】 This study was aimed to explore the genetic diversity and maternal origin of Qingyang donkey breeding population, understand its genetic information, and provide theoretical basis for protecting germplasm resources, breeding, and genetic improvement in Qingyang donkey.【Method】 133 Qingyang donkeys were randomly selected, the mitochondrial DNA (mtDNA) D-loop region sequences were analyzed by PCR amplification, sequencing and alignment, and the genetic diversity and maternal origin of Qingyang donkey were explored.【Result】 520 bp D-loop base sequence were obtained, the AT content (57.3%) was higher than GC content (42.8%), indicating there was bias in base composition.There were 38 SNPs of D-loop region, including 8 conversions.The nucleotide diversity (Pi), haplotype diversity (Hd) and average nucleotide difference (K) were 0.01591, 0.895 and 8.274, respectively, which was lower than the average of European and Chinese domestic donkey studies, indicating that the nucleotide variation of Qingyang donkey breed was relatively poor.The genetic distances of 35 haplotypes of D-loop region in Qingyang donkey ranged from 0.002 to 0.042.Phylogenetic results showed that there were two mitochondrial lineages, indicating that there were two mitochondrial maternal origins, and the genetic distance between Qingyang donkey and Croatian domestic donkey was relatively close.【Conclusion】 The experiment preliminarily revealed the nucleotide variation of Qingyang donkey was poor from the molecular level, the degree of hybridization was high, and the mtDNA genetic polymorphism was gradually lost, so the protection of genetic resources of Qingyang donkey breed should be strengthened.

Key words: Qingyang donkey; mtDNA; D-loop region; genetic diversity; origin

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