China Animal Husbandry and Veterinary Medicine ›› 2023, Vol. 50 ›› Issue (10): 4125-4132.doi: 10.16431/j.cnki.1671-7236.2023.10.026

• Genetics and Breeding • Previous Articles     Next Articles

Application Progress on Whole Genome Resequencing in Farm Animals

YE Wen, SUN Dongxiao, HAN Bo   

  1. State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
  • Received:2023-03-03 Online:2023-10-05 Published:2023-09-26

Abstract: Whole genome resequencing (WGRS) is the whole genome level sequencing between different individuals of species with known reference genome sequences,which has the advantages of abundant detection variant types,high cost performance and wide application.With the reduction of sequencing cost and the completion of animal genome sequencing,WGRS technology has become an important tool for the research of genetic variation in animals.The large amount of genetic variation information obtained through WGRS,including single nucleotide polymorphism (SNP),insertion/deletion (InDel),structural variation (SV) and copy number variation (CNV),etc.,enriches the existing genome sequence.The resulting large-scale dataset of WGRS provides a genomic information base for exploring phenotypic traits and genetic improvement in animals,and promotes the in-depth study and utilization of the genetic resources.In this article,the condition of WGRS technology and its key influencing factors (sequencing depth,sequence comparison and variant detection) were summarized,the application progress on this technology in the research field of important farm animals (cattle,sheep,pigs and chickens) was reviewed,and the future research trend focusing on integrated analysis of resequencing data,accurate phenotypic records and multi-omics information was prospected.

Key words: whole genome resequencing; farm animals; genetic diversity; selection and evolution; functional gene

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