长白山中华蜜蜂遗传多样性分析

doi:10.16431/j.cnki.1671-7236.2023.12.021

Abstract

Abstract: 【Objective】 This study was aimed to explore the genetic diversity of Changbaishan Chinese bee,and provide theoretical basis for its conservation and utilization.【Method】 A total of 195 broods of Changbaishan Chinese bee were collected from 7 sampling sites in the national Mesobee (Changbaishan Chinese bee) conservation area.11 microsatellite markers were used for genotyping,and genetic parameters such as polymorphism information content (PIC),expected heterozygosity (He),observed heterozygosity (Ho),inbred coefficient (Fis),differentiation coefficient (Fst) and interpopulation gene flow (Nm) were evaluated to study the genetic diversity of each population,and construct phylogenetic tree.【Result】 A total of 103 alleles were detected among 195 Changbaishan Chinese bee.Except AC306,AG005C,A028,AP049 and AP313,the other 6 microsatellite loci showed high polymorphism.The average polymorphism information content, expected heterozygosity and observed heterozygosity were 0.5080,0.5467 and 0.4662,respectively.The average inbred coefficient of Changbaishan Chinese bee was -0.0107.The differentiation coefficient of the 7 populations ranged from 0.0217 (Changbai and Fusong) to 0.3389 (Antu and Huinan).The gene flow between populations ranged from 0.7126 to 0.9785.The phylogenetic tree showed that there were three different evolutionary branches of the Changbaishan Chinese bee.【Conclusion】 The overall genetic polymorphism of Changbaishan Chinese bee was high,and the genetic diversity of different sampling sites was different.The results had important guiding significance for the future protection and utilization of Changbaishan Chinese bee.

Key words: Changbaishan Chinese bee; microsatellites; genetic differentiation; genetic diversity

CLC Number:

S893.2

ZHUANG Mingliang, LI Jianfei, GE Peng, WANG Qi, WANG Zhi, QI Jingwen, NIU Qingsheng, LI Zhiyong. Genetic Diversity Study of Changbaishan Chinese Bee[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4992-4999.

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Genetic Diversity Study of Changbaishan Chinese Bee

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