浙江6个地方猪种的mtDNA控制区多态性分析

doi:10.16431/j.cnki.1671-7236.2015.02.033

Abstract

Abstract: This study analyzed one 116 mitochondrial DNA sequences from Chun'an spotted pigs and other five domestic pig breeds in Zhejiang province.The referenced pig breeds were Landrace and Wannan spotted pigs.The results showed that the 581 bp mtDNA control region sequence of Zhejiang domestic pig breeds included 14 variable sites.The nucleotide diversity was 0.00403±0.00036 and the haplotype diversity was 0.835±0.018.There were 15 haplotypes which from Hap_5 to Hap_19 in domestic pigs,and Landrace haplotypes were from Hap_1 to Hap_4.The highest haplotype frequency in Chun'an spotted pigs and Wannan spotted pigs was Hap_8 (0.680 and 0.833).Hap_5 frequency was highest in Jiaxing Black pigs (0.625), and in Shengxian spotted pigs and Bihu pigs that was Hap_6 (0.500 and 0.375).Hap_5 and Hap_8 frequencys were high mainly in Chalu Black pigs (0.429 and 0.429).Jinhua pigs had eleven haplotypes and the highest frequency was Hap_5 (0.241).A Neighbor-Joining phylogeny tree revealed 5 clades which signed from A to E among 6 Zhejiang domestic pig breeds.Chun'an spotted pigs belonged to E clade mainly,and the samples percent in Chun'an spotted pigs was 68.0% (17/25).C clade were all Jinhua pigs,and the samples percent in Jinhua pigs was 29.3% (17/58).Meanwhile,the percent of Jinhua pigs in D clade was 24.1% (14/58).It was concluded that the mtDNA control region polymorphism in Zhejiang domestic pig breeds were rich relatively and its mutations of base were different obviously from Landrace.There was close relationship between haplotype composition and frequency of domestic pigs and their geographic distribution and breed characteristics.

Key words: domestic pig; Chun'an spotted pig; mitochondrial DNA; polymorphism

CLC Number:

S813.3

ZHANG Xue-dong, LI Qing-hai, TONG Xun-quan, WANG Huan-huan, LOU Li-feng. Analysis on Mitochondrial DNA Control Region Polymorphism in Six Domestic Pig Breeds in Zhejiang Province[J]. , 2015, 42(2): 443-451.

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Analysis on Mitochondrial DNA Control Region Polymorphism in Six Domestic Pig Breeds in Zhejiang Province

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