牛肉源大肠杆菌的耐药性检测及相关耐药基因分布

doi:10.16431/j.cnki.1671-7236.2015.02.034

Abstract

Abstract: To investigate the antibiotic resistance and resistance genes in Escherichia coli (E.coli) isolated from beef,susceptibilities to 11 antibiotics were conducted on 117 isolates,antibiotic resistance determinants were detected by using conventional and/or multiple PCR.The results showed that resistance rates of 117 isolates to tetracycline,ampicillin,streptomycin,sulfisoxazole were 89%,42%,38%,22%,respectively.As revealed by detecting resistance determinants,tet(A),bla_TEM1,strA-strB,sul2 were the most prevalent genes among tetracycline-resistant isolates,ampicillin-resistant isolates,streptomycin-resistant isolates and sulfisoxazole-resistant isolates,respectively.The detection rates of tet(A),bla_TEM1,strA-strB and sul2 were 55%,73%,38% and 77%,respectively.The conclusion was that E.coli isolated from beef was seriously resistant to antibiotics,and antibiotics administration in beef cattle industry promoted the emergence and the propagation of antibiotic resistance in E.coli.Data reported here clearly emphasized the need for a stricter application of antibiotics restriction policies in feedlot setting.

Key words: Escherichia coli; antibiotic resistance; resistance determinants; beef cattle

CLC Number:

S859.7

WU Rui-bing, GAO Yu-min, WANG Peng-xiang, YANG Zhong, ZHANG Jian-yu, WANG Hai-sheng. Detection of Antibiotic Resistance and Distribution of Resistance Determinants in Escherichia coli Isolated from Beef[J]. , 2015, 42(2): 452-458.

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Detection of Antibiotic Resistance and Distribution of Resistance Determinants in Escherichia coli Isolated from Beef

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