猪瘟病毒非结构蛋白功能的研究进展

摘要/Abstract

摘要： 近年来,人们对猪瘟病毒(classical swine fever virus,CSFV)的结构蛋白研究比较透彻,而对非结构蛋白功能的研究相对较少。非结构蛋白对病毒的复制、致病性及对宿主细胞功能的调节均发挥重要作用,这些研究成果对于揭示CSFV的致病机理和新型疫苗的开发具有十分重要的意义。作者全面综述了CSFV非结构蛋白的生物学功能,以期为研究者提供参考。

关键词: 猪瘟病毒; 非结构蛋白; 功能

Abstract: In recent years,the function of these structural proteins of classical swine fever virus (CSFV) was extensivly expored, the CSFV encoded non-structural proteins had also been studied, and the results showed that these proteins played important roles in the replication, virulence and regulation of the host cell function, there were still many unknow points needed to be investigated. This paper reviewed research progress on the function of the non-structural proteins of CSFV, which might provide references for researchers.

Key words: classical swine fever virus; non-structural protein; function

中图分类号:

S852.65⁺1

陈果亮, 余兴龙. 猪瘟病毒非结构蛋白功能的研究进展[J]. 中国畜牧兽医, 2013, 40(8): 185-189.

CHEN Guo-liang, YU Xing-long. Research Progress on Funtion of Non-structural Proteins of Classical Swine Fever Virus[J]. , 2013, 40(8): 185-189.

参考文献

1 Armengol E, Wiesmuller K H, Wienhold D, et al. Identification of T-cell epitopes in the structural and non-structural proteins of classical swine fever virus[J]. J Gen Virol, 2002, 83(3):551~560.

2 Bauhofer O, Summerfield A, McCullough K C, et al. Role of double-stranded RNA and N^pro of classical swine fever virus in the activation of monocyte-derived dendritic cells[J]. Virology, 2005, 343(1):93~105.

3 Bauhofer O, Summerfield A, Sakoda Y, et al. Classical swine fever virus Npro interacts with interferon regulatory factor 3 and induces its proteasomal degradation[J]. J Virol, 2007, 81(7):3087~3096.

4 Doceul V, Charleston B, Crooke H, et al.The N^pro product of classical swine fever virus interacts with IkappaBalpha, the NF-kappaB inhibitor[J]. J Gen Virol, 2008, 89(8):1881~1889.

5 Fernandez-Sainz I, Gladue D P, Holinka L G, et al. Mutations in classical swine fever virus NS4B affect virulence in swine[J]. J Virol, 2010, 84(3):1536~1549.

6 Gallei A, Blome S, Gilgenbach S, et al. Cytopathogenicity of classical swine fever virus correlates with attenuation in the natural host[J]. J Virol, 2008, 82(19):9717~9729.

7 Gladue D P, Gavrilov B K, Holinka L G, et al. Identification of an NTPase motif in classical swine fever virus NS4B protein[J]. Virology, 2011, 411(1):41~49.

8 Gladue D P, Holinka L G, Largo E, et al. Classical swine fever virus p7 protein is a viroporin involved in virulence in swine[J]. J Virol, 2012, 86(12):6778~6791.

9 Guo K K, Tang Q H, Zhang Y M, et al. Identification of two internal signal peptide sequences: Critical for classical swine fever virus non-structural protein 2 to trans-localize to the endoplasmic reticulum[J]. Virol J, 2011, 8:236.

10 He L, Zhang Y M, Lin Z, et al. Classical swine fever virus NS5A protein localizes to endoplasmic reticulum and induces oxidative stress in vascular endothelial cells[J]. Virus Genes, 2012, 45(2):274~282.

11 Kang K, Guo K, Tang Q, et al. Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis[J]. Mol Biol Rep, 2012, 39(12): 10515~10524.

12 La Rocca S A, Herbert R J, Crooke H, et al. Loss of interferon regulatory factor 3 in cells infected with classical swine fever virus involves the N-terminal protease, N^pro[J]. J Virol, 2005, 79 (11): 7239~7247.

13 Monso M, Tarradas J, de la Torre B G, et al. Peptide vaccine candidates against classical swine fever virus: T cell and neutralizing antibody responses of dendrimers displaying E2 and NS2-3 epitopes[J]. J Pept Sci, 2011, 17(1):24~31.

14 Moulin H R, Seuberlich T, Bauhofer O, et al. Nonstructural proteins NS2-3 and NS4A of classical swine fever virus: Essential features for infectious particle formation[J]. Virology, 2007, 365(2): 376~389.

15 Rau H, Revets H, Balmelli C, et al. Immunological properties of recombinant classical swine fever virus NS3 protein in vitro and in vivo[J]. Vet Res, 2006, 37(1): 155~168.

16 Ruggli N, Summerfield A, Fiebach A R, et al. Classical swine fever virus can remain virulent after specific elimination of the interferon regulatory factor 3-degrading function of N^pro[J]. J Virol, 2009, 83(2):817~829.

17 Seago J, Hilton L, Reid E, et al. The N^pro product of classical swine fever virus and bovine viral diarrhea virus uses a conserved mechanism to target interferon regulatory factor-3[J]. J Gen Virol, 2007, 88(11):3002~3006.

18 Seago J, Goodbourn S, Charleston B.The classical swine fever virus N^pro product is degraded by cellular proteasomes in a manner that does not require interaction with interferon regulatory factor 3[J]. J Gen Virol, 2010, 91(3):721~726.

19 Sheng C, Xiao M, Geng X, et al. Characterization of interaction of classical swine fever virus NS3 helicase with 3' untranslated region[J]. Virus Res, 2007, 129(1):43~53.

20 Sheng C, Chen Y, Xiao J, et al. Classical swine fever virus NS5A protein interacts with 3'-untranslated region and regulates viral RNA synthesis[J]. Virus Res, 2012a, 163(2):636~643.

21 Sheng C, Wang J, Xiao J, et al. Classical swine fever virus NS5B protein suppresses the inhibitory effect of NS5A on viral translation by binding to NS5A[J]. J Gen Virol, 2012b, 93(5):939~950.

22 Tamura T, Sakoda Y, Yoshino F, et al. Selection of classical swine fever virus with enhanced pathogenicity reveals synergistic virulence determinants in E2 and NS4B[J]. J Virol, 2012, 86(16):8602~8613.

23 Tang Q H, Zhang Y M, Fan L, et al. Classic swine fever virus NS2 protein leads to the induction of cell cycle arrest at S-phase and endoplasmic reticulum stress[J]. Virology Journal, 2010, 7:4.

24 Tang Q, Guo K, Kang K, et al. Classical swine fever virus NS2 protein promotes interleukin-8 expression and inhibits MG132-induced apoptosis[J]. Virus Genes, 2011, 42(3):355~362.

25 Tratschin J D, Moser C, Ruggli N, et al. Classical swine fever virus leader proteinase N^pro is not required for viral replication in cell culture[J]. J Virol, 1998, 72(9):7681~7684.

26 Voigt H, Wienhold D, Marquardt C, et al. Immunity against NS3 protein of classical swine fever virus does not protect against lethal challenge infection[J]. Viral Immunol, 2007, 20(3):487~494.

27 Wang P, Wang Y, Zhao Y, et al. Classical swine fever virus NS3 enhances RNA-dependent RNA polymerase activity by binding to NS5B[J]. Virus Res, 2010, 148(1~2):17~23.

28 Wang Y, Zhu Z, Wang P, et al. Characterisation of interaction between NS3 and NS5B protein of classical swine fever virus by deletion of terminal sequences of NS5B[J]. Virus Res, 2011, 156(1~2):98~106.

29 Wen G, Chen C, Luo X, et al. Identification and characterization of the NTPase activity of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) expressed in bacteria[J]. Arch Virol, 2007, 152(8):1565~1573.

30 Xiao M, Zhang C Y, Pan Z S, et al. Classical swine fever virus NS5B-GFP fusion protein possesses an RNA-dependent RNA polymerase activity[J]. Arch Virol, 2002, 147(9):1779~1787.

31 Xiao M, Chen J, Li B.RNA-dependent RNA polymerase activity of classical swine fever virus NS5B protein expressed in natural host cells[J]. Acta Virol, 2003, 47(2):79~85.

32 Xiao M, Gao J, Wang W, et al. Specific interaction between the classical swine fever virus NS5B protein and the viral genome[J]. Eur J Biochem, 2004, 271(19):3888~3896.

33 Xiao M, Wang Y, Zhu Z, et al.Influence of NS5A protein of classical swine fever virus (CSFV) on CSFV internal ribosome entry site-dependent translation[J]. J Gen Virol, 2009, 90(12):2923~2928.

34 Xu H, Hong H X, Zhang Y M, et al. Cytopathic effect of classical swine fever virus NS3 protein on PK-15 cells[J]. Intervirology, 2007, 50(6):433~438.

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