1株贝莱斯芽孢杆菌G02全基因组测序分析

doi:10.16431/j.cnki.1671-7236.2025.05.004

摘要/Abstract

摘要： 【目的】对贝莱斯芽孢杆菌(Bacillus velezensis)G02进行全基因组序列分析，挖掘其抑菌和抗菌特性相关基因，以期详细解析其基因组信息。【方法】采用二代测序技术，通过Illumina NovaSeq 6000平台对贝莱斯芽孢杆菌G02进行全基因测序，通过基因组装、基因组全长、功能注释及次级代谢产物预测分析其基因组特征。【结果】全基因组测序结果显示，贝莱斯芽孢杆菌G02基因组全长为3 891 653 bp，共有3 749个蛋白质编码基因，GC含量为46%；基因组序列由12个contigs组成，存在92个非编码RNA，包括8个5S rRNA、2个16S rRNA、1个23S rRNA、80个tRNA和1个tmRNA。功能注释结果显示，GO数据库中注释到9个与抗氧化活性相关的基因；KEGG数据库注释到31条通路，其中参与细菌素合成和免疫相关通路的基因有ko00253、ko00261、ko00521等。通过antiSMASH在线软件预测，共注释到12个次级代谢产物合成基因簇。【结论】贝莱斯芽孢杆菌G02是潜在的产抑菌活性物质的菌株，研究结果为探究贝莱斯芽孢杆菌G02的抑菌机理提供了基础。

关键词: 芽孢杆菌; 全基因组测序; 次级代谢产物; 功能注释

Abstract: 【Objective】 The whole genome sequence analysis of Bacillus velezensis G02 was carried out to explore the genes related to its antibacterial and bacteriogenic properties in order to analyze its genome information in detail.【Method】 The whole gene of Bacillus velezensis G02 was sequenced by Illumina NovaSeq 6000 platform using next-generation sequencing technology.Genome characteristics were analyzed by gene assembly,genome length,functional annotation and secondary metabolite prediction.【Result】 The whole genome sequencing results showed that the total length of Bacillus velezensis G02 genome was 3 891 653 bp,with 3 749 protein-coding genes and a GC content of 46%.The genome sequence consists of 12 contigs with 92 non-coding RNA,including 8 5S rRNA,2 16S rRNA,1 23S rRNA,80 trRNA and 1 tmRNA.Functional annotation results showed that 9 genes related to antioxidant activity were annotated in GO database.KEGG database annotated 31 pathways,among wihch the genes involved in bacterin synthesis and immune-related pathways were ko00253,ko00261 and ko00521,etc.A total of 12 secondary metabolite synthesis gene clusters were annotated by antiSMASH online prediction software.【Conclusion】 Bacillus velezensis G02 was a potential strain producing antibacterial active substances,and the results provided a basis for the development and exploration of the antibacterial mechanism of Bacillus velezensis G02.

Key words: Bacillus; whole genome sequencing; secondary metabolites; function annotation

中图分类号:

S852.61⁺6

余梦博, 马浩天, 李杨, 潘瑞雪, 龙赟而, 赵一宁, 马驿, 周淑贞. 1株贝莱斯芽孢杆菌G02全基因组测序分析[J]. 中国畜牧兽医, 2025, 52(5): 1977-1986.

YU Mengbo, MA Haotian, LI Yang, PAN Ruixue, LONG Yuner, ZHAO Yining, MA Yi, ZHOU Shuzhen. Whole Genome Sequencing Analysis of a Strain of Bacillus velezensis G02[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(5): 1977-1986.

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