1株猪源动物联合乳杆菌S7全基因组测序及生物信息学分析

doi:10.16431/j.cnki.1671-7236.2025.01.003

摘要/Abstract

摘要： 【目的】旨在对1株具有较强耐酸耐胆盐特性的猪源动物联合乳杆菌S7进行全基因组测序及生物信息学分析。【方法】结合三代PacBio RS Ⅱ和二代Illumina HiSeq 2000测序技术对动物联合乳杆菌S7进行了全基因组测序,在此基础上使用GO、EggNOG、KEGG、CAZy、VFDB和CARD等数据库注释功能基因,利用TYGS(Type Strain Genome Server)构建系统发育树,并与2株同源模式菌株进行了共线性分析。【结果】动物联合乳杆菌S7的基因组大小为2.03 Mb,GC含量为44.14%,共预测到1 993个编码基因,包含65个tRNA、19个rRNA、35个sRNA、29个持家基因、11个基因组岛、6个前噬菌体、4个CRISPR-Cas、8个插入序列和10个转座子;分别有1 521、1 567、1 185和60个基因在GO、EggNOG、KEGG和CAZy数据库中得到注释;在VFDB和CARD数据库中注释到181个毒力基因和110个耐药基因;另外有14个基因参与耐酸,2个基因参与耐胆盐,22个基因参与黏附和聚集;基因组中还包括与温度应激、氧化应激和细菌素合成等多种与益生功能相关的基因。系统发育树和共线性分析发现,该菌株与模式菌株Ligilactobacillus animalis P38的进化关系最近。【结论】动物联合乳杆菌S7基因组中具有与耐酸、耐胆盐、黏附和聚集、温度应激、氧化应激和细菌素等相关的功能基因,可为菌株在饲料添加剂中的科学应用提供理论依据。

关键词: 猪; 动物联合乳杆菌; 全基因组测序; 基因注释

Abstract: 【Objective】 The aim of this study was to sequence the whole genome and analyze the bioinformatics of Ligilactobacillus animalis S7,a strain isolated from pig with good acid tolerance and bile salt tolerance. 【Method】 The sequencing technology combining the third generation PacBio RS Ⅱ sequencing platform and the second generation Illumina HiSeq 2000 sequencing platform was used to sequence the genome of S7,and then the functional genes of the genome were annotated in the databases of GO,EggNOG,KEGG,CAZy,VFDB and CARD.The phylogenetic tree was constructed used TYGS and collinearity analysis was performed with two nearest type strains. 【Result】 The genome size of Ligilactobacillus animalis S7 was 2.03 Mb,and the GC content was 44.14%.A total of 1 993 encoding genes were predicted,including 65 tRNA,19 rRNA,35 sRNA,29 housekeeping genes,11 gene isolands,6 prophages,4 CRISPR-Cas,8 insertion sequences and 10 transposons.1 521,1 567,1 185,and 60 genes were annotated in the databases of GO,EggNOG,KEGG and CAZy,respectively.181 virulence genes and 110 drug resistance genes were annotated in VFDB and CARD databases.In addition,14 acid tolerance-related genes,2 bile salt tolerance-related gene,22 cell adhesion and aggregation-related genes were found in S7.A variety of genes related to heat stress,oxidative stress resistance,and bacteriocin synthesis that are associated with probiotic functions were also found in the genome of S7.The phylogenetic tree and collinearity analysis results indicated that the strain had the nearest evolutionary relationship to type strain Ligilactobacillus animalis P38. 【Conclusion】 The genome of Ligilactobacillus animalis S7 had functional genes related to acid tolerance,bile salt tolerance,adhesion and aggregation,temperature stress,oxidative stress,and bacteriocin,which provided theoretical basis for the scientific application of the strain in feed additives.

Key words: pig; Ligilactobacillus animalis; whole genome sequencing; gene annotation

中图分类号:

S852.6

刘辉, 季海峰, 王四新, 陈美霞, 张董燕. 1株猪源动物联合乳杆菌S7全基因组测序及生物信息学分析[J]. 中国畜牧兽医, 2025, 52(1): 25-38.

LIU Hui, JI Haifeng, WANG Sixin, CHEN Meixia, ZHANG Dongyan. Whole Genome Sequencing and Bioinformatics Analysis of Ligilactobacillus animalis S7 from Pig[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(1): 25-38.

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