板青颗粒对靶动物猪的安全性试验

doi:10.16431/j.cnki.1671-7236.2024.09.043

摘要/Abstract

摘要： 【目的】评估板青颗粒对猪的安全性，为临床合理用药提供参考依据。【方法】选择32头110日龄左右健康猪，随机分为4组，板青颗粒1倍(100 mg/kg BW)、3倍(300 mg/kg BW)、5倍(500 mg/kg BW)剂量组和空白对照组，每组8头。试验组饮水给药14 d，停药后继续观察10 d；空白对照组正常饲养，不给药。每天上午、下午逐头观察并记录试验猪的临床表现，在给药前(试验第0天，D0)、给药结束(试验第15天，D15)、观察结束(试验第25天，D25)称重，并在空腹称重后采血，检测血液常规指标和生化指标。分别在给药结束和观察结束时对5倍剂量组和空白对照组猪进行组织病理学检查。【结果】试验期间各剂量组试验猪精神状态、食欲、二便均表现正常，生长性能指标差异均不显著(P＞0.05)；血液常规指标检测结果显示，试验猪红细胞数量、血红蛋白含量两个指标均随着给药剂量的增加而升高，但均在正常范围内波动，其余个别指标虽具有显著差异但不具有剂量相关性，且指标数值均在正常范围内。血液生化指标检测结果显示，试验猪血清总蛋白随着给药浓度的增加而升高，血糖含量随着给药浓度的升高而降低，但均在正常范围内波动，其余个别指标虽具有显著差异但不具有剂量相关性，且指标数值均在历史资料的参考范围内。组织病理学观察结果显示，5倍剂量组试验猪的心脏、肝脏、脾脏、肺脏、肾脏各组织器官结构清晰，层次分明，发育正常，未发现与中药制剂作用有关的细胞变性、坏死及炎性细胞浸润等病理性变化，各组织与空白对照组相比均未见明显的差异。【结论】板青颗粒5倍剂量(500 mg/kg BW)对猪用药安全。

关键词: 板青颗粒; 猪; 血液常规指标; 血液生化指标; 安全性

Abstract: 【Objective】 This experiment was aimed to evaluate the safety of Banqing granules on pigs, and provide reference for clinical rational drug use. 【Method】 32 healthy pigs of about 110 days of age were selected and randomly divided into recommended dose (100 mg/kg BW), triple dose (300 mg/kg BW), fivefold dose (500 mg/kg BW) groups of Banqing granules, and control group, with 8 pigs in each group.Pigs in experimental groups were administered in drinking water for 14 days and continued observation for 10 days after withdrawal.Pigs in control group were raised normally without medication.Clinical manifestations of experimental pigs were observed and recorded one by one in the morning and afternoon every day.The pigs were weighed before administration (experiment day 0, D0), after administration (experiment day 15, D15) and after observation (experiment day 25, D25), and blood was collected after fasting weighing to detect blood routine and blood biochemical indexes.Histopathological examinations were performed on animals in fivefold dose group and control group after administration and after observation, respectively. 【Result】 During the experiment, the mental state, appetite and bowel movements of pigs in each dose group were normal, and the growth performance indexes were not significantly different (P＞0.05).Blood routine index results showed that the number of red blood cells and hemoglobin content of pigs increased with the increase of dose, but both fluctuated within the normal range, and the other individual indicators had significant differences but no dose correlation and the index values were within the normal range.Blood biochemical index results showed that serum total protein of pigs increased with the increase of administration concentration, blood glucose content of pigs decreased with the increase of administration concentration but all fluctuated within the normal range, and other individual indexes showed significant differences but no dose correlation and the index values were all within the reference range of historical data.The histopathological results showed that the heart, liver, spleen, lung and kidney of pigs in fivefold dose group had clear structure, distinct layers and normal development, and no pathological changes related to the action of Chinese medicine were found, such as cell degeneration, necrosis and inflammatory cell infiltration, and no significant differences were found between the tissues and control group. 【Conclusion】 Fivefold dose of Banqing granules (500 mg/kg BW) was safe for pigs.

Key words: Banqing granules; pig; blood routine index; blood biochemical index; safety

中图分类号:

S853.7

丁梦婕, 王苹苹, 胡龙飞, 张秋娜, 陈静, 刘佳, 刘若男, 郝智慧. 板青颗粒对靶动物猪的安全性试验[J]. 中国畜牧兽医, 2024, 51(9): 4164-4173.

DING Mengjie, WANG Pingping, HU Longfei, ZHANG Qiuna, CHEN Jing, LIU Jia, LIU Ruonan, HAO Zhihui. Safety Test of Banqing Granules on Target Animal Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 4164-4173.

参考文献

[1] 杨黎宇,李燕玲,范舒钰,等.芪蓝复方对妊娠母猪免疫增强作用研究[J].中国畜牧兽医,2024,51(1):64-73. YANG L Y,LI Y L,FAN S Y, et al.Study on immunoenhancing effect of Qilan compound on pregnant sows[J].China Animal Husbandry & Veterinary Medicine,2024,51(1):64-73.(in Chinese)
[2] 刘莲,郭志廷,张康,等.中药参姜止痢合剂主要成分含量检测标准研究[J].中国畜牧兽医,2023,50(8):3421-3429. LIU L,GUO Z T,ZHANG K, et al.Study on quality standard for the main components of traditional Chinese medicine of Shenjiang Zhili mixture[J].China Animal Husbandry & Veterinary Medicine,2023,50(8):3421-3429.(in Chinese)
[3] 张亚倩,王亚男,郭上朝,等.2株金黄色葡萄球菌耐药性分析及中药对其部分耐药基因表达的影响[J].中国畜牧兽医,2023,50(12):5204-5212. ZHANG Y Q,WANG Y N,GUO S C, et al.Drug resistance analysis of 2Staphylococcus aureus strains and the influence of traditional Chinese medicine on expression of some drug resistance genes[J].China Animal Husbandry & Veterinary Medicine,2023,50(12):5204-5212.(in Chinese)
[4] 范秋雨,武建文,李春晓,等.苦豆子抗炎机制的网络药理学分析及试验验证[J].中国畜牧兽医,2023,50(7):2951-2965. FAN Q Y,WU J W,LI C X, et al.Network pharmacology analysis of anti-inflammatory mechanism of Sophora alopecuroides and experimental validation[J].China Animal Husbandry & Veterinary Medicine,2023,50(7):2951-2965.(in Chinese)
[5] 陈天宝,王娅妮,刘宝玲,等.中药抗猪繁殖与呼吸综合征病毒的作用机制研究进展[J].中国畜牧兽医，2023,50(5):2114-2129. CHEN T B,WANG Y N,LIU B L,et al.Research progress on the anti-Porcine reproductive and respiratory syndrome virus effect and mechanism of traditional Chinese medicine[J].China Animal Husbandry & Veterinary Medicine,2023,50(5):2114-2129.(in Chinese)
[6] 唐兴刚,罗胜军,袁明贵,等.牡荆水提物对蓖麻油腹泻小鼠的治疗作用研究[J].中国畜牧兽医，2024,51(2):799-808. TANG X G,LUO S J,YUAN M G,et al.Study on therapeutic effect of aqueous extract of Vitex negundo L.var.cannabifolia (Sieb.et Zucc.) Hand.-Mazz,on castor oil induced diarrhea in mice[J].China Animal Husbandry & Veterinary Medicine,2024,51(2):799-808.(in Chinese)
[7] 蔡燕子,袁子文,张兴平,等.基于网络药理学探究党参-甘草提取物增强断奶仔猪免疫功能的作用机制[J].中国畜牧兽医,2023,50(10):4279-4291. CAI Y Z,YUAN Z W,ZHANG X P, et al.Study on mechanism of Codonopsis pilosula-Glyeyrrhiza extract in enhancing immune functions of weaned piglets based on pharmacology[J].China Animal Husbandry & Veterinary Medicine,2023,50(10):4279-4291.(in Chinese)
[8] 温伟,吕金良,高振伟,等.浅谈现代中兽药的发展前景[J].中兽医学杂志,2010,1:50-53. WEN W,LYU J L,GAO Z W,et al.Discussion on the development prospect of modern Chinese veterinary drugs[J].Chinese Journal of Veterinary Science,2010,1:50-53.(in Chinese)
[9] 崔东安,王胜义,王磊,等.中兽药临床疗效评价研究及其关键科学问题[J].中国农业大学学报,2017,22(3):116-121. CUI D A,WANG S Y,WANG L,et al.Key research priorities for clinical efficacy evaluation oveterinary herbal medicines[J]. Journal of China Agricultural University,2017,22(3):116-121.(in Chinese)
[10] 中国兽药典委员会.中华人民共和国兽药典2020年版2部[M].北京：中国农业出版社,2020. CHINESE VETERINARY PHARMACOPOEIA COMMITTEE.Pharmacopoeia of the People’s Republic of China.2020 ed.Part 2[M].Beijing:China Agriculture Press,2020.(in Chinese)
[11] 农业部兽药评审中心.兽药研究技术指导原则汇编2006-2011年[M].北京：化学工业出版社,2012. MINISTRY OF AGRICULTURE VETERINARY DRUG EVALUATION CENTER.Compilation of Technical Guidelines for Veterinary Drug Research 2006-2011[M].Beijing:Chemical Industry Press,2012.(in Chinese)
[12] 肖珊珊,金郁,孙毓庆.板蓝根化学成分、药理及质量控制研究进展[J].沈阳药科大学学报,2003,6:455-459. XIAO S S,JIN Y,SUN Y Q.Recent progress in the studies of chemical constituents,pharmacological effects and quality control methods on the roots of Isatis indigotica[J].Journal of Shenyang Pharmaceutical University,2003,6:455-459.(in Chinese)
[13] 刘富康,方士元,曲映红.大青叶粗黄酮提取及其抑菌性研究[J].山东化工,2018,47(21):34-35. LIU F K,FANG S Y,QU Y H.Study on extraction and antibacterial activity of crude flavonoids from Folium isatidis[J].Shandong Chemical Industry,2018,47(21):34-35.(in Chinese)
[14] 闫延华.复方板青可溶性粉对IB的防治作用研究[D].郑州：河南农业大学,2017. YAN Y H.Green soluble power compound board role in prevention and cure of infectious bronchitis research[D].Zhengzhou：Henan Agricultural University,2017.(in Chinese)
[15] 黄继全.大青叶抗内毒素的实验研究[J].江西中医学院学报，2007,2:70-71. HUANG J Q.Experimental study on resistance to endotoxin in leaves of Chinese herb[J].Journal of Jiangxi University of Traditional Chinese Medicine,2007,2:70-71.(in Chinese)
[16] 闫峻,顾娟,冯硕,等.大青叶化学成分及抗氧化活性研究[J].质谱学报,2019,40(4):381-390. YAN J,GU J,FENG S,et al.Study of chemical constituents and antioxidant activity of Isatidis folium[J].Journal of Chinese Mass Spectrometry Society,2019,40(4):381-390.(in Chinese)
[17] 徐丽凤.中药在猪病防治中的应用[J].中国动物保健,2022,24(10):27-28. XU L F.Application of Chinese medicine in prevention and treatment of pig diseases[J]. China Animal Health,2022,24(10):27-28.(in Chinese)
[18] 张玉芳.中草药制剂在猪病防治中的应用[J].今日畜牧兽医,2023,39(9):71-73. ZHANG Y F.Application of Chinese herbal preparations in prevention and treatment of pig diseases[J].Today Animal Husbandry and Veterinary Medicine,2023,39(9):71-73.(in Chinese)
[19] 张月,吕宁,张世朋,等.试分析猪病防治中生物兽药与中兽药制剂的联合使用[J].吉林畜牧兽医,2023,44(8):17-18. ZHANG Y,LYU N,ZHANG S P,et al.Analysis on the combined use of biological and Chinese veterinary drugs in the prevention and treatment of pig diseases[J].Jilin Animal Husbandry and Veterinary Medicine.2023,44(8):17-18.(in Chinese)
[20] 邱月,阮景欣,张向荣,等.国内兽药新剂型及生产技术应用现状[J].中国饲料,2022,23:24-31. QIU Y,RUAN J X,ZHANG X R,et al.Application status of new dosage forms and production technology of veterinary drugs[J].China Feed,2022,23:24-31.(in Chinese)
[21] 张晋青,李梦怡,杨桂梅,等.板蓝根饲料添加剂对猪育肥性能与抗病力的影响[J].青海畜牧兽医杂志,2021,51(1):28-31. ZHANG J Q,LI M Y,YANG G M,et al.Effect of Radix isatidis feed additives on fattening performance and disease resistance in pig[J].Chinese Qinghai Journal of Animal and Veterinary Sciences,2021,51(1):28-31.(in Chinese)
[22] 唐才果,翁吉梅,苏厅,等.板蓝根对仔猪的生长及免疫抗病性能影响研究[J].猪业科学,2016,33(3):108-110. TANG C G,WENG J M,SU T,et al.Effect of isatis root on growth and immune resistance of piglets[J]. Swine Industry Science,2016,33(3):108-110.(in Chinese)
[23] 陈少康,王晓凤,李爽,等.猪血常规指标辅助评价健康种猪的初步研究[J].吉林畜牧兽医,2015,36(3):20-22. CHEN S K,WANG X F,LI S,et al.A preliminary study on the auxiliary evaluation of healthy breeding pigs by pig blood routine indexes[J].Jilin Animal Husbandry and Veterinary Medicine,2015,36(3):20-22.(in Chinese)
[24] 陈焕春，文心田，董常生.兽医手册[M].北京：中国农业出版社,2013. CHEN H C,WEN X T,DONG C S.Veterinary Manual[M].Beijing:China Agriculture Press,2013.(in Chinese)
[25] 宋欣,曹随忠,张龙超,等.猪红细胞生成素受体基因突变与血常规性状关联分析[J].畜牧兽医学报,2012,43(11):1697-1702. SONG X,CAO S Z,ZHANG L C,et al.Association of SNPs in erythropoietin receptor gene with blood parameters[J].Acta Veterinaria et Zootechnica Sinica,2012,43(11):1697-1702.(in Chinese)
[26] 王海,邓菲,耿雅丽.加米霉素注射液对靶动物猪的安全性试验研究[J].中国抗生素杂志,2018,43(12):1538-1542. WANG H,DENG F,GENG Y L.Study on the target animal safety of gamithromycin injection on pigs[J].Chinese Journal of Antibiotics,2018,43(12):1538-1542.(in Chinese)
[27] 敖妙,李晓玉,何颖,等.蒲地蓝消炎颗粒对靶动物猪的安全性试验[J].黑龙江畜牧兽医，2019,21:126-130. AO M,LI X Y,HE Y,et al.Safety test of Pudilan Xiaoyan granule on target animal pig[J].Heilongjiang Animal Science and Veterinary Medicine,2019,21:126-130.(in Chinese)
[28] 魏丽娟,刘欣,耿智霞,等.马波沙星注射液对靶动物猪的安全性研究[J].黑龙江畜牧兽医,2016,11:206-210. WEI L J,LIU X,GENG Z X,et al.Study on the safety of marbofloxacin injection in target animal pigs[J].Heilongjiang Animal Science and Veterinary Medicine,2016,11:206-210.(in Chinese)
[29] 张光磊,王勤华,范志勇,等.二丁酰环磷腺苷钙对肥育猪生长性能和血清生化指标的影响[J].动物营养学报,2017,29(1):264-270. ZHANG G L,WANG Q H,FAN Z Y,et al.Effects of dibutyryl cyclic adenosine monophosphate-calcium on growth performance and serum biochemical indices of finishing pigs[J].Chinese Journal of Animal Nutrition,2017,29(1):264-270.(in Chinese)
[30] INOUE T,YOSHID K,SASAKI E,et al.Effect of lycopene intake on the fasting blood glucose level:A systematic review with Meta-analysis[J].Nutrients,2022,15(1)：122.
[31] 周亚军.中草药饲料添加剂对生长猪生产性能及免疫功能影响的研究[D].长沙：湖南农业大学,2008. ZHOU Y J.The effects of Chinese herb feed additives on growing characteristics and immunity function of swine[D].Changsha:Hunan Agricultural University,2008.(in Chinese)
[32] 杨述林,任红艳,王恒,等.3个中国实验用小型猪品种血液生化指标分析[J].中国畜牧兽医，2007,34(4):75-78. YANG S L,REN H Y,WANG H,et al.Investigation of the blood biochemistry parameters of three Chinese indigenous laboratory miniature pig breeds[J].China Animal Husbandry & Veterinary Medicine, 2007,34(4):75-78.(in Chinese)
[33] 王巍,桑锐,葛冰洁,等.中药复方呼宁散安全性毒理学评价[J].中国畜牧兽医,2023,50(12):5222-5231. WANG W,SANG R,GE B J,et al.Toxicological safety evaluation of traditional Chinese medicine compound Huning powder[J].China Animal Husbandry & Veterinary Medicine,2023,50(12):5222-5231.(in Chinese)