复合菌培养物对蒙寒杂交肉羊生长性能、免疫功能及疫苗抗体效价的影响

doi:10.16431/j.cnki.1671-7236.2025.02.022

摘要/Abstract

摘要： 【目的】研究复合菌培养物对蒙寒杂交肉羊生长性能、免疫功能及疫苗抗体效价的影响。【方法】选取健康状况良好，体重相近的1.5月龄蒙寒杂交公羔羊40只，随机分为2组：试验组和对照组；每组4个重复，每个重复5只羔羊。对照组肉羊饲喂基础饲粮，试验组肉羊在饲喂基础饲粮的基础上每只羊额外饲喂复合菌培养物30 g/d，试验第16天给各组羊注射A型口蹄疫和羊痘疫苗，预试期7 d，正试期90 d。试验第0、30、60和90天晨饲前称重并采血分离血清，测定羔羊生长性能、免疫及抗氧化指标；试验第15、30和45天采血分离血清，测定A型口蹄疫和羊痘疫苗抗体效价。【结果】试验第90天,试验组肉羊平均体重显著高于对照组(P＜0.05)；试验0～90 d，试验组肉羊平均日采食量(ADFI)与料重比(F/G)与对照组无显著差异(P＞0.05)，平均日增重(ADG)较对照组显著提高(P＜0.05)。试验第90天，与对照组相比，试验组肉羊血清中溶菌酶(LZM)活性、白细胞介素-1β(IL-1β)、转化生长因子-β(TGF-β)、肿瘤坏死因子-α(TNF-α)含量、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC)均显著提高(P＜0.05)，丙二醛(MDA)含量显著降低(P＜0.05)；试验第45天，与对照组相比，试验组肉羊痘疫苗抗体效价显著提高(P＜0.05)，A型口蹄疫疫苗抗体效果差异不显著(P＞0.05)。【结论】添加复合菌培养物能显著提高蒙寒杂交肉羊生长性能，改善免疫功能与抗氧化功能，并能提高疫苗抗体效价。

关键词: 复合菌培养物; 蒙寒杂交肉羊; 生长性能; 免疫功能; 抗氧化功能; 疫苗抗体效价

Abstract: 【Objective】 The experiment was aimed to investigate the effects of compound bacteria culture on growth performance,immune function and vaccine antibody titer of Mongolian-Han crossbred mutton sheep. 【Method】 Forty 1.5-month-old Mongolian-Han crossbred male lambs with good health and similar body weight were randomly divided into 2 groups,control group and experimental group,with 4 replicates in each group and 5 lambs in each replicate.The lambs in control group was fed with basal diet,and the lambs in experimental group was fed with additional 30 g/d of compound bacterial culture per sheep on the basis of the basal diet.On the 16th day of the experiment,each lamb was injected with type A foot-and-mouth disease and sheeppox vaccines.The pre-trial period was 7 days and the formal period was 90 days.On the 0th,30th,60th and 90th day of the experiment,the lambs were weighed,blood was collected and serum was separated before morning feeding,and the growth performance,immune and antioxidant indexes were measured.On the 15th,30th and 45th day of the experiment,blood was collected and serum was separated to determine the antibody titers of type A foot-and-mouth disease and sheep pox vaccine. 【Result】 On the 90th day of the experiment,compared with control group,the average body weight of mutton sheep in experimental group were significantly increased (P＜0.05).From 0 to 90 days of the experiment,there was no significant difference in the average daily feed intake (ADFI) and feed to gain ratio (F/G) of mutton sheep between experimental group and control group (P＞0.05),but the average daily gain (ADG) of mutton sheep in experimental group was significantly increased compared to control group (P＜0.05).On the 90th day of the experiment,compared with the control group,the activity of lysozyme (LZM),contents of interleukin-1β (IL-1β),transforming growth factor-β (TGF-β),tumor necrosis factor-α (TNF-α),activities of catalase (CAT) and glutathione peroxidase (GSH-Px),and total antioxidant capacity (T-AOC) in serum of mutton sheep in experimental group were significantly increased (P＜0.05),while the content of malondialdehyde (MDA) was significantly decreased (P＜0.05).On the 45th day of the experiment,compared with control group,the antibody titer of the sheeppox vaccine of mutton sheep in experimental group was significantly increased (P<0.05),while the antibody titer of the foot-and-mouth disease type A vaccine was not significantly different (P＞0.05). 【Conclusion】 The addition of compound bacteria culture could significantly improve the growth performance,immune function and antioxidant function of Mongolian-Han crossbred mutton sheep,and increase the titer of vaccine antibody.

Key words: compound bacteria culture (CBC); Mongolian-Han crossbred mutton sheep; growth performance; immune function; antioxidant function; vaccine antibody titer

中图分类号:

S816.7

刘世雄, 李啸, 李薛强, 徐子萱, 李松建, 杜蕊, 薛晨, 刘大程. 复合菌培养物对蒙寒杂交肉羊生长性能、免疫功能及疫苗抗体效价的影响[J]. 中国畜牧兽医, 2025, 52(2): 718-729.

LIU Shixiong, LI Xiao, LI Xueqiang, XU Zixuan, LI Songjian, DU Rui, XUE Chen, LIU Dacheng. Effects of Compound Bacteria Culture on Growth Performance,Immune Function and Vaccine Antibody Titer of Mongolian-Han Crossbred Mutton Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 718-729.

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