姜黄素对热应激条件下延边黄牛育成牛生长性能、饲料养分表观消化率和血液指标的影响

doi:10.16431/j.cnki.1671-7236.2025.02.016

摘要/Abstract

摘要： 【目的】本研究旨在探讨姜黄素(curcumin,CUR)对热应激条件下育成牛生长性能、营养物质表观消化率和血清生化指标的影响，探究热应激条件下肉牛饲粮中适宜的姜黄素添加水平。【方法】选取健康、体重接近(286.36 kg±3.95 kg)的32头延边黄牛育成牛，随机分成4组，每组8个重复，每个重复1头牛。各组姜黄素添加水平分别为0、100、200、300 mg/kg DM饲粮，分别为对照组、100 CUR组、200 CUR组、300 CUR组。预饲期7 d，正式试验期30 d。试验期间测定每日各时间点牛舍温度及相对湿度，计算温湿指数(temperature humidity index,THI)(试验期间牛舍THI≥72，试验牛均处于热应激状态)；试验第31天早上，试验牛称重、测量体尺指标；颈静脉采血，检测血清生化指标；采用全收粪法收集新鲜粪便，测定饲料养分表观消化率。【结果】与对照组相比，200 CUR组牛平均日增重、体斜长和胸围均显著提高(P＜0.05)，料重比显著降低(P＜0.05)；饲料粗脂肪、中性洗涤纤维和酸性洗涤纤维的表观消化率均显著提高(P＜0.05)；血清白蛋白、甲状腺素含量显著提高(P＜0.05)，谷草转氨酶、谷丙转氨酶活性、甘油三酯含量显著降低(P＜0.05)；丙二醛水平显著降低(P＜0.05)。与对照组相比，各试验组牛体高、粗蛋白质消化率、血清总蛋白、球蛋白含量均显著提高(P＜0.05)；血清热休克蛋白70、皮质醇水平均显著降低(P＜0.05)；100 CUR组牛血清免疫球蛋白A显著提高(P＜0.05)；200 CUR和300 CUR组牛血清超氧化物歧化酶、谷胱甘肽-过氧化物酶活性及总抗氧能力均显著提高(P＜0.05)。【结论】在热应激条件下，饲粮中添加200 mg/kg DM 姜黄素可以提高育成期延边黄牛的生长性能和饲料养分表观消化率，提高机体抗氧化能力。

关键词: 姜黄素; 延边黄牛; 血液指标; 生长性能; 热应激

Abstract: 【Objective】 This study aimed to investigate the effects of curcumin (CUR) on growth performance,apparent digestibility of nutrients and serum biochemical indexes of breeding cattle under heat stress condition,and to explore the appropriate curcumin supplementation level in beef cattle diets under heat stress condition. 【Method】 Thirty-two healthy Yanbian Yellow breeding cattle,weighing 286.36 kg±3.95 kg,were randomly divided into four groups,with eight replicates in each group and one cow in each replicate.The curcumin supplemental levels for each group were set at 0,100,200 and 300 mg/kg DM diet respectively，named as control group,100 CUR,200 CUR,and 300 CUR groups.The prefeeding period was 7 days and the test period was 30 days.During the experiment,the temperature and relative humidity of the cowshed were measured at different time points every day,and the temperature and humidity index (THI) was calculated.During experimental period, THI≥72,the experimental cattle were considered to be in a state of heat stress.On the morning of the 31st day,the experimental cattle were weighed and their body size was assessed.Blood was drawn from the jugular vein to test serum biochemical indicators.The total fecal collection method was used to collect fresh feces for the measurement of apparent digestibility of feed nutrients. 【Result】 Compared to control group,cattle in 200 CUR group exhibited significant improvements in average daily weight gain,body depth,and chest circumference (P＜0.05),alongside a notable reduction in feed-to-gain ratio (P＜0.05).The apparent digestibility of crude fat,neutral detergent fiber and acid detergent fiber were significantly enhanced (P＜0.05),serum albumin and thyroid hormone levels were markedly elevated (P＜0.05),whereas activities of glutamate-oxaloacetic transaminase and glutamate-pyruvic transaminase as well as triglyceride content were significantly diminished (P＜0.05),furthermore,malondialdehyde levels showed a significant decrease (P＜0.05) in 200 CUR group.Compared to control group,the body height,crude protein digestibility,serum total protein and globulin contents were significantly increased (P＜0.05),the levels of heat shock protein 70 and cortisol were significantly decreased (P＜0.05) in three experimental groups.Serum immunoglobulin A was significantly increased in 100 CUR group (P＜0.05).The activities of superoxide dismutase,glutathione peroxidase,and total antioxidant capacity in bovine serum were significantly elevated in 200 CUR and 300 CUR groups (P＜0.05). 【Conclusion】 Adding 200 mg/kg DM curcumin to the diet of Yanbian Yellow breeding cattle during heat stress could improve their growth performance,apparent digestibility of feed nutrients,and antioxidant capacity.

Key words: curcumin; Yanbian Yellow cattle; blood index; growth performance; heat stress

中图分类号:

S816.7

钟明, 耿龙君, 崔莲花, 徐紫嫣, 严昌国, 冯健, 蔡洪范, 张旭. 姜黄素对热应激条件下延边黄牛育成牛生长性能、饲料养分表观消化率和血液指标的影响[J]. 中国畜牧兽医, 2025, 52(2): 655-667.

ZHONG Ming, GENG Longjun, CUI Lianhua, XU Ziyan, YAN Changguo, FENG Jian, CAI Hongfan, ZHANG Xu. Effects of Curcumin on Growth Performance, Apparent Digestibility of Feed Nutrients and Blood Indexes of Yanbian Yellow Breeding Cattle Under Heat Stress[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 655-667.

参考文献

[1] 陈豪,兰剑,聂春桃,等.葛根素和橙皮苷对热应激条件下锦江黄牛养分表观消化率和粪便菌群组成的影响[J].动物营养学报,2023,35(1):360-368.CHEN H,LAN J,NIE C T,et al.Effects of puerarin and hesperidin on nutrient apparent digestibility and fecal microflora of Jinjiang Yellow cattle under heat stress[J].Chinese Journal of Animal Nutrition,2023,35(1):360-368.(in Chinese)
[2] BECKFORD R C,ELLESTAD L E,PROSZKOWIEC-WEGLARZ M,et al.Effects of heat stress on performance,blood chemistry,and hypothalamic and pituitary mRNA expression in broiler chickens[J].Poultry Science,2020,99(12):6317-6325.
[3] SEJIAN V,BHATTA R,GAUGHAN J B,et al.Adaptation of animals to heat stress[J].Animal,2018,12(s2):s431-s444.
[4] DE OLIVERIRA M J K,VALK M,MELO A D B,et al.Feeding behavior of finishing pigs under diurnal cyclic heat stress[J].Animals,2023,13(5):908.
[5] RIUS A G,KAUFMAN J D,LI M M,et al.Physiological responses of Holstein calves to heat stress and dietary supplementation with a postbiotic from Aspergillus oryzae[J].Scientific Reports,2022,12(1):1587.
[6] AFSAL A,SEJIAN V,BAGATH M,et al.Heat stress and livestock adaptation:Neuro-endocrine regulation[J].International Journal of Veterinary and Animal Medicine,2018,1(2)：1-8.
[7] HABASHY W S,MILFORT M C,REKAYA R,et al.Cellular antioxidant enzyme activity and biomarkers for oxidative stress are affected by heat stress[J].International Journal of Biometeorology,2019,63:1569-1584.
[8] BEJIAOUI B,SDIRI C,BEN SOUF I,et al.Physicochemical properties,antioxidant markers,and meat quality as affected by heat stress:A review[J].Molecules,2023,28(8):3332.
[9] 郑洁怡,杨舒黎,赵开玲,等.热应激对反刍动物瘤胃功能及潜在耐热标志物的影响[J].中国畜牧兽医,2024,51(3):1041-1049.ZHENG J Y,YANG S L,ZHAO K L,et al.Effects of heat stress on rumen function and potential heattolerance markers in ruminants[J].China Animal Husbandry & Veterinary Medicine,2024,51(3):1041-1049.(in Chinese)
[10] 刘佩.复方中草药添加剂缓解热应激对低产奶牛生产性能的影响[D].南昌：江西农业大学,2023.LIU P.Effects of compound Chinese herbal additiveson relieving heat stress on performance of low-yielding dairy cows[D].Nanchang:Jiangxi Agricultural University,2023.(in Chinese)
[11] GAYATHRI K,BHASKARAN M,SELVAM C,et al.Nano formulation approaches for curcumin delivery—A review[J].Journal of Drug Delivery Science and Technology,2023,82:104326.
[12] 陈煜淳,林勇,刘勇,等.姜黄素的应用、制备及改性研究进展[J].现代食品科技,2024,40(6):327-335.CHEN Y C,LIN Y,LIU Y,et al.Research progress application,on preparation and modification of curcumin[J].Modern Food Science and Technology,2024,40(6):327-335.(in Chinese)
[13] 付江文,张海波,欧阳红兵,等.姜黄素的抗氧化功能及其在畜禽养殖中的应用研究进展[J].中国畜牧杂志,2022,58(10):14-18.FU J W,ZHANG H B,OUYANG H B,et al.Advances in the antioxidant function and application of curcumin in animal production[J].Chinese Journal of Animal Science,2022,58(10):14-18.(in Chinese)
[14] 陈鑫宇,潘士锋,邢华,等.姜黄素与肠道微生物间相互作用研究进展[J].动物营养学报,2023,35(2):782-788.CHEN X Y,PAN S F,XING H,et al.Research progress on interaction between curcumin and gut microbiota[J].Chinese Journal of Animal Nutrition,2023,35(2):782-788.(in Chinese)
[15] ARDEBILI A,POURIAYEVALI M H,ALESHIKH S,et al.Antiviral therapeutic potential of curcumin:An update[J].Molecules,2021,26(22):6994.
[16] PENG Y,AO M,DONG B,et al.Anti-inflammatory effects of curcumin in the inflammatory diseases:Status,limitations and countermeasures[J].Drug Design,Development and Therapy,2021:4503-4525.
[17] 楼芳芳,陈雨诗,刘禹熙,等.姜黄素对育肥期金华猪生长性能、肉品质、脂质代谢及肠道微生物的影响[J].中国畜牧杂志,2023,59(8):308-315.LOU F F,CHEN Y S,LIU Y X,et al.Effects of curcumin on growth performance,meat quality,lipid metabolism and intestinal microorganisms of Jinhua pigs during fattening period[J].Chinese Journal of Animal Science,2023,59(8):308-315.(in Chinese)
[18] FU Y S,CHEN T H,WENG L,et al.Pharmacological properties and underlying mechanisms of curcumin and prospects in medicinal potential[J].Biomedicine & Pharmacotherapy,2021,141:111888.
[19] 严昌国,王勇,朴圣哲,等.延边黄牛牛肉品质特性的研究[J].黄牛杂志,2004,3:5-7.YAN C G,WANG Y,PIAO S Z,et al.Study on the beef quality traits of Yanbian cattle[J].Journal of Yellow Cattle Science,2004,3:5-7.(in Chinese)
[20] 岳子奇,姚小鹤,王之盛,等.去势和热应激对筠连黄牛生产性能、瘤胃发酵和血液生化指标的影响[J].中国农业大学学报,2024,29(1):127-139.YUE Z Q,YAO X H,WANG Z S,et al.Effects of castration and heat-stress on production performance,rumen fermentation and blood biochemical indexes of Junlian cattle[J].Journal of China Agricultural University,2024,29(1):127-139.(in Chinese)
[21] TUCKER C B,ROGERS A R,SCHUTZ K E.Effect of solar radiation on dairy cattle behaviour,use of shade and body temperature in a pasture-based system[J].Applied Animal Behaviour Science,2008,109(2-4):141-154.
[22] BERMAN A.Estimates of heat stress relief needs for Holstein dairy cows[J].Journal of Animal Science,2005,83(6):1377.
[23] 林成艳.大白猪重要经济性状全基因组关联分析及全基因组选择准确率的比较研究[D].成都：四川农业大学,2023.LIN C Y.Genome-wide association study of important economic traits and comparative study on the accuracy of genome selection in Large Yorkshire pigs[D].Chengdu:Sichuan Agricultural University,2023.(in Chinese)
[24] 张丽英.饲料分析及饲料质量检测技术[M].北京:中国农业大学出版社,2016.ZHANG L Y.Feed Analysis and Feed Quality Detection Technology[M].Beijing:China Agriculture University Press，2016.(in Chinese)
[25] 陈雨诗,刘禹熙,杨童寓丹,等.饲粮中添加姜黄素和二十二碳六烯酸对育肥猪生长性能、胴体性状、肉品质及血清生化、抗氧化和免疫指标的影响[J].动物营养学报,2024,36(3):1537-1548.CHEN Y S,LIU Y X,YANG T Y D,et al.Effects of dietary curcumin and docosahexaenoic acid on growth performance,carcass traits,meat quality and serum biochemical,antioxidant and immune indexes of finishing pigs[J].Chinese Journal of Animal Nutrition,2024,36(3):1537-1548.(in Chinese)
[26] 杨灿,张玉婷,唐小武,等.姜黄素对湘黄鸡生长性能、血清生化指标和抗氧化能力的影响[J].动物营养学报,2021,33(12):6749-6756.YANG C,ZHANG Y T,TANG X W,et al.Effects of curcumin on growth performance,serum biochemical indexes and antioxidant capacity of Xianghuang broilers[J].Chinese Journal of Animal Nutrition,2021,33(12):6749-6756.(in Chinese)
[27] NIU Y,HE J,ZHAO Y,et al.Effect of curcumin on growth performance,inflammation,insulin level,and lipid metabolism in weaned piglets with IUGR[J].Animals,2019,9(12):1098.
[28] 洪奇华,李鑫,徐求文,等.姜黄素对断奶仔猪肠道屏障、抗氧化和线粒体功能的影响[J].中国畜牧杂志,2022,58(6):251-255.HONG Q H,LI X,XU Q W,et al.Effects of curcumin on intestinal barrier,oxidative stress and mitochondrial function in weaned piglets[J].Chinese Journal of Animal Science,2022,58(6):251-255.(in Chinese)
[29] 金远铭.不同处理的人参副产物对延边黄牛生产性能的影响[D].延吉：延边大学,2019.JIN Y M.Effects of ginseng by-products of differentprocessed on production performance of Yanbian Yellow cattle[D].Yanji:Yanbian University,2019.(in Chinese)
[30] 王梓蓓,戴思凡,赵树生,等.邓川牛体重与体尺指标的主成分分析[J].中国畜牧杂志,2024,60(7):106-113.WANG Z B,DAI S F,ZHAO S S,et al.Principal component analysis of body weight and body size of Dengchuan cattle[J].Chinese Journal of Animal Science,2024,60(7):106-113.(in Chinese)
[31] 和立文.全株玉米青贮品质评价及其对肉牛育肥性能和牛肉品质的影响[D].北京：中国农业大学,2017.HE L W.Quality evaluation of corn silage and its effect on the growth performance and beef quality of finishing cattle[D].Beijing:China Agricultural University,2017.(in Chinese)
[32] 田光元,郝小燕,任国栋,等.饲粮中添加姜黄素对舍饲羔羊生长性能、营养物质表观消化率、能氮代谢和血清生化指标的影响[J].动物营养学报,2022,34(9):5964-5973.TIAN G Y,HAO X Y,REN G D,et al.Effects of dietary curcumin on growth performance,nutrient apparent digestibility,energy and nitrogen metabolism and serum biochemical indices of barn feeding lambs[J].Chinese Journal of Animal Nutrition,2022,34(9):5964-5973.(in Chinese)
[33] 欧阳坤,魏立民,孙瑞萍,等.姜黄素对海南屯昌猪生长性能、饲料养分表观消化率和血清生化指标的影响[J].中国畜牧兽医,2023,50(5):1867-1875.OUYANG K,WEI L M,SUN R P,et al.Effects of curcumin on growth performance,feed apparent digestibility and serum biochemical indexes in Hainan Tunchang pigs[J].China Animal Husbandry & Veterinary Medicine,2023,50(5):1867-1875.(in Chinese)
[34] GLOMBOWSKY P,VOLPATO A,CAMPIGOTTO G,et al.Dietary addition of curcumin favors weight gain and has antioxidant,anti-inflammatory and anticoccidial action in dairy calves[J].Revista Colombiana de Ciencias Pecuarias,2020,33(1):16-31.
[35] LI S,WU F,ZHAO M,et al.Effects of curcumin on the growth performance,apparent nutrient digestibility,intestinal morphology,digestive enzyme activity,and antioxidant capacity of meat rabbits[J].Italian Journal of Animal Science,2023,22(1):222-229.
[36] ZHAO S,MIN L,ZHENG N,et al.Effect of heat stress on bacterial composition and metabolism in the rumen of lactating dairy cows[J].Animals,2019,9(11):925.
[37] LI Q S,WANG R,MA Z Y,et al.Dietary selection of metabolically distinct microorganisms drives hydrogen metabolism in ruminants[J].The ISME Journal,2022,16(11):2535-2546.
[38] TIAN G,ZHANG X,HAO X,et al.Effects of curcumin on growth performance,ruminal fermentation,rumen microbial protein synthesis,and serum antioxidant capacity in housed growing lambs[J].Animals,2023,13(9):1439.
[39] ZHENG D,HUANG C,HUANG H,et al.Antibacterial mechanism of curcumin:A review[J].Chemistry & Biodiversity,2020,17(8):e2000171.
[40] 蒲启建,王之盛,彭全辉,等.热应激对不同品种(系)青年肉牛生产性能、营养物质表观消化率及血液生化指标的影响[J].动物营养学报,2017,29(9):3120-3131.PU Q J,WANG Z S,PENG Q H,et al.Effects of heat stress on performance,nutrient apparent digestibility and blood biochemical indices of different breeds of young beef cattle[J].Chinese Journal of Animal Nutrition,2017,29(9):3120-3131.(in Chinese)
[41] 钟港,陈坤,林文超,等.不同饲粮能量水平对湘南黄牛生长性能、血液生化指标及抗氧化力的影响[J].中国畜牧杂志,2021,57(8):219-223.ZHONG G,CHEN K,LIN W C,et al.Effects of different dietary energy levels on growth performance,blood biochemical indexes and antioxidant capacity of Xiangnan Yellow cattle[J].Chinese Journal of Animal Science,2021,57(8):219-223.(in Chinese)
[42] 杨梓曼,尚相龙,陈豪,等.热应激对肉牛血清生化指标、瘤胃发酵参数及微生物区系的影响[J].动物营养学报,2022,34(7):4487-4497.YANG Z M,SHANG X L,CHEN H,et al.Effects of heat stress on serum biochemical indexes,rumen fermentation parameters and microflora of beef cattle[J].Chinese Journal of Animal Nutrition,2022,34(7):4487-4497.(in Chinese)
[43] 宋珍全.利用转录组筛选鸡内脏器官中急性热应激相关的关键基因[D].武汉：华中农业大学,2023.SONG Z Q.Screenimg for key genes related to acuteheat stress in chicken visceral organs using transcriptomes[D].Wuhan:Huazhong Agricultural University,2023.(in Chinese)
[44] 郑宇慧,欧阳潼,薛圣霖,等.热应激对奶牛乳腺功能的影响及机制研究进展[J].动物营养学报,2021,33(11):6024-6036.ZHENG Y H,OUYANG T,XUE S L,et al.Research progress on effects and mechanism of heat stress on mammary gland function of dairy cows[J].Chinese Journal of Animal Nutrition,2021,33(11):6024-6036.(in Chinese)
[45] 余维,俞杭锋,管家齐.姜黄素缓解2型糖尿病小鼠肝脏的代谢紊乱[J].中华中医药学刊,2015,33(12):2895-2897.YU W,YU H F,GUAN J Q.Benefits of curcumin on hepatic metabolic disorders in type 2 diabetic mice[J].Chinese Archives of Traditional Chinese Medicine,2015,33(12):2895-2897.(in Chinese)
[46] 杜冬华.骆驼乳清蛋白对热应激致肝损伤的预防作用及机制研究[D].呼和浩特：内蒙古农业大学,2022.DU D H.Preventive effect and mechanism of camel whey protein on liver injury induced by heat stress[D].Hohhot:Inner Mongolia Agricultural University,2022.(in Chinese)
[47] 杨泰.鞣花酸对热应激肉鸡肠道损伤保护效应及机制研究[D].长沙：湖南农业大学,2022.YANG T.Study on the protective effect and mechanism of ellagic acid on intestinal damage in heat-stressed broiler chickens[D].Changsha:Hunan Agricultural University,2022.(in Chinese)
[48] 黄雪梅.转录组测序分析姜黄素抑制肾上腺皮质癌增殖和侵袭的作用机制[D].南宁：广西医科大学,2021.HUANG X M.Curcumin inhibits the growth and induces apoptosis of adrenocortical carcinoma:identification of potential candidate genes and pathways by transcriptome analysis[D].Nanning:Guangxi Medical University,2021.(in Chinese)
[49] 刘松松,项阳,王剑.热休克蛋白70在细胞凋亡途径中的作用研究进展[J].中国畜牧杂志,2020,56(8):45-49.LIU S S,XIANG Y,WANG J.Effect of heat shock protein 70 on the process of cellular apoptosis[J].Chinese Journal of Animal Science,2020,56(8):45-49.(in Chinese)
[50] 梅杰,吕美,曾佳,等.金银花提取物对热应激奶山羊生产性能、乳成分、血清生化指标和抗氧化能力的影响[J].动物营养学报,2022,34(9):5995-6002.MEI J,LYU M,ZENG J,et al.Effects of honeysuckle extract on performance,milk composition,serum biochemical indexes and antioxidant capacity of heat-stressed dairy goats[J].Chinese Journal of Animal Nutrition,2022,34(9):5995-6002.(in Chinese)
[51] 雷小文,刘珍妮,孔智伟,等.蚯蚓液对高温环境下番鸭生长性能、血清热应激指标、激素水平及肠道功能的影响[J].中国畜牧兽医,2023,50(12):4826-4836.LEI X W,LIU Z N,KONG Z W,et al.Effects of earthworm hydrolysate on growth performance,heat stress index,hormone levels in serum and intestinal function of muscovy ducks under high temperature environment[J].China Animal Husbandry & Veterinary Medicine,2023,50(12):4826-4836.(in Chinese)