香芹酚对水貂生长性能、血清生化指标及肠道菌群的影响

doi:10.16431/j.cnki.1671-7236.2024.08.006

摘要/Abstract

摘要： 【目的】探究饲粮中添加不同浓度香芹酚对育成期水貂生长性能、血清生化指标及肠道菌群的影响,从而筛选出对水貂各方面有益的香芹酚最适浓度。【方法】选取40只短毛黑公貂,随机分为4组,每组10只,每只为1个重复,对照组水貂饲喂基础饲粮,A、B、C组水貂分别在基础饲粮基础上添加100、200 和300 mg/kg香芹酚。预试期7 d,正试期42 d。饲养期结束后进行生长性能、血清生化指标和肠道菌群检测。【结果】饲粮中添加香芹酚对水貂生长性能(除终末体重外)无显著影响(P＞0.05);300 mg/kg香芹酚可显著提高水貂过氧化氢酶(CAT)活性及隐窝深度(P＜0.05);100 mg/kg香芹酚可显著提高水貂免疫球蛋白A(IgA)含量(P＜0.05);200和300 mg/kg香芹酚可极显著降低水貂丙二醛(MDA)含量(P＜0.01),显著提高水貂空肠绒毛高度(P＜0.05)。高通量测序结果显示,丰度＞1%的菌群共检测出以厚壁菌门(Firmicutes)为主的9类菌门,以及以乳球菌属(Lactococcus)、链球菌(Streptococcus)等为主的43个菌属。【结论】在水貂饲粮中添加量为300 mg/kg香芹酚可改善空肠肠道发育及肠道菌群结构,提升水貂的健康状况。

关键词: 香芹酚; 水貂; 生长性能; 血清生化指标; 肠道菌群

Abstract: 【Objective】 The aim of this study was to explore the effects of adding different concentrations of carvacrol to the diet on the growth performance,serum biochemical indexes and intestinal microbiota of mink during the breeding period,so as to find out the optimal concentration of carvacrol which was beneficial to mink in various aspects.【Method】 Forty Short-haired black mink were randomly divided into four groups of 10 minks each,with one replication for each mink.Minks in control group were fed with basal diet,while minks in groups A,B and C were supplemented with carvacrol at concentrations of 100,200 and 300 mg/kg in addition to the basic diet,respectively.The pre-test period was 7 d and the main test period was 42 d.The growth performance,serum biochemical indexes and intestinal microbiota were tested at the end of the feeding period.【Result】 The results showed that adding carvacrol had no effect on the growth performance (except for final body weight) of minks (P＞0.05).300 mg/kg carvacrol could significantly increase the activity of catalase (CAT) and crypt depth in minks (P＜0.05).100 mg/kg carvacrol could significantly increase the content of immunoglobulin A (IgA) in minks (P＜0.05).200 and 300 mg/kg carvacrol could extremely significantly reduce the content of malondialdehyde (MDA) (P＜0.01),and significantly increase the jejunal villus heigth in minks (P＜0.05).High throughput sequencing results showed that a total of 9 phyla dominated by Firmicutes were detected in the bacterial community with abundance greater than 1%,and 43 genera dominated by Lactococcus and Streptococcus.【Conclusion】 The addition of 300 mg/kg carvacrol to mink feed could improve jejunal intestinal development and intestinal microbiota structure,and enhance the health status of minks.

Key words: carvacrol; minks; growth performance; serum biochemical indexes; intestinal microbiota

中图分类号:

S864.5

石华丽, 陈嗣麒, 云祥鸿, 杨懿纯, 张爱武. 香芹酚对水貂生长性能、血清生化指标及肠道菌群的影响[J]. 中国畜牧兽医, 2024, 51(8): 3279-3287.

SHI Huali, CHEN Siqi, YUN Xianghong, YANG Yichun, ZHANG Aiwu. Effect of Carvacrol on Growth Performance,Serum Biochemical Indexes and Intestinal Microbiota of Minks[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(8): 3279-3287.

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