双氢青蒿素对新孢子虫感染雄性小鼠精子质量和生精功能基因的影响

doi:10.16431/j.cnki.1671-7236.2025.04.031

摘要/Abstract

摘要： 【目的】新孢子虫病是引起孕畜流产、死胎或新生儿运动障碍等临床症状的一种原虫病，目前尚无有效的药物防治该病。试验旨在确定双氢青蒿素对新孢子虫感染雄性小鼠精子质量和生精功能基因的影响作用，以期达到抗虫效果。【方法】以新孢子虫感染BALB/c雄性小鼠模型为研究对象，进行双氢青蒿素灌胃给药，给药剂量为0.2 g/kg，连续给药7 d，同时设置空白对照组和模型组。给药后检测雄性小鼠的生殖器官指数和精子质量，采用改良巴氏染色法观察精子形态变化。在给药第7、14、21、35、42天分别检测生精功能基因C-kit、Plzf、Sycp3、Stra8的表达情况。在给药第7天，应用流式细胞术检测生精细胞的凋亡情况。【结果】试验成功建立了新孢子虫感染雄性小鼠模型，与空白对照组相比，模型组小鼠生殖器官指数、精子密度、精子活力均极显著降低(P＜0.01)，精子畸形率极显著升高(P＜0.01)，C-kit、Plzf、Sycp3、Stra8基因表达量显著或极显著降低(P＜0.05；P＜0.01)，生精细胞凋亡率极显著升高(P＜0.01)；双氢青蒿素组小鼠生殖器官指数(除第21天外)、精子密度、精子活力均极显著或显著降低(P＜0.01；P＜0.05)，精子畸形率极显著升高(P＜0.01)，Plzf、C-kit、Sycp3和Stra8基因表达量均降低，部分时间点达显著水平(P＜0.05)，生精细胞凋亡率极显著升高(P＜0.01)。与模型组相比，双氢青蒿素组小鼠生殖器官指数显著或极显著升高(P＜0.05；P＜0.01)，精子密度增加，精子活力增强，精子畸形率极显著降低(P＜0.01)，C-kit、Plzf、Sycp3和Stra8基因表达量均升高，部分时间点达显著或极显著水平(P＜0.05；P＜0.01)，生精细胞凋亡率极显著降低(P＜0.01)。【结论】双氢青蒿素能够调节雄性小鼠生殖器官指数，提高精子质量，减少畸形精子的产生，提高生精功能基因Sycp3、C-kit、Plzf、Stra8的表达量，改善新孢子虫对雄性小鼠精子质量和生精功能基因的损伤。本研究结果为抗新孢子虫有效药物的研究提供了参考依据。

关键词: 新孢子虫; 双氢青蒿素; 生殖器官指数; 精子质量; 生精功能基因

Abstract: 【Objective】 Neosporidiosis is a protozoan disease that causes clinical symptoms such as abortion,stillbirth or movement disorder in pregnant animals.The aim of this experiment was to determine the effect of dihydroartemisinin on sperm quality and spermatogenic function genes of male mice infected with Neosporidium neosporidium, in order to acheive anti-Neosporidium neosporidium effect.【Method】 BALB/c male mice model infected with Neosporidium neosporidium were taken as the study object,and dihydroartemisinin was administered intragastically at a dose of 0.2 g/kg for 7 days.Meanwhile,blank control and model groups were set up.After administration,the reproductive organ index and sperm quality of male mice were measured,and the morphological changes of sperm were observed by modified Pap staining.The expression of spermatogenic function genes C-kit,Plzf,Sycp3 and Stra8 were detected at 7th,14th,21st,35th and 42nd days after administration.On the 7th day of administration,the apoptosis of spermatogenic cells was detected by flow cytometry.【Result】 The male mice model of Neosporidium neosporidium infection were established successfully.Compared with blank control group,the reproductive organ index,sperm density and sperm motility of mice in model group were extremely significantly decreased (P＜0.01),while the sperm malformation rate was extremely significantly increased (P＜0.01).The expressions of C-kit,Plzf,Sycp3 and Stra8 genes were significantly or extremely significantly decreased (P＜0.05 or P＜0.01).The apoptosis rate of spermatogenic cells was extremely significantly increased (P＜0.01).In dihydroartemisinin group,the reproductive organ index (excluding the 21st day),sperm density and sperm motility were significantly or extremely significantly decreased (P＜0.05 or P＜0.01),while the sperm malformation rate was significantly increased (P＜0.01).The expression of C-kit,Plzf,Sycp3 and Stra8 genes were decreased,reaching significant levels at some time points (P＜0.05).The apoptosis rate of spermatogenic cells was extremely significantly increased (P＜0.01).Compared with model group,the reproductive organ index of mice in dihydroartemisinin group was significantly or extremely significantly increased (P＜0.05 or P＜0.01),the sperm density and sperm motility were increased,the sperm malformation rate was significantly decreased (P＜0.01).The expression of C-kit,Plzf,Sycp3 and Stra8 genes were increased,reaching significant or extremely significant levels at some time points (P＜0.05 or P＜0.01),and the apoptosis rate of spermatogenic cells was extremely significantly decreased (P＜0.01).【Conclusion】 Dihydroartemisinin could regulate the reproductive organ index of male mice,improve sperm quality,reduce the production of malformed sperm,increase the expression of spermatogenic function genes Sycp3,C-kit,Plzf and Stra8,and improve the damage of Neosporidium neosporidium on spermatogenic sperm quality and spermatogenic function genes of male mice.The results provided a reference for the research of effective anti-Neosporidium neosporidium drugs.

Key words: Neosporidium neosporidium; dihydroartemisinin; reproductive organ index; sperm quality; spermatogenic function genes

中图分类号:

S852.72⁺3

赵芳琳, 刘萌, 唐泽宇, 赵健豪, 李璐, 贾立军. 双氢青蒿素对新孢子虫感染雄性小鼠精子质量和生精功能基因的影响[J]. 中国畜牧兽医, 2025, 52(4): 1796-1806.

ZHAO Fanglin, LIU Meng, TANG Zeyu, ZHAO Jianhao, LI Lu, JIA Lijun. Effects of Dihydroartemisinin on Sperm Quality and Spermatogenic Function Gene in Male Mice Infected with Neosporidium neosporidium[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1796-1806.

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