东北地区秋季饲喂赖氨酸锌对湖羊种公羊血浆生化指标与精子质量的影响

doi:10.16431/j.cnki.1671-7236.2024.02.013

摘要/Abstract

摘要： 【目的】试验旨在研究东北地区秋季饲喂赖氨酸锌对湖羊血浆生化指标与精子质量的影响。【方法】选择体况良好的1~2岁湖羊种公羊32只随机平均分为4组,分别补饲0、25、50、100 mg/d赖氨酸锌。试验于2022年8月至2022年10月在黑龙江伊春开展,平均气温10.3 ℃。试验预试期7 d,正试期56 d。分别于试验第28、42及56天采集湖羊颈静脉血,测定血浆睾酮、褪黑素、白细胞介素-2、白细胞介素-4、白细胞介素-6、白细胞介素-10、促肾上腺皮质激素和肿瘤坏死因子-α水平,以及血浆总抗氧化能力、过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性及丙二醛水平。采集湖羊精液测定精子活率、质膜完整率及畸形率。【结果】第28天时50 mg/d组湖羊褪黑素水平显著高于0及100 mg/d组(P＜0.05),总抗氧化能力显著高于0 mg/d组(P＜0.05),白细胞介素-4水平显著低于25 mg/d组(P＜0.05),白细胞介素-10水平显著低于0 mg/d组(P＜0.05)。第42天时50 mg/d组湖羊超氧化物歧化酶活性显著高于0 mg/d组(P＜0.05),白细胞介素-4水平显著低于其他各组(P＜0.05),促肾上腺皮质激素水平显著低于0及100 mg/d组(P＜0.05)。第56天时50 mg/d组湖羊精子质膜完整率显著高于0 mg/d组(P＜0.05),超氧化物歧化酶活性显著高于0 mg/d组(P＜0.05),白细胞介素-4水平显著低于0及100 mg/d组(P＜0.05)。【结论】补饲赖氨酸锌能够改善东北地区秋季湖羊种公羊血浆生殖激素水平、抗氧化能力、免疫指标水平与精子质量,建议补饲量为50 mg/d。

关键词: 赖氨酸锌; 湖羊; 精子质量; 抗氧化; 免疫

Abstract: 【Objective】 This experiment was aimed to investigate the effects of feeding zinc lysine on plasma biochemical indices and sperm quality of Hu sheep in autumn in Northeastern China.【Method】 Thirty-two 1-2 years old Hu sheep rams with well body condition were randomly divided into four groups fed with 0, 25, 50 and 100 mg/d zinc lysine, respectively.The experiment was conducted in Yichun, Heilongjiang from August to October 2022, with an average temperature of 10.3 ℃.The transition period was 7 days, and the experimental period was 56 days.On the 28th, 42nd and 56th days, the jugular vein blood of Hu sheep was collected to determine plasma testosterone, melatonin, interleukin-2, interleukin-4, interleukin-6, interleukin-10, adrenocorticotropic hormone, and tumor necrosis factor-α contents, as well as total antioxidant capacity, catalase, superoxide dismutase, glutathione peroxidase activities and malondialdehyde content.Hu sheep semen was collected to determine sperm viability, plasma membrane integrity, and abnormality rate.【Result】 On the 28th day, the melatonin content of the 50 mg/d group was significantly higher than that of the 0 and 100 mg/d groups (P＜0.05), the total antioxidant capacity was significantly higher than that of the 0 mg/d group (P＜0.05), the interleukin-4 content was significantly lower than that of the 25 mg/d group (P＜0.05), and the interleukin-10 content was significantly lower than that of the 0 mg/d group (P＜0.05).On the 42nd day, the superoxide dismutase activity of the 50 mg/d group was significantly higher than that of the 0 mg/d group (P＜0.05), the interleukin-4 content was significantly lower than that of the other groups (P＜0.05), and the adrenocorticotropic hormone content was significantly lower than that of the 0 and 100 mg/d groups (P＜0.05).On the 56th day, the sperm plasma membrane integrity rate of the 50 mg/d group was significantly higher than that of the 0 mg/d group (P＜0.05), the superoxide dismutase activity was significantly higher than that of the 0 mg/d group (P＜0.05), and the interleukin-4 content was significantly lower than that of the 0 and 100 mg/d groups (P＜0.05).【Conclusion】 Supplementing with zinc lysine could ameliorate the plasma reproductive hormones content, antioxidant capacity, immune indexes content and sperm quality of Hu sheep rams in autumn in Northeastern China.The additive amount was recommended 50 mg/d.

Key words: zinc lysine; Hu sheep; sperm quality; antioxidant; immunity

中图分类号:

S816.7

王赫川, 李天峰, 张涵, 宋宇伦, 冯赫泽, 魏姚, 程烨, 李用昕, 董莹莹, 尹国安, 郭庆, 崔卫国, 李井春. 东北地区秋季饲喂赖氨酸锌对湖羊种公羊血浆生化指标与精子质量的影响[J]. 中国畜牧兽医, 2024, 51(2): 558-567.

WANG Hechuan, LI Tianfeng, ZHANG Han, SONG Yulun, FENG Heze, WEI Yao, CHENG Ye, LI Yongxin, DONG Yingying, YIN Guoan, GUO Qing, CUI Weiguo, LI Jingchun. Effect of Feeding Zinc Lysine on Plasma Biochemical Indices and Sperm Quality of Hu Sheep Rams in Autumn in Northeastern China[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 558-567.

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