阿魏酸对脂多糖应激的吉林白鹅生长性能和肠道抗氧化指标的影响

doi:10.16431/j.cnki.1671-7236.2024.02.009

摘要/Abstract

摘要： 【目的】研究阿魏酸(FA)对脂多糖(LPS)诱导的氧化应激吉林白鹅生长性能、脏器系数以及肠道抗氧化指标的影响,为FA在鹅养殖过程中缓解氧化应激提供参考。【方法】选取7日龄健康雄性吉林白鹅120只,并随机分成6组(每组5个重复,每个重复4只):K组(空白对照组,基础饲粮)、L组(LPS对照组,基础饲粮)和A、B、C、D组(基础饲粮中分别添加60、120、180和240 mg/kg FA),在正式试验第14、17、20天时,L、A、B、C和D组腹腔注射0.5 mg/kg LPS,K组注射等体积的生理盐水,第21天时每组随机选取10只进行屠宰,取心脏、肝脏、脾脏、肾脏、法氏囊、胸腺,称重并计算脏器系数;分离十二指肠、空肠和回肠,用于测定肠道抗氧化指标。【结果】①第1~14天,与L组相比,C组的终末体重(FB)、平均日采食量(ADFI)、平均日增重(ADG)显著升高,而料重比(F/G)显著下降(P＜0.05),且除ADFI显著高于K组外,其他指标均与K组差异不显著(P＞0.05);第15~21天,与L组相比,A、B、C和D组FB、ADG、ADFI显著升高,F/G显著降低(P＜0.05),且部分指标与K组无显著差异(P＞0.05);第1~21天,与L组相比,B、C组ADG和ADFI显著升高,F/G显著下降(P＜0.05),且与K组无显著差异(P＞0.05)。②脏器系数中D组胸腺指数显著高于L和K组(P＜0.05)。③十二指肠中,A、C和D组丙二醛(MDA)含量显著低于L组(P＜0.05),且与K组相比无显著差异(P＞0.05)。④空肠中,C组MDA含量和谷胱甘肽过氧化物酶(GSH-Px)活性显著高于L组(P＜0.05),且与K组相比均无显著差异(P＞0.05);C组过氧化氢酶(CAT)活性显著高于L和K组(P＜0.05)。⑤回肠中,B和C组MDA含量显著低于L组,C组GSH-Px活性显著高于L组(P＜0.05),且均与K组差异不显著(P＞0.05)。【结论】饲粮中添加180 mg/kg 阿魏酸能够促进鹅生长,缓解各肠段氧化应激损伤。

关键词: 阿魏酸; 吉林白鹅; 脂多糖应激; 生长性能; 肠道抗氧化能力

Abstract: 【Objective】 The aim of this study was to investigate the effects of ferulic acid (FA) on the growth performance, organ indexes, and intestinal anti-oxidant indicators of Jilin White geese induced by lipopolysaccharide (LPS), and provided a reference for alleviating oxidative stress in goose breeding.【Method】 A total of 120 healthy male Jilin White geese aged 7 days were randomly divided into 6 groups (5 replicates per group, 4 geese per replicate):Group K (blank control group), group L (LPS control group), and groups A, B, C, and D (supplemented with 60, 120, 180, and 240 mg/kg FA, respectively).On days 14, 17, and 20 of the formal experiment, the geese in groups L, A, B, C, and D were injected with 0.5 mg/kg LPS intraperitoneally, while the geese in group K was injected with an equal volume of saline.On day 21, 10 geese were randomly selected from each group for slaughter, and the hearts, livers, spleens, kidneys, bursa of Fabricius, and thymuses were removed and weighed to calculate the organ indexes.The duodenum, jejunum, and ileum were separated for determination of intestinal antioxidant indicators.【Result】 ① On the 1st to 14th day, compared with the group L, the final body weight (FB), average daily feed intake (ADFI), and average daily gain (ADG) in group C were significantly increased, while the feed-to-gain ratio (F/G) was significantly decreased (P＜0.05).Except for ADFI, which was significantly higher than that of group K, other indicators in Group C were not significantly different from Group K (P＞0.05).On the 15th to 21st day, compared with group L, FB, ADG, and ADFI in groups A, B, C, and D were significantly increased, while F/G was significantly decreased (P＜0.05), and some indicators showed no significant difference compared to group K (P＞0.05).On the 1st to 21st day, compared with the group L, the ADG and ADFI in groups B and C were significantly increased, while F/G was significantly decreased (P＜0.05), and there was no significant difference compared to group K (P＞0.05).②The thymus index in group D was significantly higher than that in groups L and K in organ indexes (P＜0.05).③ In the duodenum, the content of malondialdehyde (MDA) in groups A, C, and D were significantly lower than that in group L (P＜0.05), and there was no significant difference compared to group K (P＞0.05).④ In the jejunum, MDA content and glutathione peroxidase (GSH-Px) activity in group C were significantly higher than those in group L (P＜0.05), and there was no significant difference compared to group K (P＞0.05);The activity of catalase (CAT) in group C was significantly higher than that in groups L and K (P＜0.05). ⑤In the ileum, MDA content in groups B and C was significantly lower than that in group L, and GSH-Px activity in group C was significantly higher than that in group L (P＜0.05), and there was no significant difference compared to group K (P＞0.05).【Conclusion】 The addition of 180 mg/kg ferulic acid in the diet could promote the growth of geese and alleviate oxidative stress damage in various intestinal segments.

Key words: ferulic acid; Jilin White goose; lipopolysaccharide stress; growth performance; intestinal antioxidant capacity

中图分类号:

S835

刘应昆, 郭玮, 付增宇, 孙萌, 李昊佳, 周海柱. 阿魏酸对脂多糖应激的吉林白鹅生长性能和肠道抗氧化指标的影响[J]. 中国畜牧兽医, 2024, 51(2): 521-529.

LIU Yingkun, GUO Wei, FU Zengyu, SUN Meng, LI Haojia, ZHOU Haizhu. Effects of Ferulic Acid on Growth Performance and Intestinal Anti-oxidant Indicators of Jilin White Geese Under Lipopolysaccharide Stress[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(2): 521-529.

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