产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究

doi:10.16431/j.cnki.1671-7236.2023.06.016

中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (6): 2321-2332.doi: 10.16431/j.cnki.1671-7236.2023.06.016

产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究

吴立婷¹, 陆睿^1,2, 刘半红^1,3, 包红朵¹, 王永娟⁴, 周艳¹, 王冉¹, 张辉¹

1. 江苏省农业科学院农产品质量安全与营养研究所, 江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地, 南京 210014;
2. 江苏大学食品与生物工程学院, 镇江 212013;
3. 陕西科技大学食品与生物工程学院, 西安 710021;
4. 江苏农牧科技职业学院, 泰州 225300

收稿日期:2022-11-01 出版日期:2023-06-05 发布日期:2023-05-30
通讯作者: 张辉 E-mail:Huiz@jaas.ac.cn
作者简介:吴立婷,E-mail:1206186910@qq.com;陆睿,E-mail:614230964@qq.com。
基金资助:
国家重点研发计划项目（2018YFE0101900）；江苏省重点研发计划项目（BE2022361）；江苏省农业科技自主创新资金项目（cx（21）1004）

Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers

WU Liting¹, LU Rui^1,2, LIU Banhong^1,3, BAO Hongduo¹, WANG Yongjuan⁴, ZHOU Yan¹, WANG Ran¹, ZHANG Hui¹

1. Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Institute of Food Safe and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;
3. School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China;
4. Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China

Received:2022-11-01 Online:2023-06-05 Published:2023-05-30

摘要/Abstract

摘要： 【目的】明确产气荚膜梭菌噬菌体及乳酸杆菌联用对肉鸡的生产性能、肠道形态结构及微生物多样性的影响，为禽健康养殖中产气荚膜梭菌的感染提供关键防控技术。【方法】选取240只1日龄青脚麻鸡，随机分为4组：HN02噬菌体组、乳酸杆菌组、HN02噬菌体+乳酸杆菌联用组及空白对照组，每组6个重复，每个重复10只鸡，试验期14 d，期间连续每天饮用噬菌体和/或乳酸杆菌。统计平均日采食量（ADFI）、平均日增重（ADG）及料重比（F/G），计算脏器系数。取盲肠内容物测定产气荚膜梭菌数量，并对16S rRNA基因进行扩增，分析肠道微生物群落结构和多样性。【结果】与空白对照组相比，HN02噬菌体+乳酸杆菌联用能显著提高雏鸡平均日增重及绒毛高度/隐窝深度（P<0.05），显著降低雏鸡料重比和空肠、回肠隐窝深度（P<0.05），且处理14 d的抑菌效果最佳，产气荚膜梭菌的数量减少1.17 lg CFU/mL。微生物多样性显示，HN02噬菌体+乳酸杆菌联用饮水后雏鸡盲肠内容物菌群中变形菌门、厚壁菌门、放线菌门及拟杆菌门相对丰度较高，属水平中乳杆菌属相对丰度较高，与空白对照组相比，HN02噬菌体+乳酸杆菌联用连续饮用14 d后葡萄球菌属相对丰度较低。【结论】产气荚膜梭菌噬菌体和乳酸杆菌联用能够改善肉鸡的生长性能及肠道发育，维持肠道微生物群落的多样性，在养禽业的产气荚膜梭菌感染的预防中有巨大应用潜力。

关键词: 产气荚膜梭菌; 噬菌体; 乳酸杆菌; 肉鸡; 生长性能; 肠道菌群

Abstract: 【Objective】 This study was aimed to determine the combined effects of Clostridium perfringens (C.perfringens) phage and Lactobacillus on the production performance, intestinal structure and microbial diversity of broilers, and provide the prevention and control technique for C.perfringens infection.【Method】 240 1-day-old Blue-footed partridge chickens were divided into 4 groups:HN02 phage group, Lactobacillus group, HN02 phage+Lactobacillus combination group and blank control group, with 6 replicates (10 per replicate) fed 14 days by drinking phage and/or Lactobacillus in water continuously every day during the period.The average daily food intake (ADFI), average daily gain (ADG) and feed-to-weight ratio (F/G) were counted, the organ index were measured.The number of C.perfringens of cecal content was determined and analyzed by 16S rRNA gene amplification for the structure and diversity of intestinal microbial community.【Result】 Compared with blank control group, in HN02 phage+Lactobacillus combination group, the ADG and villus height/crypt depth were significantly increased (P<0.05), the F/G and crypts depth of jejunal and ileum were significantly decreased (P<0.05), and the number of C.perfringens decreased 1.17 lg CFU/mL on 14 days with the best bacteriostatic effect.The results of microbial diversity showed that the relative abundance of Proteobacteria, Firmicutes, Actinobacteriota and Bacteroidota in the cecal content flora of broilers was higher after drinking HN02 phage+Lactobacillus, and the relative abundance of Lactobacillus was higher at the genus level.Compared with blank control group, the relative abundance of Staphylococcus was lower after drinking HN02 bacteriophage+Lactobacillus for 14 days.【Conclusion】 The combination of C.perfringens phage and Lactobacillus could improve the growth performance and intestinal development of broilers, and maintain the diversity of intestinal microbial community, which showed great potential in the prevention of C.perfringens infection in poultry industry.

Key words: Clostridium perfringens; phage; Lactobacillus; broilers; growth performance; intestinal flora

中图分类号:

S852.61

吴立婷, 陆睿, 刘半红, 包红朵, 王永娟, 周艳, 王冉, 张辉. 产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究[J]. 中国畜牧兽医, 2023, 50(6): 2321-2332.

WU Liting, LU Rui, LIU Banhong, BAO Hongduo, WANG Yongjuan, ZHOU Yan, WANG Ran, ZHANG Hui. Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2321-2332.

参考文献

[1] 李洁萍.产气荚膜梭菌的基因分型及地衣芽袍杆菌对鸡坏死胜肠炎回肠菌群的影响[D].雅安:四川农业大学, 2010. LI J P.Genetic diversity of Clostridium perfringens and effects of Bacillus licheniformi on the ileum flora of chicken necrotizing enterocolitis[D].Ya'an:Sichuan Agricultural University, 2010.(in Chinese)
[2] CHON J W, SEO K H, BAE D, et al.Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea[J].The Korean Society of Veterinary Science, 2018, 19(3):368-374.
[3] HUANG L, LI C, HWANG C A.Growth/no growth boundary of Clostridium perfringens from spores in cooked meat:A logistic analysis[J]. International Journal of Food Microbiology, 2018, 266:257-266.
[4] KORKEA L A, HAN N U, LAH T I, et al.Novel insights into the epidemiology of Clostridium perfringens type A food poisoning[J].Food Microbiology, 2011, 28:192-198.
[5] TOROK V A, HUGHES R J, MIKKELSEN L L, et al.Identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials[J].Applied & Environmental Microbiology, 2011, 77(17):5868-5878.
[6] ROSS W M, SKINNER E J, SULAKVELIDZE A, et al.Bacteriophage therapy for control of necrotic enteritis of broiler chickens experimentally infected with Clostridium perfringens[J].Avian Diseases Digest, 2010, 54(1):33-40.
[7] KIM J S, HOSSEINDOUST A, LEE S H, et al.Bacteriophage cocktail and multi-strain probiotics in the feed for weanling pigs:Effects on intestine morphology and targeted intestinal coliforms and Clostridium perfringens[J].Animal, 2017, 1(1):45-53.
[8] BAE D, LEE J W, CHAE J P, et al.Characterization of a novel bacteriophage φCJ22 and its prophylactic and inhibitory effects on necrotic enteritis and Clostridium perfringens in broilers[J].Poultry Science, 2021, 100(1):302-313.
[9] KIM K P, BORN Y, LURZ R, et al.Inducible Clostridium perfringens bacteriophages ΦS9 and ΦS63[J].Bacteriophage, 2014, 2(2):89-97.
[10] SEAL B S, FOUTS D E, SIMMONS M, et al.Clostridium perfringens bacteriophages ΦCP39O and ΦCP26F:Genomic organization and proteomic analysis of the virions[J].Archives of Virology, 2011, 156(1):25-35.
[11] GUO S S, LIU D, ZHANG B B, et al.Two Lactobacillus species inhibit the growth and a-toxin production of Clostridium perfringens and induced proinflammatory factors in chicken intestinal epithelial cells in vitro[J].Frontiers in Microbiology, 2017, 8:2081.
[12] KEYBURN A L, BANNAM T L, MOORE R J, et al.NetB, a pore-forming toxin from necrotic enteritis strains of Clostridium perfringens[J].Toxins, 2010, 2(7):1913-1927.
[13] 罗涛, 祁姣姣, 彭玉莉, 等.对产气荚膜梭菌具有抑菌作用的益生菌的筛选及其抑菌性能探究[J].饲料研究, 2021, 44(8):83-87. LUO T, QI J J, PENG Y L, et al.Screening of probiotics with bacteriostatic effect on Clostridium perfringens and their bacteriostatic properties[J].Feed Research, 2021, 44(8):83-87.(in Chinese)
[14] YUAN T, LW B, RUI L, et al.Virulent phage vB_CpeP_HN02 inhibits Clostridium perfringens on the surface of the chicken meat[J].International Journal of Food Microbiology, 2022, 363:109514.
[15] 胡军, 孙毅, 郑江平, 等.基于Illumina Miseq高通量测序技术分析幼龄比格犬肠道菌群多样性[J].黑龙江畜牧兽医, 2022, 11:90-93. HU J, SUN Y, ZHENG J P, et al.Analysis of gut microbiota diversity in young Beagle dogs based on Illumina Miseq high-throughput sequencing technology[J].Heilongjiang Animal Science and Veterinary Medicine, 2022, 11:90-93.(in Chinese)
[16] 雷英, 苏凯, 王运盛, 等.基于高通量测序技术对不同季节圈养褐马鸡肠道菌群多样性分析[J].野生动物学报, 2022, 43(4):990-998. LEI Y, SU K, WANG Y S, et al.Changes in intestinal microflora diversity of captive Brown eared pheasants among different seasons using high-throughput sequencing[J].Chinese Journal of Wildlife, 2022, 43(4):990-998.(in Chinese)
[17] 葛龙, 贺永超, 董强, 等.噬菌体制剂对肉鸡生长性能, 肠道菌群及免疫功能的影响[J].饲料研究, 2020, 45(4):111-115. GE L, HE Y C, DONG Q, et al.Effect of bacteriophage preparation on growth performance, intestinal floraand immune organs of broilers[J].Feed Research, 2020, 45(4):111-115.(in Chinese)
[18] KIM M S, BAE J W.Spatial disturbances in altered mucosal and luminal gut viromes of diet-induced obese mice[J].Environmental Microbiology, 2016, 18(5):1498-1510.
[19] 周贝, 林焱, 朱伟云.肠道噬菌体与细菌和宿主互作及其对动物机体健康影响的研究进展[J].畜牧兽医学报, 2019, 50(1):14-20. ZHOU B, LIN Y, ZHU W Y.Advances in the interaction among gut phage, bacteria and host and its impacts on the animal host health[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(1):14-20.(in Chinese)
[20] 朱树娇, 张辉, 周艳, 等.噬菌体复合制剂对肉鸡生长性能, 免疫功能, 肠道形态及肠道菌群的影响[J].动物营养学报, 2022, 34(5):2928-2939. ZHU S J, ZHANG H, ZHOU Y, et al.Effects of phage cocktail preparation on growth performance, immune function, intestinal morphology and gut microbiota of broilers[J].Chinese Journal of Animal Nutrition, 2022, 34(5):2928-2939.(in Chinese)
[21] KIM J S, INGALE S L, KIM Y W, et al.Effect of supplementation of multi-microbe probiotic product on growth performance, apparent digestibility, cecal microbiota and small intestinal morphology of broilers[J].Journal of Animal Physiology and Animal Nutrition, 2012, 96(4):618-626.
[22] CHOI J Y, KIM J S, INGALE S L, et al.Effect of potential multimicrobe probiotic product processed by high drying temperature and antibiotic on performance of weanling pigs[J].Journal of Animal Science, 2011, 89(6):1795-1804.
[23] CHOI J Y, SHINDE P L, INGALE S L, et al.Evaluation of multi-microbe probiotics prepared by submerged liquid or solid substrate fermentation and antibiotics in weaning pigs[J].Livestock Science, 2011, 138(1-3):144-151.
[24] MOURAO J L, PINHEIRO V, ALVES A, et al.Effect of mannan oligosaccharides on the performance, intestinal morphology and cecal fermentation of fattening rabbits[J].Animal Feed Science and Technology, 2005, 126:107-120.
[25] SAMANYA M, YAMAUCHI K.Histological alterations of intestinal villi in chickens fed dried Bacillus subtilis var.natto[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology, 2002, 133(1):95-104.
[26] 李成良.饲粮芽孢杆菌调控肉鸡肠道屏障的作用机制[D].长沙:湖南农业大学, 2010. LI C L.Effects of dietary Bacillus spp.supplementation on intestinal barrier of broiler chicks[D].Changsha:Hunan Agricultural University, 2010.(in Chinese)
[27] LI C L, WANG J, ZHANG H J, et al.Intestinal morphologic and microbiota responses to dietary Bacillus spp.in a broiler chicken model[J].Frontiers in Physiology, 2019, 9:1968.
[28] 张灿, 卢国民, 刘文华, 等.灌服噬菌体对白羽肉鸡肠道菌群的影响[J].中国抗生素杂志, 2017, 42(9):755-759. ZHANG C, LU G M, LIU W H, et al.Effects of bacteriophages on intestinal microbiota of White feather broilers by oral administration[J]. Chinese Journal of Antibiotics, 2017, 42(9):755-759.(in Chinese)
[29] VALERIA A T, ROBERT J H, LENE L M, et al.Identification and characterization of potential performance-related gut microbiotas in broiler chickens across various feeding trials[J].Applied & Environmental Microbiology, 2011, 77(17):5868-5878.
[30] 卢国民, 张灿, 刘军伟, 等.大肠杆菌噬菌体对肉鸡肠道菌群的影响[J].中国兽医学报, 2017, 37(5):833-838. LU G M, ZHANG C, LIU J W, et al.Effects of coliphages on the intestinal microflora of broilers[J].Chinese Journal of Veterinary Medicine, 2017, 37(5):833-838.(in Chinese)
[31] 崔磊.不同性别鸡肠道菌群差异的比较研究[D].武汉:华中农业大学, 2019. CUI L.Comparative study on the gut microbiota differ ences of chickens with different gender[D].Wuhan:Huazhong Agricultural University, 2019.(in Chinese)
[32] HUANG P, ZHANG Y, XIAO K P, et al.The chicken gut metagenome and the modulatory effects of plant-derived benzylisoquinoline alkaloids[J].Microbiome, 2018, 6(1):211.
[33] SALARMOINI M, FOOLADI M H.Efficacy of Lactobacillus acidophilus as probiotic to improve broiler chicks performance[J].Journal of Agricultural Science and Technology, 2011, 13(2):165-172.
[34] HSU B B, GIBSON T E, YELISEYEV V, et al.Dynamic modulation of the gut microbiota and metabolome by bacteriophages in a mouse model[J].Cell Host Microbe, 2019, 25:803-814.
[35] MAHANA D, TRENT C M, KURTZ Z D, et al.Antibiotic perturbation of the murine gut microbiome enhances the adiposity, insulin resistance, and liver disease associated with high-fat diet[J].Genome Medicine, 2016, 8(1):48.
[36] CHARBONNEAU M, O'DONNELL D, BLANTON L, et al.Sialylated milk oligosaccharides promote microbiota-dependent growth in models of infant undernutrition[J].Cell, 2016, 164(5):859-871.
[37] 陆建强, 蔡猛, 聂根基, 等.枯草芽孢杆菌与地顶孢霉复合制剂对蛋鸡生产性能、蛋品质及肠道相关指标的影响[J].畜牧与兽医, 2021, 53(11):21-25. LU J Q, CAI M, NIE G J, et al.Combined flects of Bacillus subtilis and Acremonium on the performance, egg quality and related intestinal indexes in laying hens[J].Animal Husbandry & Veterinary Medicine, 2021, 53(11):21-25.(in Chinese)
[38] YAN L, HONG S M, KIM I H.Effect of bacteriophage supplementation on the growth performance, nutrient digestibility, blood characteristics, and fecal microbial shedding in growing pigs[J]. Asian-Australasian Journal of Animal Sciences, 2012, 25(10):1451-1456.
[39] KIM J H, KIM J W, LEE B B, et al.Effect of dietary supplementation of bacteriophage on growth performance and cecal bacterial populations in broiler chickens raised in different housing systems[J].Livestock Science, 2014, 170:137-141.

产气荚膜梭菌噬菌体及乳酸杆菌联用改善肉鸡生长性能和调节肠道菌群功能研究

Combination of Clostridium perfringens Phage and Lactobacillus Improves Growth Performance and Regulates Intestinal Flora Function of Broilers

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