蚯蚓液对13~18周龄蛋鸡生长性能、血清生化、抗氧化及免疫指标的影响

doi:10.16431/j.cnki.1671-7236.2023.12.010

摘要/Abstract

摘要： 【目的】探究饲喂不同比例蚯蚓液对蛋鸡生长性能、血清生化指标、抗氧化指标及免疫指标的影响。【方法】选取12周龄末体重相近、健康良好的海兰粉蛋鸡432只,随机分为4组,每组6个重复,每个重复18只,对照组饲喂基础饲粮,试验组分别饲喂添加1%、2%和3%蚯蚓液的基础饲粮,试验期为6周。试验期间记录每日采食量;测定13和18周龄蛋鸡的体重;18周龄时,每个重复选取6只蛋鸡采集血清,用于测定血清生化指标、抗氧化指标和免疫指标。【结果】与对照组相比,①各试验组蛋鸡生长性能指标差异均不显著(P＞0.05);②饲粮中添加1%和3%蚯蚓液显著降低蛋鸡血清白球比(A/G)(P＜0.05),显著提高蛋鸡血清总蛋白(TP)水平(P＜0.05);添加3%蚯蚓液显著提高蛋鸡的血清球蛋白(GLB)水平(P＜0.05));③饲粮中添加1%、2%和3%蚯蚓液均极显著提高蛋鸡血清总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化氢酶(GSH-Px)活力(P＜0.01);添加1%蚯蚓液极显著降低蛋鸡血清丙二醛(MDA)含量(P＜0.01);④饲粮中添加1%蚯蚓液极显著提高蛋鸡血清免疫球蛋白G(IgG)水平(P＜0.01),显著提高蛋鸡血清IgM水平(P＜0.05);添加1%、2%和3%蚯蚓液均极显著降低蛋鸡血清白细胞介素-2(IL-2)和IL-6水平(P＜0.01);添加1%蚯蚓液极显著降低蛋鸡血清IL-17水平(P＜0.01);添加1%和2%蚯蚓液极显著降低蛋鸡血清肿瘤坏死因子α(TNF-α)水平(P＜0.01)。【结论】饲粮中添加蚯蚓液在不影响蛋鸡生长性能的基础上,可以提高蛋鸡的抗氧化能力和免疫功能,以1%的添加量为宜。

关键词: 蚯蚓液; 蛋鸡; 生长性能; 抗氧化能力; 免疫功能

Abstract: 【Objective】 To study the effects of earthworm hydrolysate on growth performance,serum biochemical,antioxidant,and immune indices of laying hens.【Method】 A total of 432 healthy 12-weeks old Hy-line Pink laying hens with similar body weight were randomly divided into 4 groups with 6 replicates per group and 18 hens per replicate.Hens in control group were fed a basal diet,and hens in experimental groups were fed a basal diet supplemented with 1%,2% and 3% earthworm hydrolysate,respectively.The experiment lasted for 6 weeks.During the experiment period,daily feed intake was recorded daily and weighted at 13th and 18th weeks old.At 18th weeks old six laying hens from each replicate were randomly selected to collect serum for the determination of serum biochemical indices,antioxidant indices and immune indices.【Result】 Compared with the control group,①The differences in the growth performance indexes of laying hens of each test group were not significant (P＞0.05).②Diets supplemented with 1% and 3% earthworm hydrolysate significantly decreased the serum albumin/globulin (A/G) (P＜0.05), and significantly increased the level of total protein (TP) of laying hens (P＜0.05).Supplemented with 3% earthworm hydrolysate significantly increased the level of globulin (GLB) of laying hens (P＜0.05).③Diet supplemented with 1%,2%,and 3% earthworm hydrolysate extremely significantly increased the activities of serum total antioxidant capacity (T-AOC),superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of laying hens (P＜0.01).Supplemented with 1% earthworm hydrolysate significantly decreased the level of serum malondialdehyde (MDA) level of laying hens (P＜0.01).④Diet supplemented with 1% earthworm hydrolysate extremely increased the level of serum immunoglobulin G (IgG) (P＜0.01),and significantly increased the level of serum IgM of laying hens (P＜0.05).Supplemented with 1%,2%,and 3% earthworm hydrolysate significantly decreased the levels of serum interleukin-2 (IL-2) and IL-6 of laying hens (P＜0.01).Supplemented with 1% earthworm hydrolysate significantly decreased the levels of serum IL-17 of laying hens (P＜0.01).Supplemented with 1% and 2% earthworm hydrolysate significantly decreased the level of serum tumor necrosis factor α (TNF-α) of laying hens (P＜0.01).【Conclusion】 On the basis of without affecting growth performance,diet supplemented with earthworm hydrolysate could enhance antioxidant capacity and immune function of laying hens,and the optimum dosage was 1%.

Key words: earthworm hydrolysate; laying hens; growth performance; antioxidant capacity; immune function

中图分类号:

S816.75

成笛, 谢华亮, 钟云平, 刘珍妮, 连海, 谭东海, 雷小文. 蚯蚓液对13~18周龄蛋鸡生长性能、血清生化、抗氧化及免疫指标的影响[J]. 中国畜牧兽医, 2023, 50(12): 4876-4885.

CHENG Di, XIE Hualiang, ZHONG Yunping, LIU Zhenni, LIAN Hai, TAN Donghai, LEI Xiaowen. Effects of Earthworm Hydrolysate on Growth Performance,Serum Biochemical, Antioxidant,and Immune Indices of Laying Hens Aged 13 to 18 Weeks[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(12): 4876-4885.

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