补饲胆固醇对高温环境下湖羊母羊繁殖性能和血清生化指标的影响

doi:10.16431/j.cnki.1671-7236.2024.01.015

摘要/Abstract

摘要： 【目的】研究饲粮中添加胆固醇对中国南方夏季湖羊发情率、受胎率和血清生化指标的影响。【方法】选择108只体重相近（33.60 kg±1.86 kg）的健康湖羊母羊，其中30只湖羊在非高温季节（4月份，平均最高温度20.9 ℃±3.2 ℃）饲粮添加不同剂量胆固醇（0.1%和0.5%），ELISA方法检测血清中胆固醇含量的动态变化，筛选合适的胆固醇添加量；另外78只湖羊随机分成2组（对照组和试验组），试验期63 d （从同期发情处理第1天到自然发情后配种的第30天），在夏季高温季节（7－9月份，平均最高温度31.3 ℃±2.6 ℃），对照组饲喂基础饲粮，试验组饲喂添加0.1%胆固醇的基础饲粮，ELISA方法检测血清中雌二醇（E₂）和孕酮（P₄）水平，研究饲粮添加胆固醇对湖羊发情率、受胎率和血清生化指标的影响。【结果】非高温季节湖羊基础饲粮中分别添加0.1%和0.5%胆固醇，48 h后两组湖羊血清中胆固醇含量趋于一致，据此选择0.1%作为高温季节补饲胆固醇的添加剂量。夏季高温季节在补饲0.1%胆固醇的第3、30和63天均能显著提高湖羊血清中胆固醇含量（P<0.05）；且试验组湖羊自然发情率（89.7%）高于对照组（82.1%）（P>0.05），受胎率（60.0%）显著高于对照组（46.9%）（P<0.05）。试验组湖羊总蛋白、球蛋白、白细胞介素-1和甘油三酯含量均显著高于对照组（P<0.05），而尿素氮含量显著下降（P<0.05）。【结论】夏季高温环境下，在湖羊母羊饲粮中添加0.1%胆固醇可有效提高其发情率和受胎率。

关键词: 湖羊; 高温环境; 胆固醇; 发情率; 血清指标

Abstract: 【Objective】 The aim of this study was to investigate the effect of cholesterol supplementation on estrus rate, conception rate and serum biochemical indexes of Hu sheep in southern summer of China.【Method】 108 healthy Hu sheep ewes with similar body weight (33.60 kg±1.86 kg) were selected, and 30 ewes were fed with different doses of cholesterol (0.1% and 0.5%) and detect the dynamic changes of serum cholesterol content in April (non high temperature state, average highest temperature is 20.9 ℃±3.2 ℃) to select the appropriate dose of cholesterol. Another 78 Hu sheep were randomly divided into two groups, and the experimental period was 63 days (from the first day of estrus synchronization to the 30th day of mating after natural estrus), the ewes in control group were fed with basic diet, and the ewes in experimental group were fed with basic diet supplemented with cholesterol from July to September (high temperature state, average highest temperature is 31.3 ℃±2.6 ℃), and the effects of dietary cholesterol on the estrus rate, conception rate and serum biochemical indexes of Hu sheep were studied.【Result】 No matter whether 0.1% or 0.5% cholesterol was supplemented to the basic diet under non high temperature state, the cholesterol content in serum of the two groups tended to be the same after 48 h, so 0.1% cholesterol was selected for the supplementary feeding experiment in the diet of high temperature environment.Supplemental feeding of 0.1% cholesterol on the 3rd, 30th and 63rd days could significantly increase the serum cholesterol content of Hu sheep, and the natural estrus rate (89.7%) of the experimental group were higher than those of the control group (82.1%) (P>0.05), the conception rate of the experimental group(60.0%) was significantly higher than that of the control group (46.9%)(P<0.05).The content of total protein (TP), globulin (GLB), IL-1 and triglyceride (TG) in the experimental group were significantly higher than those in the control group (P<0.05), while the content of urea nitrogen was significantly decreased (P<0.05).【Conclusion】 Adding 0.1% cholesterol to the diet of Hu sheep ewes in high temperature season could enhance its estrus rate and conception rate.

Key words: Hu sheep; high temperature environment; cholesterol; estrus rate; serum indexes

中图分类号:

S816.7

李隐侠, 舒嘉傲, 张晨俭, 李樊, 钱勇, 孟春花, 张俊, 张建丽, 曹少先. 补饲胆固醇对高温环境下湖羊母羊繁殖性能和血清生化指标的影响[J]. 中国畜牧兽医, 2024, 51(1): 145-152.

LI Yinxia, SHU Jiaao, ZHANG Chenjian, LI Fan, QIAN Yong, MENG Chunhua, ZHANG Jun, ZHANG Jianli, CAO Shaoxian. Effects of Cholesterol Supplementation on Reproductive Performance and Serum Biochemical Indexes in Hu Sheep Ewes under High Temperature Environment[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(1): 145-152.

参考文献

[1] VAN WETTERE W J, KIND K L, GATFORD K L, et al.Review of the impact of heat stress on reproductive performance of sheep[J].Journal of Animal Science and Biotechnology, 2021, 12(1):26.
[2] ROMO-BARRON C B, DIAZ D, PORTILLO-LOERA J J, et al.Impact of heat stress on the reproductive performance and physiology of ewes:A systematic review and meta-analyses[J].International Journal of Biometeorology, 2019, 63(7):949-962.
[3] KLEEMANN D O, WALKER S K.Fertility in south Australian commercial Merino flocks:Relationships between reproductive traits and environmental cues[J].Theriogenology, 2005;63(9):2416-2433.
[4] RISPOLI L A, EDWARDS J L, POHLER K G, et al.Heat-induced hyperthermia impacts the follicular fluid proteome of the periovulatory follicle in lactating dairy cows[J].PLoS One, 2019, 14(12):e0227095.
[5] BALDWIN R L, SMITH N E, TAYLOR J, et al.Manipulating metabolic parameters to improve growth rate and milk secretion[J].Journal of Animal Science, 1980, 51(6):1416-1428.
[6] KUMAR D, DE K, SHEKHAWAT I, et al.Combined effect of heat and nutritional stress on superovulation of Maplura ewes in a semi-arid region[J].Journal of Thermal Biology, 2019, 80:158-163.
[7] 施力光, 彭维祺, 胡海超, 等.持续性环境热应激对公羊血液生化指标、生殖激素及精液品质的影响[J].家畜生态学报, 2018, 39(3):53-57. SHI L G, PENG W Q, HU H C, et al.Effect of long-term heat stress on blood biochemical and physiological index, hormone, and semen quality of goat[J].Acta Ecologiae Animalis Domastici, 2018, 39(3):53-57.(in Chinese)
[8] LUO J, YANG H, SONG B L.Mechanisms and regulation of cholesterol homeostasis[J].Nature Reviews Molecular Cell Biology, 2020, 21(4):225-245.
[9] LI F K, YANG Y, JENNA K, et al.Effect of heat stress on the behavioral and physiological patterns of small-tail Han sheep housed indoors[J].Tropical Animal Health and Production, 2018, 50(8):1893-1901.
[10] RATHWA S D, VASAVA A A, PATHAN M M, et al.Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep[J].Vet World, 2017, 10(6):650-654.
[11] FORETZ M, ANCELLIN N, ANDREELLI F, et al.Short-term overexpression of a constitutively active form of AMP-activated protein kinase in the liver leads to mild hypoglycemia and fatty liver[J].Diabetes, 2005, 54(5):1331-1339.
[12] KANG H, OH T, BAHK Y Y, et al.HSF1 regulates mevalonate and cholesterol biosynthesis pathways[J].Cancers (Basel), 2019, 11(9):1363.
[13] TAO S, ORELLANA RIVAS R M, MARINS T N, et al.Impact of heat stress on lactational performance of dairy cows[J].Theriogenology, 2020, 150:437-444.
[14] 彭孝坤, 赵天, 黄晓瑜, 等.急性热应激对山羊血液生化指标及血淋巴细胞热休克蛋白70家族基因表达的影响[J].畜牧兽医学报, 2019, 50(6):1219-1229. PENG X K, ZHAO T, HUANG X Y, et al.Effects of acute heat stress on blood biochemistry indices and expression of HSP70 family genes in blood lymphocytes in goats[J].Acta Veterinaria et Zootechnica Sinica, 2019, 50(6):1219-1229.(in Chinese)
[15] KARTHIK D, SURESH J, REDDY Y R, et al.Farming systems in sheep rearing:Impact on growth and reproductive performance, nutrient digestibility, disease incidence and heat stress indices[J].PLoS One, 2021, 16(1):e0244922.
[16] DUTT R H.Detrimental effects of high ambient temperature on fertility and early embryo survival in sheep[J].International Journal of Biometeorology, 1964, 8(1):47-56.
[17] GARCIA-ISPIERTO I, ABDELFATAH A, LÓPEZ-GATIUS F.Melatonin treatment at dry-off improves reproductive performance postpartum in high-producing dairy cows under heat stress conditions[J].Reproduction in Domestic Animals, 2013, 48(4):577-583.
[18] ABECIA J A, FORCADA F, ZUNIGA O.The effect of melatonin on the secretion of progesterone in sheep and on the development of ovine embryos in vitro[J].Veterinary Research Communications, 2002, 26(2):151-158.
[19] BOUROUTZIKA E, KOURETAS D, PAPADOPOULOS S, et al.Effects of melatonin administration to pregnant ewes under heat-stress conditions, in redox status and reproductive outcome[J].Antioxidants (Basel), 2020, 9(3):266.
[20] ARECHIGA C F, STAPLES C R, MCDOWELL L R, et al.Effects of timed insemination and supplemental beta-carotene on reproduction and milk yield of dairy cows under heat stress[J].Journal of Dairy Science, 1998, 81(2):390-402.
[21] IKEDA S, KITAGAWA M, IMAI H, et al.The roles of vitamin A for cytoplasmic maturation of bovine oocytes[J].Journal of Reproduction and Development, 2005, 51(1):23-35.
[22] LI Y X, GUO X Z, GULAPPA T, et al.SREBP play a regulatory role in LH/hCG receptor mRNA expression in human granulosa-lutein cells[J].Journal of Clinical Endocrinology Metabolism, 2019, 104(10):4783-4792.
[23] SCHOKNECHT P A, EBNER S, POND W G, et al.Dietary cholesterol supplementation improves growth and behavioral response of pigs selected for genetically high and low serum cholesterol[J].Nutrient Requirements and Interactions, 1994, 124(2):305-314.
[24] LI P, KIM S W, LI X L, et al.Dietary supplementation with cholesterol and docosahexaenoic acid affects concentrations of amino acids in tissues of young pigs[J].Amino Acids, 2009, 37:709-716.
[25] 满敦蕊, 袁融, 张禹熙, 等.饲料中添加胆固醇对中华绒螯蟹幼蟹生长、蜕皮及亚硝酸盐应激前后抗氧化能力的影响[J].饲料工业, 2023, 44(10):67-73. MAN D R, YUAN R, ZHANG Y X, et al.Effects of dietary cholesterol addition level on growth, molting, cholesterol content and antioxidant capability of juvenile Chinese Mitten crab(Eriocheir sinensis) before and after nitrite exposure[J].Feed Industry, 2023, 44(10):67-73.(in Chinese)
[26] GONZALEZ-RIVAS P A, CHAUHAN S S, HA M, et al.Effects of heat stress on animal physiology, metabolism, and meat quality:A review[J].Meat Science, 2020, 162:108025.
[27] BAGATH M, KRISHNAN G, DEVARAJ C, et al.The impact of heat stress on the immune system in dairy cattle:A review[J].Research in Veterinary Science, 2019, 126:94-102.
[28] 周顺伍.动物生物化学[M].北京:中国农业出版社, 2008. ZHOU S W.Animal Biochemistry[M].Beijing:China Agriculture Press, 2008.(in Chinese)
[29] SAKER K E, FIKE J H, VEIT H, et al.Brown seaweed-(Tasco) treated conserved forage enhances antioxidant status and immune function in heat-stressed wether lambs[J].Journal of Animal Physiology Animal Nutrition (Berl), 2004, 88:122-130.
[30] 汪水平, 王文娟, 左福元, 等.中药复方对夏季肉牛的影响:Ⅱ.血气指标、血清代谢产物浓度及免疫和抗氧化功能参数[J].畜牧兽医学报, 2011, 42(5):734-741. WANG S P, WANG W J, ZUO F Y, et al.Effect of Chinese medicine prescription on beef cattle in summer:Ⅱ.The blood gas analysis, the concentration of metabolites and the parameters of the immune and antioxidant capability in the serum[J].Acta Veterinaria et Zootechnica Sinica, 2011, 42(5):734-741.(in Chinese)
[31] 刘圈炜, 卢庆萍, 张宏福, 等.持续高温对生长猪血清生化指标及肌肉营养物质含量的影响[J].动物营养学报, 2010, 22(5):1207-1213. LIU Q W, LU Q P, ZHANG H F, et al.Effects of sustained high ambient temperature on serum biochemical parameters and muscle nutrient contents of growing pigs[J].Chinese Journal of Animal Nutrition, 2010, 22(5):1207-1213.(in Chinese)
[32] 张红瑞, 高燕, 闫灵敏, 等.α-硫辛酸对热应激绵羊生长性能、抗氧化及免疫的影响[J].动物营养学报, 2019, 31(4):1754-1764. ZHANG H R, GAO Y, YAN L M, et al.Effects of α-lipoic acid on growth performance, antioxidant and immunity of sheep under heat stress[J].Chinese Journal of Animal Nutrition, 2019, 31(4):1754-1764. (in Chinese)
[33] 汪水平, 王文娟, 谭支良.反刍动物体内氮与尿酸代谢研究进展[J].西北农林科技大学学报(自然科学版), 2009, 37(2):62-72. WANG S P, WANG W J, TAN Z L.Research advance on ammonia and urea metabolism for ruminants[J].Journal of Northwest A&F University (Natural Science Edition), 2009, 37(2):62-72.(in Chinese)