乌湖杂交羊GnRHR与INHA基因多态性及其互作效应对产羔数的影响

doi:10.16431/j.cnki.1671-7236.2020.03.016

摘要/Abstract

摘要： 试验旨在探究GnRHR和INHA基因遗传变异及其互作效应对绵羊产羔数的影响。参考GenBank发布的绵羊GnRHR基因（登录号：AH004943）和牛INHA基因（登录号：U16237）的DNA序列设计引物，采用PCR-SSCP技术检测GnRHR和INHA基因在乌湖羊、湖羊、乌骨绵羊中的遗传多态性，分析多态位点与产羔数的相关性，以及GnRHR和INHA基因间互作效应。结果显示，乌湖羊和湖羊群体GnRHR和INHA基因均存在多态位点，分别检测到GG、GC、CC和AA、AB、BB基因型，乌骨绵羊因样本较少，未发现多态位点。测序发现，GnRHR基因编码区198 bp处发生G→C突变，导致甘氨酸变为精氨酸，为错义突变；INHA基因877 bp处发生T→C突变，为同义突变（仍为天冬氨酸）。遗传学分析显示，GG和AA为优势基因型，G和A为优势等位基因；χ²适合性检验显示，试验羊群体GnRHR和INHA基因型分布处于Hardy-Weinberg平衡（P>0.05）；分析GnRHR与INHA基因互作效应发现，基因互作效应在乌湖羊和湖羊群体差异显著（P<0.05），ABCC互作基因型互作效应最大，AAGG互作基因型互作效应最小。乌湖羊ABCC互作基因型的平均产羔数比AAGG互作基因型多1.68只，湖羊ABCC互作基因型产羔数比AAGG互作基因型多1.32只，ABCC互作基因型比AB和CC基因型的产羔数都高，表明互作效应与羊产羔数呈正相关。本研究认为，INHA基因T877C位点与GnRHR基因G198C位点互作基因型与绵羊产羔数紧密关联，对试验羊群体产羔数影响显著。

关键词: 乌湖羊; GnRHR基因; INHA基因; PCR-SSCP; 产羔性状; 互作效应

Abstract: This study was aimed to analyze the influence of genetic variation of GnRHR and INHA genes and its interaction effect on the number of lambing in sheep.Primers were designed according to DNA sequence of GnRHR and INHA genes (GenBank accession No.:AH004943 and U16237).PCR-SSCP was used to detect the genetic polymorphisms of GnRHR and INHA genes in Wuhu sheep,Hu sheep and Black bone sheep,the correlation between polymorphic loci and litter size and the interaction effect between GnRHR and INHA genes were analyzed.The results showed that AA,AB and BB genotypes,GG,GC and CC genotypes were detected in GnRHR and INHA genes of Wuhu sheep and Hu sheep,no polymorphism site was found in Black bone sheep with few samples.G→C mutation occurred at 198 bp of GnRHR gene CDS,resulting in glycine to arginine,which was missense mutation;T→C mutation occurred at 877 bp of INHA gene,which was synonymous mutation (still aspartic acid).Genetic analysis results showed that GG and AA were dominant genotypes,and G and A were dominant alleles.The Chi-square test results showed that GnRHR and INHA genotypes distribution was in Hardy-Weinberg equilibrium (P>0.05).By analyzing the interaction effect between GnRHR and INHA genes,it was found that there was a significant difference in the interaction effect between Wuhu and Hu sheep (P<0.05).The interaction effect of ABCC genotype was the largest,and AAGG genotype was the smallest.The average lambing number of ABCC genotype were 1.68 and 1.32 in Wuhu sheep and Hu sheep,respectively,which was higher than that of AAGG genotype,and the lambing number of ABCC genotype was higher than that of AB and CC genotypes,which indicated that the interaction effect was positively correlated with lambing number.This results suggested that interaction genotype of INHA (T877C) and GnRHR (G198C) was closely related to the lambing number of sheep,which had a significant effect on the litter size in sheep.

Key words: Wuhu sheep; GnRHR gene; INHA gene; PCR-SSCP; litter size trait; interactive effect

中图分类号:

S813.3

刘海霞, 韩大勇, 朱爱文, 闫伟, 王步忠, 蔡子健. 乌湖杂交羊GnRHR与INHA基因多态性及其互作效应对产羔数的影响[J]. 中国畜牧兽医, 2020, 47(3): 780-788.

LIU Haixia, HAN Dayong, ZHU Aiwen, YAN Wei, WANG Buzhong, CAI Zijian. Effect of the Polymorphism of GnRHR and INHA Genes and Its Interaction on Litter Size in Wuhu Hybrid Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(3): 780-788.

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