不同浓度芦丁和冷冻速率对猪精子冷冻保存效果的研究

doi:10.16431/j.cnki.1671-7236.2022.03.020

摘要/Abstract

摘要： 【目的】探讨冷冻稀释液中添加不同浓度芦丁和不同冷冻速率对杜洛克公猪精子冷冻保存效果的影响及其二者的互作关系，以期为指导生产实践和提高优良种猪利用率提供依据。【方法】试验分为6组，分别为空白对照组（冷冻稀释液Ⅰ液）和试验组（分别在冷冻稀释液Ⅰ液中添加0.2、0.4、0.6、0.8和1.0 mmol/L芦丁），在距离液氮面上1 cm （快冷冻）和3 cm （慢冷冻）处分别进行冷冻。在液氮中保存30 d后，检测冷冻-解冻精子的运动参数、质膜完整率（MI）、顶体完整率（AI）、DNA完整率、线粒体膜电位（MMP）、活性氧（ROS）水平、丙二醛（MDA）和ATP含量，以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的活性来评价解冻后的精液品质。【结果】冷冻速率方面，在相同浓度芦丁冷冻液中，慢冷冻效果均好于快冷冻效果，差异显著（P<0.05）。芦丁浓度方面，快冷冻和慢冷冻组均以添加0.6 mmol/L芦丁的效果最好（P<0.05），添加0.8 mmol/L芦丁的效果次之。二者互作方面，冷冻速率与芦丁浓度间存在交互作用，其中慢冷冻×0.6 mmol/L芦丁组合的精子的运动参数、质膜完整率、顶体完整率、DNA完整率、线粒体膜电位、ATP含量均显著高于其他组合试验组（P<0.05），ROS水平显著低于其他组合试验组（P<0.05），SOD、CAT和GSH-Px的活性较其他组合试验组均显著提高（P<0.05）。【结论】不同芦丁浓度与不同冷冻速率间存在互作效应，其中在冷冻稀释液中添加0.6 mmol/L芦丁慢冷冻猪精子效果最好。

关键词: 芦丁; 猪; 冷冻速率; 精液品质; 互作效应

Abstract: 【Objective】 The purpose of this study was to investigate the effects of different concentrations of rutin and different freezing rates on the cryopreservation of Duroc boar sperm and their interaction, in order to provide basis for guiding production practice and improving utilization rate of excellent breeding pigs.【Method】 The experiment was divided into 6 groups:Blank control group (frozen diluent solution Ⅰ), and experimental groups (0.2, 0.4, 0.6, 0.8 and 1.0 mmol/L rutin were added to the frozen diluent solution Ⅰ, respectively).Semen were frozen at 1 cm (fast freezing) and 3 cm (slow freezing) above the liquid nitrogen surface, respectively.After being stored in liquid nitrogen for 30 days, sperm motility parameters, membrane integrity rate (MI), acrosome integrity rate (AI), mitochondrial membrane potential(MMP), DNA integrity rate, ROS level, MDA and ATP content, and the activities of SOD, CAT and GSH-Px were measured to evaluate the frozen-thawed semen quality.【Result】 In terms of freezing rate, in the same concentration of rutin freezing solution, the slow freezing effect was better than the fast freezing effect, and the difference was significant (P<0.05).In terms of rutin concentration, the effect of adding 0.6 mmol/L rutin was the best (P<0.05), followed by 0.8 mmol/L.In terms of their interaction, there was an interaction between freezing rate and rutin concentration, especially sperm motility parameters, membrane integrity, acrosome integrity, DNA integrity, mitochondrial membrane potential and ATP content of slow freezing×0.6 mmol/L rutin combination were significantly higher than those of other combination experimental groups (P<0.05), and the level of ROS was significantly lower than that of other combination experimental groups(P<0.05).The activities of SOD, CAT and GSH-Px were significantly increased compared with other groups.【Conclusion】 There were interaction effects between different rutin concentrations and different freezing rates, and adding 0.6 mmol/L rutin into the slow freezing diluent had the best effect.

Key words: rutin; boar; freezing rate; semen quality; interaction effect

中图分类号:

S828

罗兰, 崔超, 董瑞兰, 刘浩域, 柳小斌, 于光辉. 不同浓度芦丁和冷冻速率对猪精子冷冻保存效果的研究[J]. 中国畜牧兽医, 2022, 49(3): 982-991.

LUO Lan, CUI Chao, DONG Ruilan, LIU Haoyu, LIU Xiaobin, YU Guanghui. Effects of Different Concentrations of Rutin and Freezing Rates on Cryopreservation of Boar Semen[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 982-991.

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