松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.06.021

摘要/Abstract

摘要： 【目的】分析肝素结合型表皮生长因子样生长因子（heparin-binding EGF-like growth factor，HBEGF）基因单核苷酸多态性（single nucleotide polymorphism，SNP）位点在松辽黑猪中的遗传多样性及其与繁殖性状的相关性。【方法】选择90头健康经产松辽黑猪母猪，采用Sanger直接测序法检测HBEGF基因的SNP位点，利用SPSS 26.0软件分析HBEGF基因SNP位点与松辽黑猪繁殖性状（总产仔数、产活仔数、初生重、3周龄重、断奶重、断奶仔猪数和乳头数）的相关性。【结果】在松辽黑猪HBEGF基因第5外显子及第2、3、4、5内含子上共发现22个SNPs位点，其中有8个SNPs位点与繁殖性状存在显著关联。g.142114384 A>G和g.142104389_142104388ins A>G位点存在AA、AG和GG 3种基因型；g.142114375 C>A位点存在CC、CA和AA 3种基因型；g.142111433 C>T位点存在CC和CT 2种基因型；g.142106003 G>A和g.142105707_142105706ins G>A位点存在GG、GA和AA 3种基因型；g.142105965 C>G位点存在CC、CG和GG 3种基因型；g.142105878_142105877ins C>T位点存在CC、CT和TT 3种基因型。卡方适合性检验结果显示，g.142114375 C>A、g.142111433 C>T和g.142104389_142104388ins A>G位点均处于Hardy-Weinberg平衡状态（P>0.05）。g.142114384 A>G、g.142114375 C>A、g.142106003 G>A、g.142105878_142105877ins C>T和g.142104389_142104388ins A>G位点均为中度多态（0.25<PIC<0.5），g.142111433 C>T、g.142105965 C>G和g.142105707_142105706ins G>A位点均为低度多态（PIC<0.25）。关联分析结果表明，g.142114384 A>G位点AA基因型个体总产仔数、产活仔数、断奶仔猪数均显著高于GG基因型，GG基因型个体初生重显著高于AG基因型，GG基因型个体乳头数显著高于AA、AG基因型（P<0.05）；g.142114375 C>A位点AA基因型个体总产仔数、产活仔数均显著高于CC基因型（P<0.05），AA基因型个体断奶仔猪数显著高于CC、CA基因型（P<0.05）；g.142111433 C>T位点CC基因型个体乳头数显著高于CT基因型（P<0.05）；g.142106003 G>A位点GG基因型个体乳头数显著高于AA基因型（P<0.05）；g.142105965 C>G位点CC、GG基因型个体总产仔数、产活仔数和断奶仔猪数均显著高于CG基因型（P<0.05）；g.142105878_142105877ins C>T位点CC、TT基因型个体断奶仔猪数显著高于CT基因型，TT基因型个体乳头数显著高于CC基因型（P<0.05）；g.142105707_142105706ins G>A位点AA基因型个体3周龄重和断奶重均显著高于GG基因型（P<0.05）；g.142104389_14 2104388ins A>G位点AG基因型个体总产仔数、产活仔数均显著高于AA基因型（P<0.05）。【结论】HBEGF基因中存在8个SNPs位点，与松辽黑猪总产仔数、产活仔数、初生重、3周龄重、断奶重、断奶仔猪数和乳头数均有显著关联。

关键词: 松辽黑猪; HBEGF基因; 繁殖性状; 关联分析; 遗传标记

Abstract: 【Objective】 The genetic diversity of single nucleotide polymorphism (SNP) of heparin-binding EGF-like growth factor (HBEGF) gene in Songliao Black pigs and its correlation with reproductive traits were analyzed.【Method】 The SNP of HBEGF gene were screened by Sanger direct sequencing in 90 healthy Songliao Black sows, and the correlation between SNP of HBEGF gene and reproductive traits (total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglets and nipple number) were analyzed using SPSS 26.0 software.【Result】 A total of 22 SNPs were found in exon 5 and introns 2, 3, 4 and 5 of HBEGF gene in Songliao Black pigs, and a total of 8 SNPs were found to be significantly correlated with reproductive traits.Three genotypes of AA, AG and GG were contained in g.142114384 A>G and g.142104389_142104388ins A>G, three genotypes of CC, CA and AA were contained in g.142114375 C>A, two genotypes of CC and CT were contained in g.142111433 C>T, three genotypes of GG, GA and AA were contained in g.142106003 G>A and g.142105707_142105706ins G>A, three genotypes of CC, CG and GG were contained in g.142105965 C>G, and three genotypes of CC, CT and TT were contained in g.142105878_142105877ins C>T.The chi-square fitness test showed that g.142114375 C>A, g.142111433 C>T and g.142104389_142104388ins A>G were in Hardy-Weinberg equilibrium (P>0.05).g.142114384 A>G, g.142114375 C>A, g.142106003 G>A, g.142105878_142105877ins C>T and g.142104389_142104388ins A>G were moderate polymorphism (0.25<PIC<0.5), g.142111433 C>T, g.142105965 C>G and g.142105707_142105706ins G>A were low polymorphism (PIC<0.25).The results of association analysis showed that the total number born, number born alive and weaning piglets of AA genotype in g.142114384 A>G were significantly higher than those of GG genotype, the birth weight of GG genotype in g.142114384 A>G was significantly higher than that of AG genotype, and the nipple number of GG genotype in g.142114384 A>G was significantly higher than that of AA and AG genotypes (P<0.05).The total number born and number born alive of AA genotype in g.142114375 C>A were significantly higher than those of CC genotype, and the weaning piglets of AA genotype in g.142114375 C>A was significantly higher than that of CC and CA genotypes (P<0.05).The nipple number of CC genotype in g.142111433 C>T was significantly higher than that of CT genotype (P<0.05).The nipple number of GG genotype in g.142106003 G>A was significantly higher than that of AA genotype (P<0.05).The total number born, number born alive and weaning piglets of CC and GG genotypes in g.142105965 C>G were significantly higher than those of CG genotype (P<0.05).The weaning piglets of CC and TT genotypes in g.142105878_1421 05877ins C>T was significantly higher than that of CT genotype, and the nipple number of TT genotype in g.142105878_142105877 ins C>T was significantly higher than that of CC genotype (P<0.05).The 3-week weight and weaning weight of AA genotype in g.142105707_142105706ins G>A were significantly higher than that of GG genotype (P<0.05).The total number born and number born alive of AG genotype in g.142104389_142104388ins A>G was significantly higher than those of AA genotype (P<0.05).【Conclusion】 There were 8 SNPs of HBEGF gene that had significant correlation with total number born, number born alive, birth weight, 3-week weight, weaning weight, weaning piglets and nipple number of Songliao Black pigs.

Key words: Songliao Black pigs; HBEGF gene; reproductive performances; association analysis; genetic markers

中图分类号:

S813.3

张方玮, 张琪, 雷亮亮, 孙武胜, 张迪, 张云鹏, 张净搏, 王修权, 张晶, 张树敏. 松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2023, 50(6): 2370-2379.

ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.

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