鸭胸肌矿物元素含量全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.001

摘要/Abstract

摘要： 【目的】试验旨在利用全基因组关联分析(GWAS)鉴定影响鸭胸肌矿物元素含量的候选基因及分子标记,解析矿物元素性状的遗传机制。【方法】研究构建了北京鸭×野鸭F2代资源群体,利用个体血液样本进行全基因组重测序,采集8周鸭胸肌并根据国标方法测定7种常量矿物元素含量,结合重测序数据与矿物元素表型数据进行全基因关联分析,鉴定影响矿物元素含量相关基因。【结果】7种矿物元素的表型遗传力分别为磷(0.007)＜铁(0.100)＜镁(0.120)＜钠(0.140)＜钾(0.150)＜钙(0.190)＜锌(0.350)。相关性分析结果表明,镁和钾元素相关性最高(r=0.62),磷与镁、钾元素在鸭胸肌中的含量呈现较高的正相关,r值分别为0.43和0.56。通过GWAS鉴定出与锌元素含量显著相关的1个SNP位点在6号染色体12 075 283 bp处达到全基因组水平上的显著关联(-log₁₀P-value=8.03),与最高点SNP高度相关的39个SNPs将候选区间缩小至39 kb,该区间内仅存在1个基因SORCS3,结合基因功能注释与转录组分析确定SORCS3为锌元素含量变异的候选基因。与磷元素含量表型数据在全基因组水平上达到显著相关的最高点SNP与260个SNPs高度相关(R²＞0.4),均位于4号染色体17.1—18.6 Mb之间,且包含27个基因。结合相关性分析、基因功能注释及转录组分析,最终确定6个与磷元素含量高度相关的候选基因:TUSC3、MICU3、LOC101803915、MTMR7、SLC7A2和ASAH1。【结论】本研究利用全基因组关联分析鉴定出鸭胸肌锌元素含量候选基因SORCS3,同时结合转录组分析鉴定出与磷元素含量相关的6个候选基因。结果丰富了禽肉矿物营养的知识体系,为培育优良畜禽产品提供了重要的理论基础。

关键词: 鸭; 胸肌; 矿物元素; 全基因组关联分析; 遗传力

Abstract: 【Objective】 The aim of this study was to identify candidate genes and molecular markers affecting the contents of mineral elements in breast muscle of ducks by genome-wide association study (GWAS),and analyze the genetic mechanism of mineral element traits.【Method】 The F2 generation resource population of Pekin ducks×Anas platyrhynchos was constructed,and the whole genome was resequenced.The breast muscles of ducks were collected for 8 weeks and the contents of 7 major mineral elements were determined according to the national standard method.Combined with the resequencing data and mineral element phenotypic data,the genes related to element content were identified by whole gene association analysis.【Result】 The phenotypic heritability of the contents of seven mineral elements was P(0.007)＜Fe(0.100)＜Mg(0.120)＜Na(0.140)＜K(0.150)＜Ca(0.190)＜Zn(0.350).The results of correlation analysis showed that the correlation between Mg and K was the highest (r=0.62).There was a high positive correlation between P and Mg as well as K in duck breast,r were 0.43 and 0.56,respectively.A SNP locus significantly related to Zn content were identified by GWAS and reached a significant association (-log₁₀P-value=8.03) at 12 075 283 bp on chromosome 6. 39 SNPs highly correlated with the highest point SNP narrowed the candidate interval to 39 kb,in which there was only one gene SORCS3.Combined with gene functional annotation and transcriptome,SORCS3 was identified as a candidate gene for Zn content variation.The highest point of correlation between SNP and P content at the whole genome level was highly correlated with 260 SNPs(R²＞0.4),all of which were located at chromosome 4 between 17.1-18.6 Mb and contained 27 genes.Combined with correlation analysis,gene functional annotation and transcriptome,six candidate genes were highly related to P content were identified (TUSC3,MICU3,LOC101803915,MTMR7,SLC7A2 and ASAH1 genes).【Conclusion】 The candidate gene SORCS3 of Zn content was identified by GWAS,and combined with transcriptome,six candidate genes related to P content were identified.These results enriched the knowledge system of mineral nutrition of poultry meat,and provided an important theoretical basis for breeding excellent livestock and poultry products.

Key words: duck; breast; mineral elements; GWAS; heritability

中图分类号:

S834

余大新, 刘文静, 刘大鹏, 刘同, 张鹤, 盖凯, 李姝楠, 张贝贝, 侯水生, 周正奎. 鸭胸肌矿物元素含量全基因组关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1287-1295.

YU Daxin, LIU Wenjing, LIU Dapeng, LIU Tong, ZHANG He, GAI Kai, LI Shunan, ZHANG Beibei, HOU Shuisheng, ZHOU Zhengkui. Genome-wide Association Studies on Mineral Elements in Breast Muscle of Ducks[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1287-1295.

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