中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (4): 1329-1339.doi: 10.16431/j.cnki.1671-7236.2023.04.005
陈吉敏, 李辉煌, 赖连杰, 杨银华, 杨悦, 林瑞意
发布日期:
2023-04-06
通讯作者:
林瑞意
E-mail:linruiyi@fafu.edu.cn
作者简介:
陈吉敏,E-mail:jmchen@fafu.edu.cn。
基金资助:
CHEN Jimin, LI Huihuang, LAI Lianjie, YANG Yinhua, YANG Yue, LIN Ruiyi
Published:
2023-04-06
摘要: 鸭坦布苏病毒(Duck Tembusu virus,DTMUV)是黄病毒科(Flaviviridae)黄病毒属(Flavivirus)的一种新发蚊媒病毒,其引起的疫病在临床上以蛋鸭产蛋量下降和雏鸭神经系统损伤为特征,给国内养鸭业造成了重大的经济损失。DTMUV感染自2010年在上海市暴发后,逐渐向中国东部、东南部和北部主要鸭养殖场扩散。目前国内已建立了多种用于DTMUV临床诊断或实验室检测方法。DTMUV的分离鉴定和免疫组化用于检测病毒颗粒的存在;DTMUV的血清学检测方法主要包括间接ELISA、竞争ELISA和胶体金免疫层析条等,可检测DTMUV的特异性蛋白或抗体,广泛应用于DTMUV的现场检测;以常规PCR、多重PCR及CRISPR技术为主的病毒核酸检测方法实现了DTMUV的快速临床诊断,促进了鸭坦布苏病毒病的流行病学调查和致病机制研究。随着部分疫苗的开发和商业化,中国DTMUV感染的流行强度也由暴发逐渐转变为零星散发。但DTMUV的宿主范围较广,传播途径多样且具有潜在的人畜共患性,为及早发现并控制DTMUV的传播,建立快速、准确的检测方法和安全有效的疫苗显得尤为重要。笔者对DTMUV的检测方法和疫苗研发的研究进展进行了综述,以期为DTMUV感染的防控提供参考。
中图分类号:
陈吉敏, 李辉煌, 赖连杰, 杨银华, 杨悦, 林瑞意. 鸭坦布苏病毒检测方法及疫苗研究进展[J]. 中国畜牧兽医, 2023, 50(4): 1329-1339.
CHEN Jimin, LI Huihuang, LAI Lianjie, YANG Yinhua, YANG Yue, LIN Ruiyi. Advance on Detection Methods and Vaccines of Duck Tembusu Virus[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1329-1339.
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