绵羊IGF1R基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.05.018

摘要/Abstract

摘要： 【目的】探究胰岛素样生长因子1受体(insulin-like growth factor 1 receptor，IGF1R)基因遗传变异对绵羊生长性状的影响，以期为优质肉羊新品种(系)培育提供有效的分子遗传标记。【方法】利用液相捕获测序技术获得IGF1R基因在杜泊羊、小尾寒羊和滩羊共91只羊中的变异位点，筛选出品种间差异显著的单核苷酸多态性(single nucleotide polymorphism，SNP)位点。针对筛选出的位点利用飞行质谱对3个绵羊品种杂交后代383只绵羊进行检测，并与初生及3月龄绵羊的生长性状进行关联分析。【结果】 3个绵羊品种IGF1R基因共检测到347个突变位点，其中外显子SNPs共8个:g.7628315 T>C、g.7628528 T>C、g.7628690 C>T、g.7833817 C>T、g.7836687 C>T、g.7840691 T>C、g.7855904 C>G、g.7872277 C>T，在群体中均表现出多态性，χ²检验结果表明，除小尾寒羊g.7628690 C>T位点外其余位点均处于Hardy-Weinberg平衡状态。g.7628528 T>C和g.7872277 C>T位点在3个绵羊群体中均表现为低度多态(PIC<0.25);g.7833817 C>T位点在3个绵羊群体中均表现为中度多态(0.25<PIC<0.50);其余5个位点表现为在1或2个群体内的中度多态。单个位点关联分析表明，g.7628528 T>C位点与F2代的3月龄体重、胸围，H2代的初生胸围均呈显著相关(P<0.05);g.7628690 C>T位点与H2代的初生胸围呈显著相关(P<0.05);g.7833817 C>T位点与F2代的3月龄体高呈显著相关(P<0.05);g.7836687 C>T位点与F1代的3月龄体重和胸围、F2代的3月龄体高均呈显著相关(P<0.05);g.7855904 C>G位点与F1代的3月龄胸围、F2代的初生胸围均呈显著相关(P<0.05);g.7872277 C>T位点与H1代的3月龄胸围，H2代的初生体重、体高、胸围及3月龄体高均呈显著关联(P<0.05);g.7628315 T>C位点与F1代的3月龄体高、体斜长，H2代的3月龄体高均呈显著相关(P<0.05);g.7840691 T>C位点与生长性状无显著关联(P>0.05)。连锁不平衡分析发现，g.7628315 T>C-g.7628528 T>C和g.7833817 C>T-g.7840691 T>C形成了2处强连锁，均构建出3种单倍型，组合后均形成6种基因型，其中优势基因型分别为H2H2和H5H6。单倍型组合关联分析发现，H3H1基因型个体初生体斜长显著高于H3H2基因型(P<0.05);H2H2基因型个体3月龄体高显著高于H1H2基因型(P<0.05);H4H5基因型个体3月龄体斜长显著高于H4H6基因型(P<0.05);其他生长指标在各组合基因型间差异均不显著(P>0.05)。【结论】 IGF1R基因的遗传变异对绵羊的生长性状有显著影响，8个SNPs位点可作为绵羊生长发育过程中的潜在候选遗传标记，本研究结果可为IGF1R基因在肉羊中的选种选育提供重要参考，为肉羊的分子标记辅助育种提供理论依据。

关键词: 绵羊; IGF1R基因; 生长性状; 关联分析

Abstract: 【Objective】 This study was aimed to explore the influence of genetic variation of insulin-like growth factor 1 receptor (IGF1R) gene on the growth traits of sheep (Ovis aries),in order to provide effective molecular genetic markers for the breeding of new breeds (lines) of high-quality mutton sheep.【Method】 Using liquid-phase capture sequencing technology to obtain IGF1R gene mutation sites in a total of 91 Doper sheep,Small-tailed Han sheep and Tan sheep,and screen out single nucleotide polymorphism (SNP) with significant differences among breeds.For the selected sites,flight mass spectrometry was used to detect 383 sheep of the hybrid offspring of 3 sheep breeds,and the correlation analysis with growth traits of newborn and 3 months old was carried out.【Result】 A total of 347 mutation sites were detected in IGF1R gene of 3 sheep breeds,there were 8 SNPs in exon:g.7628315 T>C,g.7628528 T>C,g.7628690 C>T,g.7833817 C>T,g.7836687 C>T,g.7840691 T>C,g.7855904 C>G and g.7872277 C>T.which showed polymorphism in the population.χ² test results showed that all SNPs were in Hardy-Weinberg equilibrium (expect for g.7628690 C>T in Small-tail Han sheep).g.7628528 T>C and g.7872277 C>T showed low polymorphism in 3 sheep breeds (PIC<0.25);g.7833817 C>T showed moderate polymorphism in 3 sheep breeds (0.25<PIC<0.50);The other 5 SNPs showed moderate polymorphism in 1 or 2 populations.The single-site association analysis showed that g.7628528 T>C was significantly correlated with the 3 months old body weight and chest girth of F2 generation,and the newborn chest girth of H2 generation (P<0.05);g.7628690 C>T was significantly correlated with the newborn chest girth of H2 generation (P<0.05);g.7833817 C>T was significantly correlated with the 3 months old body height of F2 generation (P<0.05);g.7836687 C>T was significantly correlated with the 3 months old body weight and chest girth of F1 generation,and the 3 months old body height of F2 generation (P<0.05);g.7755904 C>G was significantly correlated with the 3 months old chest girth of F1 generation,and the newborn chest girth of F2 generation (P<0.05);g.7872277 C>T was significantly correlated with the 3 months old chest girth of H1 generation,the newborn body weight,body height,chest girth,and 3 months old body height of H2 generation (P<0.05);g.7628315 T>C was significantly correlated with the 3 months old body height and body length of F1 generation,and the 3 months old body height of H2 generation (P<0.05);g.7840691 T>C had no significant association with growth traits (P>0.05).Linkage disequilibrium analysis showed that g.7628315 T>C-g.7628528 T>C and g.7833817 C>T-g.7840691 T>C formed 2 strong linkages,3 haplotypes were constructed,and 6 genotypes were formed after combination,the dominant genotypes were H2H2 and H5H6,respectively.The association analysis of haplotype combination showed that the newborn body length of H3H1 genotype individuals was significantly higher than that of H3H2 genotype (P<0.05),and the 3 months old body height of H2H2 genotype individuals was significantly higher than that of H1H2 genotype (P<0.05),the 3 months old body length of H4H5 genotype individuals was significantly higher than that of H4H6 genotype (P<0.05),and there were no significant differences in other growth indicators among the combined genotypes (P>0.05).【Conclusion】 The results of this study indicated that the genetic variation of IGF1R gene had an significant impact on the growth traits of sheep.8 SNPs caould be considered as potential candidate genetic markers during the growth and development of sheep,which could provide information for the selection and breeding of IGF1R gene in mutton sheep,and provide the theoretical basis for the molecular marker-assisted breeding of mutton sheep.

Key words: sheep; IGF1R gene; growth traits; association analysis

中图分类号:

S813.3

强浩, 梁鹏, 孟科, 荣轩, 冯登侦. 绵羊IGF1R基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2022, 49(5): 1765-1785.

QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.

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