白羽王鸽MyoG基因多态性及其与体尺和肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.018

摘要/Abstract

摘要： 【目的】研究白羽王鸽肌细胞生成素(myogenin,MyoG)基因多态性及其与体尺和肉质性状的关系,为该品种的分子标记选育工作提供参考依据。【方法】以108只12周龄的白羽王鸽为研究对象,利用PCR直接测序法筛查白羽王鸽MyoG基因单核苷酸多态性(single nucleotide polymorphism,SNP)位点,通过RNAfold预测MyoG基因突变前后的mRNA二级结构,并利用SPSS 26.0软件分析MyoG基因SNP位点与白羽王鸽体尺和肉质性状的相关性。【结果】在白羽王鸽MyoG基因外显子3上检测到4个SNPs位点:g.590075 C＞T、g.590084 G＞A、g.590102 G＞A和g.590114 C＞T,均为同义突变,且均产生3种基因型,其中,g.590075 C＞T位点呈低度多态,其余3个SNPs位点均呈中度多态。卡方检验结果显示,g.590084 G＞A和g.590114 C＞T位点均符合Hardy-Weinberg平衡(P＞0.05),且两个位点之间存在强连锁不平衡。g.590084 G＞A和g.590102 G＞A位点突变导致MyoG基因mRNA序列二级结构和自由能发生改变。4个SNPs联合产生4种单倍型和9种双倍型。关联分析结果表明,g.590075 C＞T位点与白羽王鸽龙骨长呈极显著关联(P＜0.01);g.590102 G＞A位点对体斜长有极显著效应(P＜0.01);双倍型H3H3(CCAAAACC)个体的体斜长优于其余双倍型,H4H4(TTGGGGCC)个体在龙骨长和胸宽方面优于其他双倍型。g.590084 G＞A和g.590114 C＞T位点与白羽王鸽胸肌的剪切力呈极显著或显著关联(P＜0.01;P＜0.05);双倍型H3H3(CCAAAACC)个体在胸肌的pH和肉色方面优于其余双倍型,而在剪切力和失水率方面,双倍型H2H4(CTGAGGCT)和H4H4(TTGGGGCC)表现较优。【结论】MyoG基因对白羽王鸽部分体尺和肉质性状有显著影响,可作为白羽王鸽新品系培育的候选基因。

关键词: 白羽王鸽; MyoG基因; 多态性; 体尺性状; 肉质性状; 关联分析

Abstract: 【Objective】 The purpose of this experiment was to study the polymorphism of myogenin (MyoG) gene and its relationship with body size and meat quality traits in White feather King pigeons,and provide reference for the breeding of molecular markers in this breed.【Method】 A total of 108 12-week-old White feather King pigeons were selected as the research objects,PCR and direct sequencing were used to screen the SNP of MyoG gene in White feather King pigeons,RNAfold was used to predict the mRNA secondary structure before and after MyoG gene mutation,and SPSS 26.0 software was used to analyze the correlation between SNP of MyoG gene and body size and meat quality traits of White feather King pigeons.【Result】 Four SNPs were detected in exon 3 of MyoG gene in White feather King pigeons:g.590075 C＞T,g.590084 G＞A,g.590102 G＞A and g.590114 C＞T,all of which were synonymous mutations and resulted in three genotypes,among them,g.590075 C＞T belonged to low polymorphism,the other 3 SNPs showed moderate polymorphism.χ² results showed that g.590084 G＞A and g.590114 C＞T were in Hardy-Weinberg equilibrium (P＞0.05),and there was strong linkage disequilibrium between the two SNPs.The mutations of g.590084 G＞A and g.590102 G＞A resulted in the change of secondary structure and free energy of MyoG gene mRNA sequence.The combination of 4 SNPs produced 4 haplotypes and 9 diplotypes.The results of correlation analysis showed that g.590075 C＞T was extremely significantly associated with keel length in White feather King pigeons (P＜0.01);g.590102 G＞A had an extremely significant effect on body length (P＜0.01).The body length of H3H3 (CCAAAACC) individuals were superior to the other diplotypes,and the keel length and chest width of H4H4 (TTGGGGCC) individuals were superior to the other diplotypes.g.590084 G＞A and g.590114 C＞T were extremely significantly or significantly associated with pectoral muscle shear force (P＜0.01 or P＜0.05).The diplotype H3H3 (CCAAAACC) individuals were better than the other diplotypes in pH and flesh color of chest muscle,while in the shear force and water loss rate,the diplotype H2H4 (CTGAGGCT) and H4H4 (TTGGGGCC) performed better.【Conclusion】 MyoG gene had significant effect on some body size and meat quality traits in White feather King pigeons,which could be used as a candidate gene for breeding new lines in White feather King pigeons.

Key words: White feather King pigeons; MyoG gene; polymorphism; body size trait; meat quality trait; association analysis

中图分类号:

S813.3

谭元成, 罗华伦, 张依裕, 杨酸, 王盈童, 王春源. 白羽王鸽MyoG基因多态性及其与体尺和肉质性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1461-1471.

TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.

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