黔东南小香鸡MEF2C基因外显子多态性及其与体重、体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.07.019

摘要/Abstract

摘要： 【目的】探究肌细胞增强因子2C（myocyte enhancer factor 2C，MEF2C）基因多态性对黔东南小香鸡体重和体尺性状的影响。【方法】试验以251羽300日龄黔东南小香鸡为研究对象，利用PCR扩增和直接测序技术对MEF2C基因全部（15个）外显子进行单核苷酸多态性（single nucleotide polymorphism，SNP）位点筛选，并运用SPSS 18.0统计分析软件对不同SNPs位点所对应的基因型与黔东南小香鸡的体重、体尺性状进行相关性分析。【结果】黔东南小香鸡群体中仅在MEF2C基因外显子12上发现3个SNPs位点：g.1819 T＞C、g.2426 T＞C和g.2543 C＞T，在其他外显子中未发现多态位点。χ²检验结果表明，3个SNPs在黔东南小香鸡群体中均偏离Hardy-Weinberg平衡状态（P＞0.05）。关联分析结果显示，g.1819 T＞C位点TC基因型个体体斜长、胸深和胫围均极显著高于TT和CC基因型，体重极显著高于TT基因型（P＜0.01）；TC基因型个体胫长显著高于TT和CC基因，龙骨长显著高于TT基因型（P＜0.05）。g.2426 T＞C和g.2543 C＞T位点互为连锁平衡位点，对胫围有极显著影响（P＜0.01）。【结论】黔东南小香鸡MEF2C基因外显子具有较强的保守性，g.1819 T>C位点可以作为黔东南小香鸡分子标记辅助选育参考位点。

关键词: 黔东南小香鸡; MEF2C基因; 多态性; 体重; 体尺; 关联分析

Abstract: 【Objective】 This study was aimed to investigate the effect of myocyte enhancer factor 2C (MEF2C) gene polymorphism on the body weight and body size traits in Qiandongnan Xiaoxiang chickens.【Method】 251 300-day-old Qiandongnan Xiaoxiang chickens were used as the research object,the polymorphism of all (15) exons of MEF2C gene were analyzed by PCR amplification and direct sequencing technology,and the single nucleotide polymorphism (SNP) was screened.The correlation between the genotypes corresponding to different SNPs and body weight or body size traits in Qiandongnan Xiaoxiang chickens were analyzed using SPSS 18.0 statistical analysis software.【Result】 Only 3 SNPs (g.1819 T＞C,g.2426 T＞C and g.2543 C＞T) were found in exon 12 of MEF2C gene in Qiandongnan Xiaoxiang chickens,and no SNP was found in other exons.χ² test results showed that 3 SNPs deviated from Hardy-Weinberg equilibrium in Qiandongnan Xiaoxiang chickens (P＞0.05).The results of association analysis showed that the body oblique length,chest depth,and tibia circumference of g.1819 T＞C TC genotype were extremely significantly higher than those of TT and CC genotypes,and the body weight was extremely significantly higher than that of TT genotype (P＜0.01);The tibial length of TC genotype was significantly higher than that of TT and CC genotypes,and the keel length was significantly higher than that of TT genotype (P＜0.05).g.2426 T＞C and g.2543 C＞T were linkage balance sites,which had extremely significant effect on the tibia circumference (P＜0.01).【Conclusion】 The exon of MEF2C gene in Qiandongnan Xiaoxiang chickens were highly conserved,and g.1819 T＞C could be used as a reference site for molecular marker-assisted breeding of Qiandongnan Xiaoxiang chickens.

Key words: Qiandongnan Xiaoxiang chickens; MEF2C gene; polymorphism; body weight; body size; association analysis

中图分类号:

S831.2

周迪, 敖叶, 蒋桂荣, 赵忠海, 李俊, 杨蓉, 龚菲, 陈昌雪, 沈银, 李辉. 黔东南小香鸡MEF2C基因外显子多态性及其与体重、体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(7): 2613-2621.

ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.

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