黔北麻羊MC4R基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.01.020

摘要/Abstract

摘要： 【目的】探究黔北麻羊黑素皮质素受体-4（melanocortin receptor-4，MC4R）基因多态性及其与生长性状的关联性，为黔北麻羊的选种选育提供理论依据。【方法】选取196只6月龄黔北麻羊，利用DNA混合池结合Sanger测序筛选MC4R基因单核苷酸多态性（SNP）位点，并采用SPSS 22.0软件中的一般线性模型（GLM）对MC4R基因SNP位点与黔北麻羊生长性状进行关联分析。【结果】在黔北麻羊MC4R基因中发现4个SNPs位点：g.59345469 C>A和g.59346773 T>C位点分别位于5'-和3'-端非编码区（UTR）；g.59345871 A>C位点突变导致第37位天冬氨酸（Asp）变为丙氨酸（Ala），引起RNA二级结构及蛋白质二级结构、三级结构发生明显改变；g.59346263 A>G位点突变导致第168位异亮氨酸（Ile）变为缬氨酸（Val），对RNA二级结构和蛋白质二级结构有明显影响。关联分析结果显示，g.59345469 C>A位点对黔北麻羊体重、胸围和管围均有显著影响（P<0.05）；g.59345871 A>C位点对黔北麻羊体重、体高、管围和胸围均有显著影响（P<0.05）；g.59346263 A>G位点对黔北麻羊体高、体重和胸围均有显著影响（P<0.05）；g.59346773 T>C位点对黔北麻羊体重和胸围均有显著影响（P<0.05）。4个SNPs位点联合共检测到9种单倍型和21种双倍型，其联合对黔北麻羊体重、体高、胸围和管围均产生显著遗传效应（P<0.05），纯合双倍型H5H5（AAAAGGCC）个体的生长性状优于其他双倍型。【结论】黔北麻羊MC4R基因存在4个SNPs位点，与黔北麻羊体重和胸围均存在显著关联，可作为黔北麻羊体重和胸围的遗传标记用于分子育种。

关键词: 黔北麻羊; MC4R基因; 单核苷酸多态性(SNP); 生长性状

Abstract: 【Objective】 The aim of this study was to explore melanocortin receptor-4 (MC4R) gene polymorphism and its correlation with growth traits in Qianbei Ma goats, in order to provide theoretical basis for breed selection and breeding of Qianbei Ma goats.【Method】 196 six-month-old Qianbei Ma goats were used as experimental materials, the single nucleotide polymorphism (SNP) of MC4R gene was screened by DNA mixing pool combined with Sanger sequencing, and the general linear model (GLM) in SPSS 22.0 software was used to analyze the correlation between SNP of MC4R gene and growth traits of Qianbei Ma goats.【Result】 There were 4 SNPs of MC4R gene in Qianbei Ma goats, g.59345469 C>A and g.59346773 T>C were located in the 5'- and 3'-untranslated region (UTR), respectively.The mutation of g.59345871 A>C resulted in the transformation of aspartic acid (Asp) to alanine (Ala) at position 37, and caused significant changes in the secondary structure of RNA and the secondary and tertiary structures of protein.The mutation of g.59346263 A>G resulted in the transformation of isoleucine (Ile) to valine (Val) at position 168, which had significant effects on the secondary structure of RNA and the secondary structure of protein.The results of association analysis showed that g.59345469 C>A had significant effect on the body weight, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05). g.59345871 A>C had significant effect on the body weight, body height, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05).g.59346263 A>G had significant effects on the body height, body weight and chest circumference in Qianbei Ma goats (P<0.05).g.59346773 T>C had significant effect on the body weight and chest circumference in Qianbei Ma goats (P<0.05).A total of 9 haplotypes and 21 diplotypes were detected by the combination of 4 SNPs, the combination had significant genetic effects on the body weight, body height, chest circumference and cannon circumference in Qianbei Ma goats (P<0.05), the growth traits of homozygous diplotype H5H5 (AAAAGGCC) individual was superior to that of other diplotypes.【Conclusion】 Four SNPs of MC4R gene were significantly associated with the body weight and chest circumference in Qianbei Ma goats, which could be used as genetic markers for molecular breeding of Qianbei Ma goats.

Key words: Qianbei Ma goats; MC4R gene; single nucleotide polymorphism (SNP); growth traits

中图分类号:

S813.3

龙安炬, 杨酸, 艾锦新, 翁吉梅, 张依裕, 蔡惠芬, 罗卫星. 黔北麻羊MC4R基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2023, 50(1): 198-209.

LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.

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