绵羊MyoG基因内含子Ⅱ多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.04.018

摘要/Abstract

摘要： 【目的】分析肌细胞生成素(myogenin,MyoG)基因内含子Ⅱ的多态性及其对绵羊生长性状的影响,筛选对绵羊生长发育有显著影响的分子标记,以期为绵羊的分子育种提供参考。【方法】选取大尾寒羊、小尾寒羊、豫西脂尾羊、湖羊、杜泊羊5种绵羊为研究对象,采用PCR-RFLP技术对MyoG基因内含子Ⅱ(Eco72 Ⅰ)进行基因分型,利用PopGene32软件计算绵羊MyoG基因内含子Ⅱ的群体遗传多样性,利用SPSS 17.0软件对不同基因型与绵羊生长性状进行关联分析。【结果】小尾寒羊、杜泊羊、湖羊群体中MyoG基因内含子Ⅱ均存在3种基因型:AA(368/540 bp)、AB(908/368/540 bp)和BB(908 bp);大尾寒羊和豫西脂尾羊群体中仅检测到2种基因型:AB(908/368/540 bp)和BB(908 bp)。大尾寒羊、小尾寒羊、豫西脂尾羊、湖羊、杜泊羊的AB基因型频率分别为0.845、0.633、0.917、0.706和0.811,BB基因型频率分别为0.155、0.033、0.083、0.176和0.054,AA基因型频率分别为0、0.333、0、0.118和0.135。小尾寒羊的杂合度最低(0.455),杜泊羊的杂合度最高(0.497),表明杜泊羊的遗传多样性要高于其他群体;5个群体多态信息含量(PIC)均为中度多态(0.25<PIC<0.5)。关联分析发现,小尾寒羊MyoG基因内含子Ⅱ BB基因型个体胸围、胸宽、头长、颈长均显著低于AA、AB基因型(P<0.05)。【结论】 MyoG基因内含子Ⅱ Eco72 Ⅰ位点可作为影响绵羊生长性状的分子标记,结果可为今后绵羊生长性状的分子标记辅助选择提供参考。

关键词: 绵羊; MyoG基因; 内含子Ⅱ; Eco72 Ⅰ位点; 生长性状

Abstract: 【Objective】 This study was aimed to analyze the polymorphism of myogenin (MyoG) gene intron Ⅱ and its effect on growth traits in sheep,the molecular markers with significant effects on the growth and development of sheep were screened to provide reference for molecular breeding in sheep.【Method】 Five species of Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Dorper sheep and Hu sheep were selected as the research object. The intron Ⅱ of MyoG gene (Eco72 Ⅰ) was genotyped by PCR-RFLP technology.The population genetic polymorphism of MyoG gene intron Ⅱ was calculated by PopGene32 software.The association analysis between the different genotypes of MyoG gene intron Ⅱ and growth traits of sheep was performed by SPSS 17.0 software.【Result】 There were three genotypes of AA (368/540 bp),AB (908/368/540 bp) and BB (908 bp) were found of MyoG gene intron Ⅱ in Small-tailed Han sheep,Dorper sheep and Hu sheep populations,and there were two genotypes of AB (908/368/540 bp) and BB (908 bp) were found in Large-tailed Han sheep and Yuxi Fat-tailed sheep populations.The genotype frequency of AB in Large-tailed Han sheep,Small-tailed Han sheep,Yuxi Fat-tailed sheep,Hu sheep and Dorper sheep were 0.845,0.633,0.917,0.706 and 0.811,the genotype frequency of BB were 0.155,0.033,0.083,0.176 and 0.054,and the genotype frequency of AA genotype were 0,0.333,0,0.118 and 0.135,respectively.The lowest heterozygosity was 0.455 in Small-tailed Han sheep,and the highest was 0.497 in Dorper sheep,indicating that Dorper sheep had a higher genetic diversity than other population.The polymorphic information content (PIC) of five sheep population was moderate polymorphism (0.25<PIC<0.5).The association analysis results showed that the chest circumference,chest width,head length and neck length of BB genotype of MyoG gene intron Ⅱ in Small-tailed Han sheep were significantly lower than those of AA and AB genotypes (P<0.05).【Conclusion】 The Eco72 Ⅰ site of MyoG gene intron Ⅱ could be used as a molecular marker affecting the growth trait in sheep,and the results provided reference for molecular marker assisted selection of growth trait in sheep in the future.

Key words: sheep; MyoG gene; intron Ⅱ; Eco72 Ⅰ site; growth traits

中图分类号:

S813.3

李静云, 王新乐, 张云飞, 白俊艳, 李淦, 雷莹, 董智豪, 陈宇, 樊红灯, 王龙威, 陈梦柯, 王兴科, 司马磊阳, 张世文. 绵羊MyoG基因内含子Ⅱ多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2022, 49(4): 1364-1373.

LI Jingyun, WANG Xinle, ZHANG Yunfei, BAI Junyan, LI Gan, LEI Ying, DONG Zhihao, CHEN Yu, FAN Hongdeng, WANG Longwei, CHEN Mengke, WANG Xingke, SIMA Leiyang, ZHANG Shiwen. Polymorphism of MyoG Gene Intron Ⅱ and Its Association with Growth Traits in Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1364-1373.

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