野生盘羊×巴什拜羊杂交二代尾部脂肪差异表达lncRNA的鉴定与分析

doi:10.16431/j.cnki.1671-7236.2022.05.020

摘要/Abstract

摘要： 【目的】分析不同脂尾型绵羊尾部脂肪组织中的长链非编码RNA(long non-coding RNA，lncRNA)的表达谱，探究lncRNA与绵羊尾部脂肪沉积机制的关系，为揭示绵羊尾脂沉积机制提供理论依据。【方法】选择新疆地方绵羊巴什拜羊(肥尾型)和野生盘羊×巴什拜羊杂交二代(小尾型)作为研究对象，采集2个群体尾部脂肪组织，利用转录组测序技术筛选差异表达lncRNA并预测靶基因，对其进行GO功能和KEGG通路富集分析;利用实时荧光定量PCR技术验证转录组测序的表达结果。【结果】通过差异表达分析共筛选出728个差异表达lncRNAs，其中270个表达下调，458个表达上调;通过差异表达lncRNA靶基因的GO功能和KEGG通路富集分析，筛选到634个靶基因参与疾病、免疫、蛋白修饰、细胞代谢等相关功能分类，共涉及76条信号通路，部分lncRNA介导的靶基因SCD、GPAM、THRSP、FASN等与绵羊尾部脂肪沉积相关。实时荧光定量PCR与转录组测序结果趋势相同。【结论】 lncRNA在绵羊脂尾进化中对尾部脂肪沉积具有重要的调控作用，为从lncRNA的角度分析绵羊脂肪沉积提供了理论依据。

关键词: 绵羊; 尾部脂肪; 脂肪沉积; lncRNA; 转录组测序

Abstract: 【Objective】 The purpose of this study was to analyze the expression profile of long non-coding RNA (lncRNA) in tail fat tissues of different tail fat-type sheep,and explore the relationship between lncRNA and tail fat deposition mechanism in sheep,so as to provide a theoretical basis for revealing the mechanism of tail fat deposition in sheep.【Method】 Xinjiang local sheep Bashibai sheep(fat tail-type) and the hybrid second generation of wild Argali×Bashibai sheep (small tail-type) were selected as the research objects.The tail fat tissues of two populations were collected,the transcriptome sequencing technology was used to screen the differentially expressed lncRNA and predict target gene,and GO function and KEGG pathway enrichment were analyzed.The expression results of transcriptome sequencing were verified by Real-time quantitative PCR.【Result】 728 differentially expressed lncRNAs were screened by differential expression analysis,of which 270 lncRNAs were down-regulated,and the expression of 458 lncRNAs was up-regulated.The target gene was screened by GO function and KEGG pathway enrichment analysis of differentially expressed lncRNA,there were 634 target genes involved in diseases,immunity,protein modification,cell metabolism and other related functions,involving a total of 76 signal pathways.Some lncRNA-mediated target genes SCD,GPAM,THRSP,FASN,etc,were related to tail fat deposition in sheep.The result of Real-time quantitative PCR and transcriptome sequencing was consistent.【Conclusion】 lncRNA played an important role in regulating tail fat deposition in the evolution of tail fat in sheep,which provided theoretical basis for analyzing fat deposition in sheep from the perspective of lncRNA.

Key words: sheep; tail fat; fat deposition; lncRNA; RNA-Seq

中图分类号:

S826

苏晓慧, 何海迎, 吐尔逊阿依·牙力坤, 王琼, 马海玉, 刘玲玲, 刘武军. 野生盘羊×巴什拜羊杂交二代尾部脂肪差异表达lncRNA的鉴定与分析[J]. 中国畜牧兽医, 2022, 49(5): 1796-1805.

SU Xiaohui, HE Haiying, TUERXUNAYI·Yalikun, WANG Qiong, MA Haiyu, LIU Lingling, LIU Wujun. Identification and Analysis of Differentially Expressed lncRNA of Tail Fat in the Second Generation of Crossbred from Wild Argali×Bashibai Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1796-1805.

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