关岭牛TBC1D7基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.08.018

摘要/Abstract

摘要： 为探究TBC1蛋白家族第7成员（TBC1 domain family member 7，TBC1D7）基因单核苷酸多态性（SNP）对关岭牛生长性状的影响，试验选取116头贵州关岭牛，测定其8项生长性状指标，提取血液基因组DNA，并利用DNA混池扩增TBC1D7基因全部外显子，通过直接测序法筛查SNP。利用不同独立样本DNA扩增SNP位点对应外显子并测序，以验证位点并进行基因分型。利用SPSS 19.0软件单因素方差分析TBC1D7基因不同基因型和关岭牛生长性状之间的相关性。结果显示，在TBC1D7基因第5、6和8外显子上共发现5个SNPs位点：g.12972 T>C、g.12984 A>G、g.20538 C>T、g.20577 T>C和g.24807 G>A，均存在3种基因型；χ²检验结果显示，5个SNPs位点均显著偏离Hardy-Weinberg平衡状态（P<0.05）；群体遗传参数分析发现，5个SNPs位点杂合度较高，多态信息含量均表现为中度多态（0.25<PIC<0.5）。关联性分析结果发现，关岭牛TBC1D7基因g.24807 G>A位点GA基因型个体体斜长、胸围和后臀围均显著高于AA基因型个体（P<0.05）。在本试验群体中，5个SNPs存在6种单倍型（H1~H6）和9种双倍型（H1H1、H2H2、H2H3、H2H6、H3H3、H3H4、H3H5、H3H6和H5H5），其中H3和H2为优势单倍型，H3H3、H2H2和H2H3为优势双倍型；双倍型关联分析结果显示，H3H6和H5H5个体体斜长和后臀围相比其他双倍型个体占有明显优势，可能是有利基因型。结果表明，TBC1D7基因g.24807 G>A位点为影响关岭牛生长发育的重要多态位点，本研究为筛选有助于关岭牛生长发育的分子标记提供了理论参考。

关键词: 关岭牛; TBC1D7基因; SNPs; 生长性状; 相关性

Abstract: In order to investigate the effect of single nucleotide polymorphism (SNP) of TBC1 domain family member 7(TBC1D7) gene on growth traits in Guanling cattle, in this study, blood samples were collected from 116 Guizhou Guanling cattle, and 8 growth traits were determined.All exons of TBC1D gene were amplified by mixed DNA pool, SNP was screened by direct sequencing, and then amplified and sequenced with individual sample DNA as template to verify SNP and used for genotyping.The correlation between different genotypes of TBC1D gene and the growth traits of Guanling cattle was analyzed by single factor variance of SPSS 19.0 software.The results showed that g.12972 T>C, g.12984 A>G, g.20538 C>T, g.20577 T>C and g.24807 G>A mutation sites were found in exons 5, 6 and 8 of TBC1D gene.The results of genotyping showed that there were 3 genotypes in all 5 SNPs.Chi-square (χ²) test showed that 5 SNPs were significantly deviated from Hardy-Weinberg equilibrium state (P<0.05).The analysis of population genetic parameters showed that 5 SNPs had high heterozygosity and moderately polymorphic information content (0.25<PIC<0.5).The correlation analysis results showed that the oblique length, chest circumference and rear hip circumference of GA genotype individuals in g.24807 G>A of TBC1D gene were significantly higher than that of AA genotype individuals (P<0.05).There were 6 haplotypes(H1-H6) and 9 diplotypes(H1H1, H2H2, H2H3, H2H6, H3H3, H3H4, H3H5, H3H6 and H5H5) of 5 SNPs in the experimental population, including dominant haplotypes H3 and H2, dominant diplotypes H3H3, H2H2 and H2H3.The results of diplotype association analysis showed that H3H6 and H5H5 individuals had significant advantages in body length and hip circumference compared with other diplotypes, and H3H6 and H5H5 might be the favorable genotypes.This results showed that g.24807 G>A of TBC1D gene was an important SNP affecting the growth and development of Guanling cattle, which provided a theoretical reference for screening molecular markers that were helpful to the growth and development of Guanling cattle.

Key words: Guanling cattle; TBC1D7 gene; SNPs; growth trait; correlation

中图分类号:

S813.3

刘鹏程, 张继, 邱淦远, 龚俞, 李雪松, 李维, 张依裕, 刘若余. 关岭牛TBC1D7基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(8): 2853-2863.

LIU Pengcheng, ZHANG Ji, QIU Ganyuan, GONG Yu, LI Xuesong, LI Wei, ZHANG Yiyu, LIU Ruoyu. Polymorphism of Bovine TBC1D Gene and Its Association with Growth Traits in Guanling Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2853-2863.

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