梅花鹿MSRB3基因多态性及其与茸重性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.11.018

摘要/Abstract

摘要： 【目的】本研究旨在探索梅花鹿甲硫氨酸亚砜还原酶B3（methionine sulfoxide reductase B3，MSRB3）基因多态性及其与茸重性状的关联性。【方法】选取高、低产茸量的梅花鹿各8只，应用DNA直接测序法检测基因变异位点。采用MassARRAY^® SNP分型技术对314头24月龄梅花鹿MSRB3基因进行基因分型，并结合梅花鹿茸重性状数据进行了关联分析和单倍型分析。【结果】在梅花鹿MSRB3基因中共发现6个SNPs位点，其中2个SNPs位点位于外显子区域，且突变均未引起氨基酸改变，属于同义突变，其余4个SNPs位点均存在于内含子区域。分型结果显示4个样本未分型成功，其余310个样本进行后续分析。各位点观测杂合度和期望杂合度基本一致，g.44455582 T>C、g.44455759 C>T、g.44414424 T>C、g.44350306 T>C及g.44340836 G>A等5个位点的杂合度较高，均属于中度多态（0.25<PIC<0.5），g.44340734 G>C位点杂合度较低，属于低度多态（PIC<0.25）。卡方检验结果表明，g.44455759 C>T位点偏离Hardy-Weinberg平衡状态（P<0.05），其他5个位点均处于Hardy-Weinberg平衡状态（P>0.05）。对6个SNPs位点的不同基因型与梅花鹿茸重的关联分析结果表明，g.44455582 T>C位点的TT基因型个体茸重极显著高于CC和TC基因型（P<0.01）。单倍型分析结果显示，g.44340734 G>C和g.44350306 T>C、g.44455582 T>C和g.44455759 C>T位点存在强连锁，各产生3种单倍型，在Block 2区块中单倍型TC茸重显著高于单倍型CT （P<0.05）。【结论】 MSRB3基因与梅花鹿茸重性状密切相关，g.44455582 T>C位点和单倍型TC可作为筛选高产梅花鹿的分子标记。

关键词: 梅花鹿; MSRB3基因; SNPs; 茸重

Abstract: 【Objective】 This study aimed to explore the polymorphism of methionine sulfoxide reductase B3 (MSRB3) gene and its association with antler weight trait.【Method】 8 sika deer with high and 8 sika deer with low antler yield were selected, and the gene mutation sites were detected by direct DNA sequencing. The genetic variation of MSRB3 gene in 314 24-month-old sika deer was genotyped by MassARRAY^® SNP typing technology, and the association analysis and haplotype analysis were carried out combined with the data of antler weight traits of sika deer.【Result】 A total of 6 SNPs were found in MSRB3 gene of sika deer, of which 2 SNPs were located in the exon region, and the mutations did not cause amino acid changes, belonging to synonymous mutations, and the other 4 SNPs were located in the intron region.The typing results showed that 4 samples were not successfully typed, and the remaining 310 samples were subjected to follow-up analysis.The observed heterozygosity of each locus was basically consistent with the expected heterozygosity.The heterozygosity of g.44455582 T>C, g.44455759 C>T, g.44414424 T>C, g.44350306 T>C and g.44340836 G>A were higher, which showed moderately polymorphic (0.25<PIC<0.5).And g.44340734 G>C locus had low heterozygosity, which showed low polymorphism (PIC<0.25).The results of chi-square test showed that g.44455759 C>T deviated from Hardy-Weinberg equilibrium (P<0.05), and the other 5 loci were in Hardy-Weinberg equilibrium (P>0.05).The results of association analysis between the different genotypes of 6 SNPs and antler weight of sika deer showed that the antler weight of TT genotype with g.44455582 T>C locus was extremely significantly higher than that of CC and TC genotypes (P<0.01).The haplotype analysis results showed that g.44340734 G>C and g.44350306 T>C, g.44455582 T>C and g.44455759 C>T were strongly linked, each producing 3 haplotypes.The antler weight of haplotype TC in Block 2 was significantly higher than that of haplotype CT (P<0.05).【Conclusion】 MSRB3 gene was closely related to the antler weight traits of sika deer, g.44455582 T>C locus and haplotype TC could be used as molecular markers for screening high-yield sika deer.

Key words: sika deer; MSRB3 gene; SNPs; antler weight

中图分类号:

S825

周雅, 张禾垟, 刘琳玲, 李浩东, 郑军军, 王桂武. 梅花鹿MSRB3基因多态性及其与茸重性状的关联分析[J]. 中国畜牧兽医, 2022, 49(11): 4279-4287.

ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.

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