梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.08.016

摘要/Abstract

摘要： 【目的】探索梅花鹿成纤维细胞因子受体2(fibroblast growth factor receptor 2,FGFR2)基因多态性及其对茸重性状的影响。【方法】应用直接测序法对梅花鹿FGFR2基因的全部外显子进行测序分析,通过MassARRAY^® SNP分型技术对314头24月龄梅花鹿进行基因分型和单倍型分析,分析FGFR2基因不同基因型和单倍型与茸重的关联性。【结果】在梅花鹿FGFR2基因中共发现12个多态性位点,其中5个位于外显子区域,且突变均未引起氨基酸改变,属于同义突变,其余7个位点均存在于内含子区域。分型结果显示,g.80975864 T>G位点未分型成功,后续对其余11个位点进行了分析,g.80943673 T>C、g.80943683 C>A及g.80938352 C>T 3个位点属于中度多态位点(0.25＜P＜0.5),其余位点均属于低度多态位点(P＜0.25)。χ²检验结果表明,g.80998742 G>A和g.80987708 G>A 2个位点偏离Hardy-Weinberg平衡(P＜0.05),其他9个位点均处于Hardy-Weinberg平衡(P＞0.05)。关联分析结果表明,11个多态性位点各基因型之间的茸重差异均不显著(P＞0.05)。单倍型结果显示,FGFR2基因存在5种单倍型,不同单倍型间梅花鹿茸重差异均不显著(P＞0.05)。【结论】FGFR2基因的11个突变位点可能不是影响梅花鹿茸重性状的关键位点。

关键词: 梅花鹿; 成纤维细胞因子受体2(FGFR2)基因; SNP; 鹿茸重量

Abstract: 【Objective】 This study was performed to explore the polymorphism of fibroblast growth factor receptor 2 (FGFR2) gene in sika deer and its effect on antler weight traits.【Method】 All exons of FGFR2 gene of sika deer were sequenced and analyzed by direct sequencing.314 24-month-old sika deer were genotyped and haplotyped by MassARRAY^® SNP typing technology,and the correlation between different genotypes and haplotypes of FGFR2 gene and antler weight traits was analyzed.【Result】 A total of 12 SNPs were found in the FGFR2 gene in sika deer,of which 5 were located in the exon region,and none of the mutations caused amino acid changes,belonging to synonymous mutations,and the other 7 SNPs were located in the intron region.The typing results showed that g.80975864 T>G site was not successfully typing,and the remaining 11 SNPs were subjected to subsequent analysis.The 3 SNPs of g.80943673 T>C,g.80943683 C>A and g.80938352 C>T belonged to moderately polymorphic (0.25<PIC<0.5),and the rest belonged to low-polymorphism (PIC<0.25). χ² fitness test results showed that the 2 SNPs of g.80998742 G>A and g.80987708 G>A deviated from Hardy-Weinberg equilibrium (P<0.05),and the others were in Hardy-Weinberg equilibrium.(P>0.05).The results of association analysis showed that there was no significant difference in antler weight among the genotypes of the 11 SNPs (P>0.05).Haplotype results showed that there were five haplotypes in FGFR2 gene,and there was no significant difference between different haplotypes and antler weights (P>0.05).【Conclusion】 The 11 loci at FGFR2 were not the potential markers for antler weight.

Key words: sika deer; fibroblast growth factor receptor 2 (FGFR2) gene; SNP; antler weight

中图分类号:

S825

周雅, 张禾垟, 刘琳玲, 李浩东, 郑军军, 王桂武. 梅花鹿成纤维细胞因子受体2基因多态性及其与茸重性状的关联分析[J]. 中国畜牧兽医, 2022, 49(8): 3006-3014.

ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Analysis of FGFR2 Gene Polymorphism and Their Correlation with Antler Weight Traits in Sika Deer[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3006-3014.

参考文献

[1] SUI Z,ZHANG L,HUO Y,et al.Bioactive components of velvet antlers and their pharmacological properties[J].Journal of Pharmaceutical and Biomedical Analysis,2014,87:229-240.
[2] 李勋胜,赵海平,李春义,等.鹿茸神经再生的研究进展[J].生命科学,2021,33(8):955-961. LI X S,ZHAO H P,LI C Y,et al.Research progress of deer antler nerve regeneration[J].Chinese Bulletin of Life Sciences,2021,33(8):955-961.(in Chinese)
[3] JOHNSON D E,LEE P L,LU J,et al.Diverse forms of a receptor foracidic and basic fibroblast growth factors[J].Molecular and Cellular Biology,1990,10:4728-4736.
[4] OLSEN S K,IBRAHIMI O A,RAUCCI A,et al.Insights into the molecular basis for fibroblast growth factor receptor autoinhibition and ligand-binding promiscuity[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(4):935-940.
[5] ESWARAKUMAR V P,LAX I,SCHLESSINGER J.Cellular signaling by fibroblast growth factor receptors[J].Cytokine and Growth Factor Reviews,2005,16(2):139-149.
[6] 徐伟.FGFR2在颅骨缺损修复和骨形成中的作用研究[D].重庆:第三军医大学,2014. XU W.Role of FGFR2 in calvarial defect healing and bone formation[D].Chongqing:Third Military Medical University,2014.(in Chinese)
[7] WANG Y,SUN M,UHLHORN V L,et al.Activation of p38 MAPK pathway in the skull abnormalities of Apert syndrome Fgfr2^+P253R mice[J].BioMed Central Developmental Biology,2010,10:22.
[8] BEENKEN A,MOHAMMADI M.The FGF family:Biology,pathophysiology and therapy[J].Nature Reviews Drug Discovery,2009,8(3):235-253.
[9] LAI A K,HOU W L,VERDON D J,et al.The distribution of the growth factors FGF-2 and VEGF,and their receptors,in growing red deer antler[J].Tissue and Cell,2007,39(1):35-46.
[10] 吴晋强,曹校瑞,闫瑞琴,等.FGF21及其受体FGFR1和FGFR2在小鼠毛囊第1生长周期的表达[J].畜牧兽医学报,2019,50(3):534-543. WU J Q,CAO X R,YAN R Q,et al.Expression of FGF21 and receptors FGFR1,FGFR2 in the first hair follicle growth cycle of mice[J].Acta Veterinaria et Zootechnica Sinica,2019,50(3):534-543.(in Chinese)
[11] RICHARDSON G D,BAZZI H,FANTAUZZO K A,et al.KGF and EGF signalling block hair follicle induction and promote interfollicular epidermal fate in developing mouse skin[J].Development,2009,136(13):2153-2164.
[12] HU P,DENG Y,BA H,et al.Association analysis of thirty-one single nucleotide polymorphisms with antler weight in sika deer[J].Animal Genetics,2020,51(6):990-991.
[13] ZHANG R R,LI Y,XING X M.Comparative antler proteome of sika deer from different developmental stages[J].Scientific Reports,2021,11(1):10484.
[14] 胡鹏飞,徐佳萍,艾成,等.利用全基因组重测序分析鹿茸重量相关基因[J].遗传,2017,39(11):1090-1101. HU P F,XU J P,AI C,et al.Screening weight related genes of velvet antlers by whole genome re-sequencing[J]. Hereditas (Beijing),2017,39(11):1090-1101.(in Chinese)
[15] JIA B,WANG G W,ZHENG J J,et al.Development of novel EST microsatellite markers for genetic diversity analysis and correlation analysis of velvet antler growth characteristics in sika deer[J]. Hereditas,2020,157(1):24.
[16] 杜智恒,白秀娟.梅花鹿生长激素基因单核苷酸多态与产茸量性状的相关性[J].遗传,2007,29(3):337-342. DU Z H,BAI X J.Association analysis between SNPs of the growth hormone gene and antler production in spotted deer[J]. Hereditas (Beijing),2007,29(3):337-342.(in Chinese)
[17] YANG F F,HUO L J,YANG L G,et al.Association between melatonin receptor 1A (MTNR1A) gene single-nucleotide polymorphisms and the velvet antler yield of sika deer[J].Molecular Biology Reports,2014,41(4):1901-1906.
[18] 徐伟.FGFR2在骨骼发育与骨形成中的作用与机制研究[D].重庆:第三军医大学,2017. XU W.The roles and mechanisms of FGFR2 in skeletal development and bone formation[D].Chongqing:Third Military Medical University,2017.(in Chinese)
[19] KATOH M,NAKAGAMA H.FGF receptors:Cancer biology and therapeutics[J].Medicinal Research Reviews,2014,34(2):280-300.
[20] FORMISANO L,LU Y,SERVETTO A,et al.Aberrant FGFR signaling mediates resistance to CDK4/6 inhibitors in ER+breast cancer[J].Nature Communications,2019,10(1):1373.
[21] SU X,ZHAN P,GAVINE P R,et al.FGFR2 amplification has prognostic significance in gastric cancer:Results from a large international multicentre study[J].British Journal of Cancer,2014,110(4):967-975.
[22] MAEHARA O,SUDA G,NATSUIZAKA M,et al.FGFR2 maintains cancer cell differentiation via AKT signaling in esophageal squamous cell carcinoma[J].Cancer Biology and Therapy,2021,22(5-6):372-380.
[23] CLEARY J M,RAGHAVAN S,WU Q,et al.FGFR2 extracellular domain in-frame deletions are therapeutically targetable genomic alterations that function as oncogenic drivers in cholangiocarcinoma[J].Cancer Discovery,2021,11(10):2488-2505.