鹅产蛋性状全基因组选择信号分析

doi:10.16431/j.cnki.1671-7236.2022.08.017

摘要/Abstract

摘要： 【目的】利用选择信号分析方法在伊犁鹅和霍尔多巴吉鹅中筛选与产蛋性状相关的候选基因。【方法】选取健康状况良好、饲养管理水平一致的2岁伊犁鹅母鹅和霍尔多巴吉鹅母鹅各24只,采集血液样本,提取基因组DNA,利用全基因组重测序技术进行选择信号检测分析,筛选出受到选择的候选区域,针对注释基因进行GO功能和KEGG通路富集分析,进一步筛选出与鹅产蛋性状相关的候选基因。【结果】全基因组重测序的平均测序深度为15.28×,与参考基因组比对率在97.31%以上。通过群体分化指数(Fst)对伊犁鹅和霍尔多巴吉鹅2个群体进行分析,共筛选到1 231个候选区域。与核苷酸多样性(Pi)进行联合分析后,伊犁鹅群体共筛选出10个候选区域,得到5个注释基因;霍尔多巴吉鹅群体中共筛选出353个候选区域,得到263个注释基因。GO功能和KEGG通路富集分析发现,初步筛选出6个可能与鹅产蛋性状相关的候选基因(BMP2、BMP6、MIS、ENO1、LIF、EP300),还发现了IL-18基因可能与禽类的免疫有关。【结论】本研究筛选出6个可能与鹅产蛋性状相关的候选基因,为揭示鹅产蛋性状的分子调控机制提供了参考。

关键词: 伊犁鹅; 霍尔多巴吉鹅; 选择信号; 产蛋性状; 候选基因

Abstract: 【Objective】 The aim of this study was to screen candidate genes under selection associated with egg laying traits in Yili and Hortobágy geese using selection signal analysis.【Method】 Twenty-four adult Yili geese and Twenty-four adult Hortobágy geese with good health status and consistent feeding and management level were selected in this experiment.Blood samples were collected from the geese,genomic DNA was extracted,and whole genome resequencing technology was used for selection signal detection and analysis to select the candidate regions under selection,localized to the annotated genes,and then GO function and KEGG pathway enrichment analysis were performed to further screen the candidate genes related to laying traits in goose.【Result】 The average sequencing depth of whole genome resequencing was 15.28×,and the contrast rate with the reference genome was more than 97.31%.These two populations were analyzed by population differentiation index (Fst),and a total of 1 231 candidate regions were identified.After combined analysis with nucleotide diversity (Pi),a total of 10 candidate regions were selected from Yili geese population,and 5 annotated genes were obtained;A total of 353 candidate regions were selected from Hortobágy geese population,and 263 annotated genes were obtained.GO function and KEGG pathway enrichment analysis results showed that 6 candidate genes (BMP2,BMP6,MIS,ENO1,LIF,EP300) might be related to egg-laying traits in geese were initially selected,and IL-18 gene was also found to be possibly related to the avian immunity.【Conclusion】 The results of this study screened 6 candidate genes that might be associated with egg-laying traits in geese,and provided a bioinformatic reference for revealing the molecular regulatory mechanisms of egg-laying traits in geese.

Key words: Yili geese; Hortobágy geese; selection signal; egg-laying traits; candidate genes

中图分类号:

唐碧徽, 潘璐, 李海英, 赵晓钰, 吴盈萍, 蒋腾, 丁雅文, 吴利楠, 曹妍, 梅志勇. 鹅产蛋性状全基因组选择信号分析[J]. 中国畜牧兽医, 2022, 49(8): 3015-3025.

TANG Bihui, PAN Lu, LI Haiying, ZHAO Xiaoyu, WU Yingping, JIANG Teng, DING Yawen, WU Linan, CAO Yan, MEI Zhiyong. Genome-wide Selection Signal Analysis of Laying Traits in Geese[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3015-3025.

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