巴马香猪产活仔数性状全基因组关联分析

doi:10.16431/j.cnki.1671-7236.2020.12.019

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (12): 3965-3975.doi: 10.16431/j.cnki.1671-7236.2020.12.019

巴马香猪产活仔数性状全基因组关联分析

莫家远¹, 高九昱¹, 奉玲丽¹, 李月月¹, 田威龙¹, 刘笑笑¹, 程锋¹, 梁靓¹, 雷树桥², 文蔚³, 梁晶¹, 兰干球¹

1. 广西大学动物科学技术学院, 南宁 530004;
2. 广西里建桂宁种猪有限公司, 南宁 530105;
3. 巴马原种香猪农牧实业有限公司, 河池 547500

收稿日期:2020-06-11 出版日期:2020-12-20 发布日期:2020-12-18
通讯作者: 梁晶, 兰干球 E-mail:liangjing@gxu.edu.cn;gqlan@gxu.edu.cn
作者简介:莫家远(1995-),男,广东阳春人,硕士生,研究方向:猪遗传育种,E-mail:1757355155@qq.com
基金资助:
国家现代农业产业技术体系（nycytxgxcxtd-15-01）；广西创新驱动发展专项（桂科AA17204029）；广西科技重大专项（桂科AA17292002）

Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs

MO Jiayuan¹, GAO Jiuyu¹, FENG Lingli¹, LI Yueyue¹, TIAN Weilong¹, LIU Xiaoxiao¹, CHENG Feng¹, LIANG Liang¹, LEI Shuqiao², WEN Wei³, LIANG Jing¹, LAN Ganqiu¹

1. College of Animal Science and Technology, Guangxi University, Nanning 530004, China;
2. Guangxi Lijian Guining Breeding Swine Co., Ltd., Nanning 530105, China;
3. Bama Xiang Pig Agriculture and Animal Husbandry Co., Ltd., Hechi 547500, China

Received:2020-06-11 Online:2020-12-20 Published:2020-12-18

摘要/Abstract

摘要： 为寻找与巴马香猪产活仔数相关的分子标记，试验利用全基因组关联分析（GWAS）定位并筛选了影响产活仔数性状的候选基因，采集297头具有多胎产仔记录的巴马香猪耳组织样品，提取DNA并利用猪50K SNP芯片进行基因分型，分型结果经质控与基因型填充后，使用Tassel软件对巴马香猪产活仔数性状进行全基因组关联分析。结果显示，巴马香猪平均窝产活仔数在1~9胎内随着胎次增加逐渐升高；经质控过滤后共获得32 816个SNPs位点，利用全基因组关联分析共筛选到8个与巴马香猪产活仔数相关的SNPs位点，分别在基因组或染色体水平达到显著；对关联显著SNP位点上下游500 kb内的编码基因进行富集分析，并依据猪繁殖性状相关QTL区域及基因功能，最终筛选到4个基因（CAPZB、MSH3、CITED2和HSD17B7）作为影响巴马香猪产活仔数的候选基因。

关键词: 巴马香猪; 产活仔数; 全基因组关联分析(GWAS); 候选基因; 芯片

Abstract: In order to identify the molecular markers related to alive litter size of Bama Xiang pigs,the genome-wide association study (GWAS) was used to map and screen the candidate genes affecting the alive litter size trait.Ear tissue samples of 297 Bama Xiang pigs with multiple parity records were collected,and DNA was extracted and genotyped by porcine 50K SNP beadchip.After quality control and genotype imputation,the alive litter size of Bama Xiang pigs were GWAS by Tassel.The results showed that the average number born alive per litter of Bama Xiang pigs increased gradually with the increasing of parity in the range of 1-9 parities.A total of 32 816 SNPs were obtained after quality control and filtration.8 SNPs related to alive litter size of Bama Xiang pigs were screened by genome-wide association analysis,which were significant at genome or chromosome level.Based on the enrichment analysis of the coding genes in the region between 500 kb upstream and downstream of the associated significant SNP loci,and the QTL regions and gene functions related to porcine reproductive traits,4 genes (CAPZB,MSH3,CITED2 and HSD17B7) were finally identified to be candidate genes related to alive litter size of Bama Xiang pigs.

Key words: Bama Xiang pigs; alive litter size; genome-wide association study (GWAS); candidate gene; chip

中图分类号:

S813.3

莫家远, 高九昱, 奉玲丽, 李月月, 田威龙, 刘笑笑, 程锋, 梁靓, 雷树桥, 文蔚, 梁晶, 兰干球. 巴马香猪产活仔数性状全基因组关联分析[J]. 中国畜牧兽医, 2020, 47(12): 3965-3975.

MO Jiayuan, GAO Jiuyu, FENG Lingli, LI Yueyue, TIAN Weilong, LIU Xiaoxiao, CHENG Feng, LIANG Liang, LEI Shuqiao, WEN Wei, LIANG Jing, LAN Ganqiu. Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(12): 3965-3975.

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巴马香猪产活仔数性状全基因组关联分析

Genome-wide Association Study on Alive Litter Size Trait in Bama Xiang Pigs

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