鸽GnIH基因多态性及其与产蛋性状的关联分析

doi:10.16431/j.cnki.1671-7236.2023.04.013

摘要/Abstract

摘要： 【目的】探究鸽促性腺激素抑制激素(gonadotropin-inhibitory hormone,GnIH)基因多态性,筛选影响母鸽产蛋性状的显著单核苷酸多态性(single nucleotide polymorphism,SNP)位点,用于高产母鸽的早期选择。【方法】本试验以78对白羽王鸽为研究对象,根据前期克隆获得的鸽GnIH基因mRNA序列(GenBank登录号:MG589639.1)设计引物,通过PCR扩增和直接测序法检测GnIH基因SNP位点并分型,使用Haploview软件对GnIH基因SNP进行连锁不平衡分析,使用SPSS 22.0软件对GnIH基因多态性与白羽王鸽产蛋性状进行关联分析。【结果】白羽王鸽GnIH基因中共检出7个SNPs,分别位于外显子1(c.59 C＞T、c.72 T＞A)、外显子2(c.398 G＞C)和3'-UTR (c.768 C＞T、c.860 G＞A、c.909 A＞G、c.961 T＞C),除c.59 C＞T位点偏离Hardy-Weinberg平衡外(P＜0.05),其余位点均处于Hardy-Weinberg平衡状态(P＞0.05);c.768 C＞T与c.961 T＞C位点呈完全连锁不平衡状态(D’=1,r²=1),c.860 G＞A与c.768 C＞T、c.961 T＞C位点呈强连锁状态(D’=1,r²=0.69),其余位点呈弱连锁状态;c.398 G＞C位点突变造成了氨基酸性质和mRNA二级结构的改变,该位点与母鸽年产蛋量和初产体重均呈显著相关(P＜0.05)。【结论】GnIH基因外显子2上的SNP位点对母鸽产蛋性状有显著影响,可作为母鸽产蛋性能的候选分子标记。

关键词: 鸽; GnIH基因; 单核苷酸多态性(SNP); 产蛋性状; 关联分析

Abstract: 【Objective】 This study was aimed to investigate the polymorphism of the gonadotropin-inhibitory hormone (GnIH) gene in pigeons,and screen the single nucleotide polymorphism (SNP) that significantly affect egg-laying traits in female pigeons for early selection of high-yielding female pigeons.【Method】 A total of 78 pairs of White King pigeons was selected as the research objects,and primers were designed according to the mRNA sequence of GnIH gene in pigeons (GenBank accession No.:MG589639.1) obtained by earlier cloning,and GnIH gene was detected by PCR amplification and direct sequencing for SNP and genotyping.The linkage imbalance analysis was conducted on GnIH gene SNP using Haploview software,and the correlation between the polymorphism of GnIH gene and egg-laying trait in White King pigeons was analyzed by SPSS 22.0 software.【Result】 Seven SNPs were detected in GnIH gene of White King pigeons,which were located in exon 1 (c.59 C＞T and c.72 T＞A),exon 2 (c.398 G＞C) and 3'-UTR (c.768 C＞T,c.860 G＞A,c.909 A＞G and c.961 T＞C),respectively.Expect c.59 C＞T deviated from Hardy-Weinberg equilibrium (P＜0.05),all other SNPs were in Hardy-Weinberg equilibrium (P＞0.05).c.768 C＞T was completely linked to c.961 T＞C (D’=1 and r²=1),c.860 G＞A was strongly linked to c.768 C＞T and c.961 T＞C (D’=1 and r²=0.69),and other SNPs were weakly linked.c.398 G＞C mutation caused changes in amino acid properties and mRNA secondary structure,which was significantly correlated with annual egg production and body weight at the first egg of female pigeons (P＜0.05).【Conclusion】 The SNP on exon 2 of GnIH gene had certain genetic effects on the laying traits of pigeons,which could be used as candidate molecular marker for the egg-laying performance of female pigeons.

Key words: pigeons; GnIH gene; single nucleotide polymorphism (SNP); egg-laying traits; correlation analysis

中图分类号:

S813.3

张蕊, 穆春宇, 汤青萍, 卜柱, 杨明军, 夏爱萍, 杨晓明. 鸽GnIH基因多态性及其与产蛋性状的关联分析[J]. 中国畜牧兽医, 2023, 50(4): 1412-1421.

ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.

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