锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响

doi:10.16431/j.cnki.1671-7236.2023.06.011

摘要/Abstract

摘要： 【目的】本试验旨在研究锌及乳酸杆菌对乳鸽沙门氏菌感染的防控效果。【方法】选取90只健康、体重相近的10日龄白羽王乳鸽，随机分为5组，每组18只乳鸽。分别为对照组（C组）、感染组（S组，鼠伤寒沙门氏菌）、锌组（Zn组，硫酸锌＋鼠伤寒沙门氏菌）、乳酸杆菌组（LAB组，乳酸杆菌＋鼠伤寒沙门氏菌）、联合处理组（Zn＋LAB组，硫酸锌＋乳酸杆菌＋鼠伤寒沙门氏菌）。试验第5天进行鼠伤寒沙门氏菌攻毒模型的建立，C组灌喂1 mL去离子水，其他组各灌喂1 mL鼠伤寒沙门氏菌悬液（1×10⁹ CFU/mL），连续攻毒3 d。采集血样、肝脏，用于免疫和抗氧化指标测定；采集脾脏和法氏囊并称重，用于计算脾脏指数和法氏囊指数；采集空肠和回肠组织，用于组织切片的制作。【结果】与对照组相比，感染组乳鸽回肠绒毛高度/隐窝深度值比（V/C）显著降低（P<0.05）。与感染组相比，锌组和乳酸杆菌组乳鸽单核细胞比率显著降低（P<0.05）；联合处理组乳鸽空肠V/C、回肠V/C和血清T-SOD活性显著提高（P<0.05）。【结论】乳鸽灌服锌或联合灌服低剂量锌与乳酸杆菌均可通过提高脾脏器官指数及机体抗氧化能力、改善肠道组织形态结构抑制沙门氏菌在机体的系统性感染，且联合灌服效果更好。

关键词: 锌; 乳酸杆菌; 鼠伤寒沙门氏菌; 乳鸽; 免疫; 抗氧化

Abstract: 【Objective】 This experiment was designed to study the effect of zinc and Lactobacillus on the prevention and control of Salmonella infection in squabs.【Method】 Ninety White feather King pigeons of 10 days of age were selected and randomly divided into 5 groups, with 18 pigeons in each group.They were control group (C group), infection group (S group, Salmonella Typhimurium), zinc group (Zn group, zinc sulfate+Salmonella Typhimurium), Lactobacillus group (LAB group, Lactobacillus), and combined treatment group (Zn+LAB group, zinc sulfate+Lactobacillus+Salmonella Typhimurium).On the 5th day of the test, the model of Salmonella Typhimurium challenge was established.The pigeons in group C was fed 1 mL PBS as the control, and other groups were fed 1 mL Salmonella Typhimurium suspension (1×10⁹ CFU/mL) for 3 consecutive days.Blood samples and livers were collected for the determination of immune and antioxidant indexes.Spleen and bursae of Fabricius were collected and weighed to calculate spleen index and bursae index of Fabricius.Jejunum and ileum tissues were collected for the preparation of tissue sections.【Result】 Compared with the control group, the ratio of ileum villus height to crypt depth (V/C) in infected group was significantly decreased (P<0.05).Compared with infection group, the monocyte ratio of squab were significantly decreased in zinc group and Lactobacillus group (P<0.05), and jejunum V/C, ileum V/C and serum T-SOD activities were significantly increased in combined treatment group (P<0.05).【Conclusion】 The systemic infection of Salmonella in squabs could be inhibited by increasing spleen organ index and antioxidant capacity, and improving intestinal morphology by instillation of zinc or combined administration of low dose zinc and Lactobacillus, and the combined administration effects were better.

Key words: zinc; Lactobacillus; Salmonella Typhimurium; squabs; immune; antioxidant

中图分类号:

S816.7

汪阳阳, 秦士贞, 唐德富, 邵玉新, 史兆国, 王铮, 李星, 赵东栋. 锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响[J]. 中国畜牧兽医, 2023, 50(6): 2276-2285.

WANG Yangyang, QIN Shizhen, TANG Defu, SHAO Yuxin, SHI Zhaoguo, WANG Zheng, LI Xing, ZHAO Dongdong. Effects of Zinc, Lactobacillus on Immune and Antioxidant Function of Squabs Infected with Salmonella[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2276-2285.

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