中国畜牧兽医 ›› 2023, Vol. 50 ›› Issue (6): 2363-2369.doi: 10.16431/j.cnki.1671-7236.2023.06.020

• 遗传繁育 • 上一篇    下一篇

丙酮酸钠对山羊冷冻精子质量的影响

吕春荣1, 梁家充1, 邵庆勇1, 吴国权1, 张玉梅2, 权国波1   

  1. 1. 云南省畜牧兽医科学院, 国家区域性(云南省)畜禽遗传资源基因库, 云南省畜禽遗传资源保护和种质创新工程实验室, 昆明 650224;
    2. 元谋县元马镇农业农村服务中心, 元谋 651300
  • 收稿日期:2022-11-24 出版日期:2023-06-05 发布日期:2023-05-30
  • 通讯作者: 权国波 E-mail:waltq20020109@163.com
  • 作者简介:吕春荣,E-mail:chunronglv228@163.com。
  • 基金资助:
    云南省应用基础研究计划项目(202101AT070060);国家自然科学基金(31960663)

Effects of Sodium Pyruvate on the Quality of Frozen Goat Sperm

LYU Chunrong1, LIANG Jiachong1, SHAO Qingyong1, WU Guoquan1, ZHANG Yumei2, QUAN Guobo1   

  1. 1. Yunnan Provincial Laboratory of Livestock and Poultry Genetic Resources Protection and Germplasm Innovation Engineering, National Regional (Yunnan Province) Gene Bank of Livestock and Poultry Genetic Resources, Yunnan Academy of Animal Husbandry and Veterinary Sciences, Kunming 650224, China;
    2. Agricultural and Rural Service Center of Yuanma Town, Yuanmou County, Yuanmou 651300, China
  • Received:2022-11-24 Online:2023-06-05 Published:2023-05-30

摘要: 【目的】研究丙酮酸钠对山羊冻精质量的影响。【方法】采用假阴道法采集6只云上黑山羊精液,添加含不同浓度(0、1、5、10、20 μmol/L)丙酮酸钠的Optidyl稀释液稀释后进行细管分装。使用SCA全自动精液分析仪测定精子总活力(TM)、直线运动百分率(PM),通过低渗耐受性试验(HOST)检测质膜完整性,用流式细胞仪检测顶体完整性、磷脂酰丝氨酸(PS)分布和活性氧(ROS)等指标。【结果】与0 μmol/L丙酮酸钠组相比,1和5 μmol/L丙酮酸钠组精子的TM和PM均极显著改善(P<0.01),其他各处理组间无显著差异(P>0.05);1和5 μmol/L丙酮酸钠组精子曲线速度和平均路径速度、侧幅摆动、鞭打次数、质膜完整性和顶体完整率、ROS水平均极显著或显著升高(P<0.01;P<0.05)。流式分析结果表明,冷冻稀释液中丙酮酸钠浓度为1、5和10 μmol/L时,正常精子比例显著高于0 μmol/L丙酮酸钠组(P<0.05)。【结论】在稀释液中添加丙酮酸钠可有效降低冷冻解冻过程对山羊精子的损伤,冻精运动能力、结构完整性和抗氧化性均显著提升。少量添加丙酮酸钠对精子有改善作用,而过量添加则会出现负面作用。本试验条件下最佳作用浓度为1 μmol/L。

关键词: 山羊精液; 丙酮酸钠; 冷冻; 抗氧化剂; 活性氧

Abstract: 【Objective】 The aim of this study was to explore the effects of sodium pyruvate on the quality of frozen sperm of goat.【Method】 Semen of 6 Yunshang Black goats was collected by pseudoginosis method, and diluted with Optidyl diluent containing 0, 1, 5, 10 and 20 μmol/L sodium pyruvate.The total motility (TM) and progressive motility (PM) were measured by SCA automatic semen analyzer, the plasma membrane integrity was measured by hypotonic tolerance test (HOST).Acrosomal integrity, phosphatidylserine (PS) distribution and reactive oxygen species (ROS) were measured by flow cytometry.【Result】 The results showed that compared with 0 μmol/L sodium pyruvate group, the TM and PM indices of sperm in 1 and 5 μmol/L sodium pyruvate groups were extremely significantly improved (P<0.01), but there was no significant difference among other treatment groups (P>0.05). Curvilineal velocity and average velocity, slide swing, whip times, plasma membrane integrity, acrosome integrity rate, ROS level of sperm in 1 and 5 μmol/L sodium pyruvate groups were extremely significantly or significantly increased (P<0.01 or P<0.05). Flow analysis results showed that the proportion of normal sperm was significantly higher than that in 0 μmol/L sodium pyruvate group when the concentration of sodium pyruvate was 1, 5 and 10 μmol/L (P<0.05).【Conclusion】 In this study, adding sodium pyruvate into diluent could effectively reduce the damage to goat sperms during freezing and thawing, and significantly improve the locomotor ability, structural integrity and antioxidant activity of frozen sperms.A small amount of sodium pyruvate showed an improvement effect on sperm, while an excessive amount of sodium pyruvate showed a negative effect.The optimum concentration was 1 μmol/L in this experiment.

Key words: goat semen; sodium pyruvate; freeze; antioxidant; reactive oxygen species

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