延边黄牛脂蛋白脂肪酶基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.029

摘要/Abstract

摘要： 为探究延边黄牛脂蛋白脂肪酶（lipoprteinlipase，LPL）基因遗传多态性及其对肉质性状的影响，本试验以80头健康的30月龄延边黄牛背最长肌作为试验材料，运用PCR直接测序法和高分辨率熔解曲线技术（HRM）对LPL基因的SNP位点进行检测，分析了试验群体的遗传效应，并与延边黄牛的肉质性状数据进行关联分析。PCR直接测序结果表明，延边黄牛LPL基因存在2个多态性位点（g.6215 A>G和g.18341 C>T），其中g.6215 A>G位点表现为2种基因型：AA与AG，优势等位基因为A；g.18341 C>T位点表现为2种基因型：CC和CT，C为优势等位基因；对文献中2个位点的检测结果表明，g.355427 T>A位点表现为2种基因型：AA与AT，A为优势等位基因；c.322 G>A位点的HRM分型结果显示并未分型。这些多态性位点全部呈中度多态（0.25<PIC<0.5），并处于Hardy-Weinberg平衡状态。HRM分型结果显示，这3个SNPs位点与延边黄牛肉质性状存在显著相关：g.6215 A>G位点与延边黄牛胴体重及脂肪、蛋白、水分含量有显著关联，表现为AG基因型个体胴体重、脂肪含量显著高于AA基因型个体（P<0.05），AA基因型个体蛋白及水分含量显著高于AG基因型个体（P<0.05）；g.18341 C>T位点与延边黄牛宰前活重、胴体重及背膘厚有显著关联，表现为CT基因型个体这些性状均显著高于CC基因型个体（P<0.05）；g.355427 T>A位点AT基因型个体宰前活重、背膘厚和脂肪含量均高于AA基因型个体（P<0.05）。综上，LPL基因g.6215 A>G、g.18341 C>T和g.355427 T>A位点对延边黄牛的肉质有显著影响，LPL基因可以作为延边黄牛品种选育改良工作的优势候选基因和有效分子标记。

关键词: 延边黄牛; LPL基因; 肉质性状; SNP; 基因分型

Abstract: This experiment was conducted to explore the association between the polymorphism of lipoprteinlipase (LPL) gene and meat quality traits in Yanbian Yellow cattle.In this study,longissimus dorsi muscle of 80 healthy Yanbian Yellow cattle aged 30 months were used as experimental materials.The SNP of LPL gene was detected by PCR sequencing and HRM,respectively.Genetic effects of the experimental population was analyzed and correlated with the meat quality data of Yanbian Yellow cattle.The PCR sequencing results showed that there were two polymorphic loci (g.6215 A>G and g.18341 C>T) of LPL gene in Yanbian Yellow cattle.g.6215 A>G of LPL gene showed two genotypes of AA and AG,and the dominant allele was A;g.18341 C>T showed two genotypes of CC and CT,C was the dominant allele.The detection results of two loci in the reference showed that g.355427 T>A showed two genotypes of AA and AT,A was the dominant allele,and c.322 G>A,showed no genotyping.All of SNPs showed moderate polymorphism (0.25<PIC<0.5) and were in Hardy-Weinberg equilibrium.The results of HRM genotyping showed that there were three SNPs in genotyping,which were correlated with meat quality traits of Yanbian Yellow cattle.g.6215 A>G was significantly correlated with carcass weight,fat content,protein content and water content of Yanbian Yellow cattle,which were:carcass weight,fat content of AG genotype were significantly higher than AA genotype (P<0.05),and protein content,water content of AA genotype were significantly higher than AG genotype (P<0.05).g.18341 C>T was significantly correlated with pre-slaughter weight,carcass weight and backfat thickness of Yanbian Yellow cattle,which showed that CT genotype was significantly higher than CC genotype (P<0.05).In g.355427 T>A,pre-slaughter weight,backfat thickness and fat content of AT genotype were higher than AA genotype (P<0.05).To sum up,g.6215 A>G,g.18341 C>T and g.355427 T>A had an effect on the meat quality of Yanbian Yellow cattle,and LPL gene could be used as the dominant candidate gene and effective molecular marker for breeding and improvement of Yanbian Yellow cattle breeds.

Key words: Yanbian Yellow cattle; LPL gene; meat quality traits; SNP; genotyping

中图分类号:

S813.3

张珈溯, 郭思玮, 张馨心, 张聪聪, 张帅, 尹宝珍, 张洛萌, 夏广军. 延边黄牛脂蛋白脂肪酶基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2021, 48(7): 2530-2538.

ZHANG Jiasu, GUO Siwei, ZHANG Xinxin, ZHANG Congcong, ZHANG Shuai, YIN Baozhen, ZHANG Luomeng, XIA Guangjun. Genetic Polymorphism of LPL gene and Its Correlation Analysis of Meat Quality Traits in Yanbian Yellow Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2530-2538.

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