MAPK信号通路2个结构变异多态性及其与香猪生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.07.020

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (7): 2448-2456.doi: 10.16431/j.cnki.1671-7236.2021.07.020

MAPK信号通路2个结构变异多态性及其与香猪生长性状的关联分析

孙镘熹¹, 陈芳¹, 王志勇¹, 刘畅³, 黄世会², 李升¹, 牛熙¹, 王嘉福¹, 冉雪琴²

1. 贵州大学生命科学学院, 农业生物工程研究院, 贵阳 550025;
2. 贵州大学动物科学学院, 贵阳 550025;
3. 贵州中医药大学药学院, 贵阳 550025

收稿日期:2021-03-05 出版日期:2021-07-20 发布日期:2021-07-15
通讯作者: 王嘉福, 冉雪琴 E-mail:jfwang@gzu.edu.cn;xqran@gzu.edu.cn
作者简介:孙镘熹(1995-),女,贵州凯里人,硕士生,研究方向:生物工程,E-mail:714016353@qq.com
基金资助:
国家高技术研究发展计划（863计划）课题（2013AA102503）；国家自然科学基金（31960641、31672390）；贵州省"百"层次创新型人才培养项目（黔科合人才[2016]4012号）；贵州省主要畜禽基因组大数据分析与应用研究科技创新人才团队（黔科合平台人才[2019]5615）；贵州省科技支撑计划（黔科合支撑[2017]2585、黔科合支撑[2017]2587）；贵州省科技计划项目（黔科合LH字[2017]7273号）

Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs

SUN Manxi¹, CHEN Fang¹, WANG Zhiyong¹, LIU Chang³, HUANG Shihui², LI Sheng¹, NIU Xi¹, WANG Jiafu¹, RAN Xueqin²

1. Institute of Agricultural Bioengineering, College of Life Sciences, Guizhou University, Guiyang 550025, China;
2. College of Animal Science, Guizhou University, Guiyang 550025, China;
3. Guizhou University of Traditional Chinese Medicine School of Pharmacy, Guiyang 550025, China

Received:2021-03-05 Online:2021-07-20 Published:2021-07-15

摘要/Abstract

摘要： 试验旨在探究丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）信号通路中MAPK10、MEF2C基因结构变异的遗传多样性及其与香猪生长性状的关联性，以期为贵州香猪的育种改良提供理论依据。采用PCR技术对233头香猪2个结构变异（structure variation，SV）位点（MAPK10-I₈-sv273和MEF2C-I₁-sv71）进行群体基因变异研究，利用Origin软件绘制香猪的生长曲线，利用在线软件计算出多态信息含量（PIC）、杂合度（He）、纯合度（Ho）及有效等位基因数（Ne），并利用SPSS 26.0软件分析基因分型结果与香猪生长性状的关联性。结果显示，2个结构变异位点在香猪群体中存在多态性，均有3种基因型：DD、ID和II，2种等位基因：D和I。其中，MAPK10-I₈-sv273的D等位基因频率明显高于I等位基因，DD基因型频率最高（0.65），且DD基因型个体的平均日增重、体高和生长速度均大于II基因型个体，表明该位点D等位基因具有提高香猪生长性能的遗传效应；MEF2C-I₁-sv71的I等位基因频率高于D等位基因，II基因型频率最高（0.51），II基因型个体的平均日增重、胸围、总体生长速度均大于DD基因型个体，表明该位点的I等位基因可提高香猪的生长性能。综上，结构变异MAPK10-I₈-sv273和MEF2C-I₁-sv71与香猪的生长发育相关，可作为生长性状选育的候选分子标记。

关键词: 香猪; MAPK信号通路; 结构变异; 生长性状; 关联分析

Abstract: The aim of this study was to investigate the genetic diversity of MAPK10 and MEF2C gene structural variants in mitogen-activated protein kinase (MAPK) signaling pathway and its association with growth traits in Xiang pigs,in order to provide a theoretical basis for breeding improvement of Xiang pigs in Guizhou.The population genetic variation of two structure variation (SV) loci (MAPK10-I₈-sv273 and MEF2C-I₁-sv71) were investigated in 233 Xiang pigs by PCR technology,the growth curve of Xiang pigs was made by Origin software,the polymorphic information content (PIC),heterozygosity (He),homozygosity (Ho) and effective allele number (Ne) were calculated by online software,and the correlation between genotyping results of two SVs and growth traits of Xiang pigs was analyzed by SPSS 26.0 software.The results showed that two SVs were polymorphic in Xiang pig population,there were three genotypes of DD,ID and II,and two alleles of D and I.The allele frequency of D in MAPK10-I₈-sv273 was higher than I,and the genotype frequency of DD was the highest (0.65).The average daily gain,body height and growth rate of the individual with DD genotype were greater than that with II genotype,which indicated that D allele had the genetic effect to improve the growth performance in Xiang pigs.The allele frequency of I in MEF2C-I₁-sv71 was higher than D,and the genotype frequency of II was the highest (0.51).The average daily gain,chest circumference and growth rate of the individuals with II genotype were higher than that with DD genotype,which showed that I allele could improve the growth performance in Xiang pigs.In summary,MAPK10-I₈-sv273 and MEF2C-I₁-sv71 were related to the growth and development of Xiang pigs,and could be used as candidate molecular markers for the selection of growth traits.

Key words: Xiang pigs; MAPK signaling pathway; structural variation; growth traits; association analysis

中图分类号:

S813.3

孙镘熹, 陈芳, 王志勇, 刘畅, 黄世会, 李升, 牛熙, 王嘉福, 冉雪琴. MAPK信号通路2个结构变异多态性及其与香猪生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(7): 2448-2456.

SUN Manxi, CHEN Fang, WANG Zhiyong, LIU Chang, HUANG Shihui, LI Sheng, NIU Xi, WANG Jiafu, RAN Xueqin. Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2448-2456.

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MAPK信号通路2个结构变异多态性及其与香猪生长性状的关联分析

Polymorphisms of Two Structural Variant in MAPK Signaling Pathway and Its Association Analysis with Growth Traits in Xiang Pigs

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