乌珠穆沁羊DGAT1、NR6A1、MAN1A1基因多态性与其生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2021.09.022

摘要/Abstract

摘要： 试验旨在探究甘油二酯酰基转移酶（diacylglycerol acyltransferase 1，DGAT1）基因g.13504098 C>T位点、核受体辅抑制子1（nuclear receptor subfamily 6 group A member 1，NR6A1）基因g.11204707 A>C位点和α-甘露糖苷酶（alpha-mannosidase，MAN1A1）基因g.18795097 C>T位点的多态性与乌珠穆沁羊生长性状之间的关系，为高产乌珠穆沁羊分子选育提供新的遗传标记。运用Sequenom MassARRAY^®SNP技术对乌珠穆沁羊DGAT1、NR6A1和MAN1A1基因的3个多态位点g.13504098 C>T、g.11204707 A>C和g.18795097 C>T进行检测，同时利用线性混合模型分析基因型与其4、6月龄生长性状的关联性。结果显示，在这3个位点中均检测到3种基因型，具体为：g.13504098 C>T位点的基因型为：CC、CT和TT，优势基因型为CC （0.49），优势等位基因为C （0.70）；g.11204707 A>C位点的基因型为：AA、CA和CC，优势基因型为AA （0.54），优势等位基因为A （0.73）；g.18795097 C>T位点的基因型为：CC、TC和TT，优势基因型为TC （0.51），优势等位基因为C （0.55）。卡方检验显示，g.13504098 C>T、g.11204707 A>C和g.18795097 C>T位点在乌珠穆沁羊群体中均处于哈代-温伯格（Hardy-Weinberg）平衡状态（P>0.05）。群体遗传学分析表明，这3个位点在乌珠穆沁羊种群中属于中度多态性（0.25<PIC<0.50）。关联分析结果显示，g.13504098 C>T位点与乌珠穆沁羊4、6月龄的体重、体高、胸围、管围及6月龄的胸宽均呈极显著相关（P<0.01）；g.11204707 A>C位点与乌珠穆沁羊4、6月龄的体高、体斜长及4月龄的体重、6月龄的胸围均呈极显著相关（P<0.01），与6月龄的体重、管围均呈显著相关（P<0.05）；g.18795097 C>T位点与乌珠穆沁羊4月龄的体高、6月龄的胸围均呈极显著相关（P<0.01），与4月龄的体重、胸围以及6月龄的胸宽均呈显著相关（P<0.05）。综上所述，DGAT1基因g.13504098 C>T、NR6A1基因g.11204707 A>C和MAN1A1基因g.18795097 C>T位点多态性与乌珠穆沁羊群体部分生长性状具有显著的关联性，可以作为乌珠穆沁羊遗传选育的候选分子标记。

关键词: 绵羊; DGAT1基因; NR6A1基因; MAN1A1基因; 生长性状

Abstract: The aim of this study was to elucidate the polymorphisms of g. 13504098 C>T of diacylglycerol acyltransferase 1 (DGAT1) gene, g. 11204707 A>C of nuclear receptor subfamily 6 group A member 1 (NR6A1) gene and g. 18795097 C>T of alpha-mannosidase (MAN1A1) gene, and its association with the growth traits in Ujumqin sheep, providing new genetic markers for high productivity molecular breeding of Ujumqin sheep. g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T of DGAT1, NR6A1 and MAN1A1 genes in Ujumqin sheep were detected by Sequenom MassARRAY^®SNP assay, and the linear mixed model was used to analyze the association between genotypes and the growth trait in Ujumqin sheep at 4 and 6 months of age. The results showed that three genotypes were detected at three loci. Specifically, g. 13504098 C>T included CC, CT and TT genotypes, CC genotype was dominant genotype (0.49), C was the dominant allele (0.70). g. 11204707 A>C included AA, CA and CC genotypes, AA genotype was dominant genotype (0.54), A was the dominant allele (0.73). g. 18795097 C>T included CC, TC and TT genotypes, TC genotype was dominant genotype (0.51), C was the dominant allele (0.55). χ² test indicated that g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T of Ujumqin sheep were in Hardy-Weinberg equilibrium (P>0.05). Population genetic analysis showed that g. 13504098 C>T, g. 11204707 A>C and g. 18795097 C>T were at moderate polymorphisms in Ujumqin sheep population (0.25<PIC<0.50). Association analysis revealed that g. 13504098 C>T was extremely significantly associated with the body weight, body height, chest girth, shin circumference of Ujumqin sheep at 4 and 6 months of age, and the chest width at 6 months of age (P<0.01);g. 11204707 A>C was extremely significantly associated with the body height and body length of Ujumqin sheep at 4 and 6 months of age, the body weight at 4 months of age, and the chest girth at 6 months of age (P<0.01), and it was significantly associated with the body weight and shin circumference at 6 months of age (P<0.05);g. 18795097 C>T was extremely significantly associated with the body height at 4 months of age and the chest girth at 6 months of age (P<0.01), and significantly associated with the body weight and chest girth at 4 months of age, and the chest width at 6 months of age (P<0.05). Therefore, it could be concluded that g. 13504098 C>T of DGAT1 gene, g. 11204707 A>C of NR6A1 gene and g. 18795097 C>T of MAN1A1 gene had significantly correlation with the growth traits of Ujumuqin sheep, indicating that 3 SNPs could be used as candidate molecular markers for genetic breeding in Ujumuqin sheep.

Key words: sheep; DGAT1 gene; NR6A1 gene; MAN1A1 gene; growth traits

中图分类号:

S813.3

张晋华, 尚明玉, 胡文萍, 张莉. 乌珠穆沁羊DGAT1、NR6A1、MAN1A1基因多态性与其生长性状的关联分析[J]. 中国畜牧兽医, 2021, 48(9): 3312-3322.

ZHANG Jinhua, SHANG Mingyu, HU Wenping, ZHANG Li. Association Analysis Between Polymorphisms of DGAT1,NR6A1 and MAN1A1 Genes and Growth Traits in Ujumqin Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3312-3322.

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