CTGF、Gprin3基因多态性及其与牦牛体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.06.022

摘要/Abstract

摘要： 【目的】检测牦牛结缔组织生长因子（CTGF）和G蛋白调节诱导神经突增生家族亚型因子3（Gprin3）基因的单核苷酸多态性（SNP），并分析多态位点与牦牛体尺性状的关联性。【方法】以西藏的斯布牦牛、帕里牦牛、类乌齐牦牛、申扎牦牛及四川的麦洼牦牛5个类群260头个体为研究对象，利用基因池筛选技术检测CTGF、Gprin3基因SNP位点，分析其多态信息含量、有效等位基因数、纯合度及杂合度等指标，利用最小二乘线性模型分析不同基因型与牦牛体尺性状的关联性。【结果】牦牛CTGF基因存在3个SNPs位点，分别为T1537A、C2195T和C2421T，均位于内含子区，其中T1537A位点在斯布牦牛和麦洼牦牛中均处于Hardy-Weinberg平衡状态，而在帕里牦牛、类乌齐牦牛和申扎牦牛中均偏离Hardy-Weinberg平衡状态，该位点与牦牛体高呈显著相关（P<0.05）；C2195T位点在5个牦牛类群中均处于Hardy-Weinberg平衡状态，且处于中度多态（0.25<PIC<0.5）；C2421T位点在5个牦牛类群中均处于Hardy-Weinberg平衡状态，该位点与牦牛体重、体斜长、胸围、管围和前肢长均呈显著相关（P<0.05）。牦牛Gprin3基因共发现4个SNPs，分别为G310C、C414T、C1100T和G1213A，其中G310C、C414T及C1100T位点在5个牦牛类群中均处于Hardy-Weinberg平衡状态；G1213A位点在类乌齐牦牛中偏离Hardy-Weinberg平衡状态，在其余4个类群中均处于Hardy-Weinberg平衡状态；G310C位点在5个牦牛类群中均处于低度多态（PIC<0.25），C414T和C1100T位点在类乌齐牦牛、斯布牦牛中均处于中度多态，G1213A位点在类乌齐牦牛中处于中度多态，在其余牦牛类群中为低度多态；C414T位点与牦牛体重、体高、体斜长和胸围均呈显著相关，而在斯布牦牛和申扎牦牛中C1100T位点与其胸围和前肢长均呈显著相关（P<0.05）。【结论】牦牛CTGF和Gprin3基因具有丰富的多态性，CTGF基因T1537A、C2421T位点及Gprin3基因C414T、C1100T位点均与牦牛体尺性状有关，可作为影响生长发育性状的候选基因及基因座用于牦牛定向选育。

关键词: 牦牛; CTGF基因; Gprin3基因; 多态性; 体尺性状

Abstract: 【Objective】 The purpose of this experiment was to detect the single nucleotide polymorphism (SNP) of connective tissue growth factor (CTGF) and G protein-regulated inducer of neurite outgrowth family subtype 3 (Gprin3) genes in yaks, and analyze the association between SNP and body size traits in yaks.【Method】 The SNP of CTGF and Gprin3 genes were detected by gene pool screening technique in 260 individuals of Sibu, Pali, Leiwuqi, Shenzha yaks in Tibet and Maiwa yaks in Sichuan.The polymorphic information content (PIC), number of effective alleles, homozygosity and heterozygosity were analyzed, and the association between SNP and body size traits in yaks was analyzed by least square method (LMS).【Result】 Three SNPs were detected in intron region of CTGF gene in yaks, which were T1537A, C2195T and C2421T.T1537A was in Hardy-Weinberg equilibrium in Sibu and Maiwa yaks, and deviated from Hardy-Weinberg equilibrium in Pali, Leiwuqi and Shenzha yaks, which was significantly associated with body height (P<0.05).C2195T was in Hardy-Weinberg equilibrium and moderately polymorphic in 5 yak populations (0.25<PIC<0.5).C2421T was in Hardy-Weinberg equilibriumin in 5 yak populations, which was significantly associated with the body weight, body length, heart girth, circumference and forelimb length (P<0.05).Four SNPs were identified in Gprin3 gene, which were G310C, C414T, C1100T and G1213A.G310C, C414T and C1100T were in Hardy-Weinberg equilibrium in 5 yak populations, G1213A deviated from Hardy-Weinberg equilibrium in Leiwuqi yaks, and accorded with Hardy-Weinberg equilibrium in other 4 yak populations.G310C was low polymorphic in 5 yak populations (PIC<0.25).C414T and C1100T were moderately polymorphic in Leiwuqi and Sibu yaks, G1213A was moderately polymorphic in Leiwuqi yaks, but was low polymorphic in other populations.C414T was significantly associated with the body weight, body height, body length and heart girth in 5 yak populations, C1100T was significantly associated with the circumference and forelimb length in Sibu and Shenzha yaks (P<0.05).【Conclusion】 CTGF and Gprin3 genes had abundant polymorphism, T1537A and C2421T of CTGF gene, C414T and C1100T of Gprin3 gene, were associated with body size traits in yaks, which could be used as candidate genes and loci affecting the growth and development traits for directional breeding of yaks.

Key words: yaks; CTGF gene; Gprin3 gene; polymorphism; body size traits

中图分类号:

S823

柴志欣, 武志娟, 王吉坤, 张成福, 钟金城, 信金伟. CTGF、Gprin3基因多态性及其与牦牛体尺性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2216-2227.

CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.

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